Pathogen profile update: <i>Fusarium oxysporum</i>

Michielse, Caroline B.; Rep, Martijn

doi:10.1111/j.1364-3703.2009.00538.x

Cited by 646 publications

(505 citation statements)

References 134 publications

(171 reference statements)

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“…These diseases are caused by fungi in the phylum ascomycota that also includes F. oxysporum, A. nidulans, and S. cerevisiae. Some F. oxysporum strains are also known pathogens of these diseases (43). Because the glutathione system is conserved among these fungi, our finding that it detoxify elemental sulfur impacts the physiology of fungal pathogens treated with elemental sulfur fungicide, as well as future applications in controlling fungal pathogens.…”

Section: Discussionmentioning

confidence: 93%

Glutathione Reductase/Glutathione Is Responsible for Cytotoxic Elemental Sulfur Tolerance via Polysulfide Shuttle in Fungi

Sato

Shimatani

Fujita

et al. 2011

Journal of Biological Chemistry

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Fungi that can reduce elemental sulfur to sulfide are widely distributed, but the mechanism and physiological significance of the reaction have been poorly characterized. Here, we purified elemental sulfur-reductase (SR) and cloned its gene from the elemental sulfur-reducing fungus Fusarium oxysporum. We found that NADPH-glutathione reductase (GR) reduces elemental sulfur via glutathione as an intermediate. A loss-of-function mutant of the SR/GR gene generated less sulfide from elemental sulfur than the wild-type strain. Its growth was hypersensitive to elemental sulfur, and it accumulated higher levels of oxidized glutathione, indicating that the GR/glutathione system confers tolerance to cytotoxic elemental sulfur by reducing it to less harmful sulfide. The SR/GR reduced polysulfide as efficiently as elemental sulfur, which implies that soluble polysulfide shuttles reducing equivalents to exocellular insoluble elemental sulfur and generates sulfide. The ubiquitous distribution of the GR/glutathione system together with our findings that GR-deficient mutants derived from Saccharomyces cerevisiae and Aspergillus nidulans reduced less sulfur and that their growth was hypersensitive to elemental sulfur indicated a wide distribution of the system among fungi. These results indicate a novel biological function of the GR/glutathione system in elemental sulfur reduction, which is distinguishable from bacterial and archaeal mechanisms of glutathione-independent sulfur reduction.

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Section: Discussionmentioning

confidence: 93%

Glutathione Reductase/Glutathione Is Responsible for Cytotoxic Elemental Sulfur Tolerance via Polysulfide Shuttle in Fungi

Sato

Shimatani

Fujita

et al. 2011

Journal of Biological Chemistry

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“…Fusarium oxysporum is a root-infecting fungal pathogen that causes wilt disease on a broad range of economically important plant species and also the model plant Arabidopsis (Arabidopsis thaliana; Dombrecht et al, 2006;van Hemelrijck et al, 2006;Berrocal-Lobo and Molina, 2008;Michielse and Rep, 2009). This pathogen is soilborne and enters the plant initially through the roots, and subsequently colonizes the vascular tissues and xylem vessels before moving up to stem and foliar tissues (Lagopodi et al, 2002;Czymmek et al, 2007).…”

mentioning

confidence: 99%

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

et al. 2012

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The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) gene family encodes plant-specific transcriptional regulators functioning in organ development. In a screen of Arabidopsis (Arabidopsis thaliana) sequence-indexed transferred DNA insertion mutants, we found disruption of the LOB DOMAIN-CONTAINING PROTEIN20 (LBD20) gene led to increased resistance to the root-infecting vascular wilt pathogen Fusarium oxysporum. In wild-type plants, LBD20 transcripts were barely detectable in leaves but abundant in roots, where they were further induced after F. oxysporum inoculation or methyl jasmonate treatment. Induction of LBD20 expression in roots was abolished in coronatine insensitive1 (coi1) and myc2 (allelic to jasmonate insensitive1) mutants, suggesting LBD20 may function in jasmonate (JA) signaling. Consistent with this, expression of the JA-regulated THIONIN2.1 (Thi2.1) and VEGETATIVE STORAGE PROTEIN2 (VSP2) genes were up-regulated in shoots of lbd20 following treatment of roots with F. oxysporum or methyl jasmonate. However, PLANT DEFENSIN1.2 expression was unaltered, indicating a repressor role for LBD20 in a branch of the JA-signaling pathway. Plants overexpressing LBD20 (LBD20-OX) had reduced Thi2.1 and VSP2 expression. There was a significant correlation between increased LBD20 expression in the LBD20-OX lines with both Thi2.1 and VSP2 repression, and reduced survival following F. oxysporum infection. Chlorosis resulting from application of F. oxysporum culture filtrate was also reduced in lbd20 leaves relative to the wild type. Taken together, LBD20 is a F. oxysporum susceptibility gene that appears to regulate components of JA signaling downstream of COI1 and MYC2 that are required for full elicitation of F. oxysporum-and JA-dependent responses. To our knowledge, this is the first demonstration of a role for a LBD gene family member in either biotic stress or JA signaling.

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“…Studies revealed that the general pattern of colonization in soil was similar for the pathogenic strain and Fo47. However, Fo47 grew faster than the pathogenic strain and, as a consequence, colonized the rhizosphere earlier (Michielse and Rep, 2009). …”

Section: Consider Addition Of Non-antagonistic Microbes Into the Biocmentioning

confidence: 99%

Biocontrol of Fusarium wilt of banana: Key influence factors and strategies

Gang¹,

Wang²,

WeiHong³

et al. 2013

Afr. J. Microbiol. Res.

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Fusarium wilt of banana, caused by Fusarium oxyspoum f. sp. cubense (Foc), is one of the most important and destructive diseases of banana, and is known to be a major biotic limiting factor for the development of the present banana industry. Biocontrol on the destructive disease, the use of antagonist as biocontrol agents (BCAs) against Foc, constitutes an effective option for the management of the disease. The effectiveness of biocontrol agents depends on a range of biological and physico-chemical factors, including the type and properties of the biocontrol agents, the obstacles to the initial colonization of antagonists, as well as the variation factors after initial colonization. Various strategies can be implemented to optimize the biocontrol efficacy, such as the use of endophytes from banana plants as BCAs (favorably Bacillus spp.), the development of water and nutrition retaining agent, the application of proper carrier for BCAs, the restoration of soil biodiversity, and combined management of nematodes disease and Fusarium wilt. In this review, elements affecting the biocontrol efficacy of Fusarium wilt are analyzed in detail, and strategies to promote the biocontrol effects are proposed. Besides, the concept of "post-indigenousness" and "post-indigenous microbes" were firstly suggested.

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Pathogen profile update: Fusarium oxysporum

Abstract: http://www.broad.mit.edu/annotation/genome/fusarium_group/MultiHome.html; http://www.fgsc.net/Fusarium/fushome.htm; http://www.phi-base.org/query.php

Cited by 646 publications

References 134 publications

Glutathione Reductase/Glutathione Is Responsible for Cytotoxic Elemental Sulfur Tolerance via Polysulfide Shuttle in Fungi

Glutathione Reductase/Glutathione Is Responsible for Cytotoxic Elemental Sulfur Tolerance via Polysulfide Shuttle in Fungi

The Lateral Organ Boundaries Domain Transcription Factor LBD20 Functions in Fusarium Wilt Susceptibility and Jasmonate Signaling in Arabidopsis

Biocontrol of Fusarium wilt of banana: Key influence factors and strategies

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