Pathogenesis investigation of miR‐199‐5p in oral submucous fibrosis based on bioinformatics analysis

Abstract

Objectives Fibrosis diseases are one of the leading causes of suffering and death. However, no systematic investigation has been carried out on fibrosis‐related genes. Materials and Methods By querying PubMed using keywords “fibrosis” and “gene” or “protein,” we identified fibrosis‐related genes in the last decade. Bioinformatics analysis was performed by MAS 3.0 software. Key miRNA was selected to assess its relationship with oral submucous fibrosis (OSF) and fibroblast functions. Results A total of 1,310 genes related to fibrosis were identified. TGF‐β1, CTGF, MMP9, HSP47, and S1P were found to be associated with mainly fibrotic organs. In total, 244 cellular components terms, 595 molecular function terms, 1,816 cellular component terms, and 136 KEGG pathway annotations were predicted. miR‐199‐5p was selected as the key miRNA, which has higher level in OSF. Upregulated miR‐199‐5p was significantly related to OSF duration and OSF histological grade (p = 0.028 and 0.012, respectively). Overexpressive miR‐199‐5p reduced proliferation and induced apoptosis in buccal fibroblasts. Additionally, expression levels of collagen I (COL I) and III (COL III) were promoted by overexpressive miR‐199‐5p in buccal fibroblasts. Conclusion These results indicate that fibrosis‐related genes are related to a series of complex mechanisms. The characteristics of miR‐199‐5p may supply important clues for developing therapeutic strategy for OSF.

MicroRNA‑199a‑5p suppresses cell proliferation, migration and invasion by targeting ITGA3 in colorectal cancer

“…Previous studies have demonstrated that mirna dysregulation is involved in cell proliferation, invasion and tumor metastasis (36,37). Yuan et al (38) reported that elevated mir-199a-5p promoted the apoptosis and suppressed the proliferation in oral submucous fibrocytes, which was consistent with the results of the present study indicating cell proliferation inhibition. Furthermore, a previous study reported that upregulated mir-199a-5p expression alleviated sustained endoplasmic reticulum stress, and thus protected hepatocytes from bile acid-induced cell death (18).…”

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis

“…High levels of expression of mir199 are related to bleomycin-induced lung fibrosis in sheep and murine models and myofibroblast differentiation in oral submucous fibrosis with an increment of COL1A1 and COL3A1 (Yuan et al. 2019 ; Perera et al. 2021 ), and dysregulation of mir433 results in inhibition of AZIN1, a regulator of ECM production, with subsequent amplification of the TGFβ-1 signaling pathway (Li et al.…”