Pathogenesis of Mycobacterium bovis infection in cattle

Neill, S.D.; Pollock, J.M.; Bryson, D.G.; Hanna, J

doi:10.1016/0378-1135(94)90045-0

Cited by 174 publications

(124 citation statements)

References 47 publications

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“…However, the process by which exposure to infection can lead to a range of disease outcomes is not fully understood (Neill et al, 1994;Pollock et al, 2006). Not all infected cattle display gross lesions, but if present, they are often located in thoracic lymph nodes (Whipple et al, 1996) even in apparently healthy cattle.…”

Section: Introductionmentioning

confidence: 99%

Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

Proaño-Pérez

Benítez‐Ortiz

Desmecht

et al. 2011

Preventive Veterinary Medicine

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a b s t r a c tVeterinary inspection in slaughterhouses allows for the detection of macroscopic lesions reminiscent of bovine tuberculosis, but the presence of Mycobacterium bovis must be confirmed by laboratory methods. This study aimed at comparing the performances of the standard diagnostic tools used to identify M. bovis in tissue specimens sampled from suspicious animals. During a two years period, 1390 cattle were inspected at the Machachi abattoir in the Mejia canton -Ecuador. A total of 33 animals with granulomatous lesions were detected, representing 2.33% (16/687) and 2.42% (17/703) animals examined in 2007 and 2008, respectively. Ninety-four tissue specimens were sampled and screened for the presence of mycobacteria. Acid-fast bacilli were identified in one third of the suspicious cattle (11/33) and suggestive microscopic lesions in 27.3% (9/33) of the samples examined by direct microscopy and histopathology, respectively. Culturing on Stonebrink medium and 16S-rRNA-based polymerase chain reaction (PCR) yielded 36.4% (12/33) and 27.3% (9/33) of positives, respectively. Compared to culture, other diagnostic procedures displayed a lower sensitivity, with 56.5% for PCR, and 43.5% for direct microscopy and histopathology; however, the specificity was higher (94.4% for PCR and microscopy, and 97.2% for histopathology). We conclude that reliable post-mortem laboratory testing either requires the combination of a set of available diagnostic tools or necessitates the development of improved new-generation tools with better sensitivity and specificity characteristics.

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Section: Introductionmentioning

confidence: 99%

Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

Proaño-Pérez

Benítez‐Ortiz

Desmecht

et al. 2011

Preventive Veterinary Medicine

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“…In common with other mycobacterial infections, a spectrum of immune responses is known to exist in M. bovis infection in cattle, with cell-mediated immune responses (CMI) predominating in the early stages and antibody developing later in infection [6,7]. For this reason, the tuberculin test and more recently the interferon-g (IFN-g) assay, both measures of CMI, have been employed for the diagnosis of infected animals within eradication programmes [8,9].…”

Section: Introductionmentioning

confidence: 99%

T‐Cell Recognition of Mycobacterial Proteins MPB70 and MPB64 in Cattle Immunized with Antigen and Infected with Mycobacterium bovis

Mackie

et al. 1998

Scand J Immunol

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Lightbody KA, Girvin RM, Mackie DP, Neill SD, Pollock JM. T-Cell Recognition of Mycobacterial Proteins MPB70 and MPB64 in Cattle Immunized with Antigen and Infected with Mycobacterium bovis. Scand J Immunol 1998;48:44-51 Defined antigenic reagents and knowledge of T-cell responses are required for the design of improved diagnostic tests for bovine tuberculosis. The limited species distribution of Mycobacterium bovis antigens MPB70 and MPB64 has indicated their potential for inclusion in future tests. The strategy adopted in this study was to define bovine T-cell responses to these antigens at the epitope level, using cattle immunized with recombinant forms of the antigens, and to compare these responses with cattle which had been experimentally infected with M. bovis. Panels of synthetic peptides (20-mers with 10-residue overlaps) were used and five epitopes were identified and found to be powerful stimulators of T-cell responses in both types of animal (residues 81-100 and 174-190 for MPB70; and residues 1-20, 41-60 and 181-200 for MPB64). Further investigation in larger numbers of cattle (n ¼ 14) of mixed breeds from tuberculosisinfected herds confirmed that each peptide produced response in several of the cattle, but no single peptide was recognized by all animals. However, the limited numbers of animals in this study suggest that peptide reagents may identify as many positive animals as the intact antigenic protein and could form components of a future diagnostic test. The use of cattle immunized with the proteins of interest has proved to be an interesting model for studying the nature of bovine T-cell responses to defined mycobacterial proteins.

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“…In our study, the combination in parallel of CFT and RT ranked higher in sensitivity than did CFT alone. Considering the inverse relationship between cell-mediated and humoral responses to M. bovis (Plackett et al 1989;Neill et al 1994), it might be impossible to develop one serologic test that outperforms a combination of CFT and a serologic test in detecting animals at different stages of infection.…”

Section: Discussionmentioning

confidence: 99%

BAYESIAN ANALYSIS TO EVALUATE TESTS FOR THE DETECTION OF MYCOBACTERIUM BOVIS INFECTION IN FREE-RANGING WILD BISON (BISON BISON ATHABASCAE) IN THE ABSENCE OF A GOLD STANDARD

Chapinal

Schumaker

Joly³

et al. 2015

Journal of Wildlife Diseases

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ABSTRACT:We estimated the sensitivity and specificity of the caudal-fold skin test (CFT), the fluorescent polarization assay (FPA), and the rapid lateral-flow test (RT) for the detection of Mycobacterium bovis in free-ranging wild wood bison (Bison bison athabascae), in the absence of a gold standard, by using Bayesian analysis, and then used those estimates to forecast the performance of a pairwise combination of tests in parallel. In 1998-99, 212 wood bison from Wood Buffalo National Park (Canada) were tested for M. bovis infection using CFT and two serologic tests (FPA and RT). The sensitivity and specificity of each test were estimated using a three-test, one-population, Bayesian model allowing for conditional dependence between FPA and RT. The sensitivity and specificity of the combination of CFT and each serologic test in parallel were calculated assuming conditional independence. The test performance estimates were influenced by the prior values chosen. However, the rank of tests and combinations of tests based on those estimates remained constant. The CFT was the most sensitive test and the FPA was the least sensitive, whereas RT was the most specific test and CFT was the least specific. In conclusion, given the fact that gold standards for the detection of M. bovis are imperfect and difficult to obtain in the field, Bayesian analysis holds promise as a tool to rank tests and combinations of tests based on their performance. Combining a skin test with an animal-side serologic test, such as RT, increases sensitivity in the detection of M. bovis and is a good approach to enhance disease eradication or control in wild bison.

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Pathogenesis of Mycobacterium bovis infection in cattle

Cited by 174 publications

References 47 publications

Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

T‐Cell Recognition of Mycobacterial Proteins MPB70 and MPB64 in Cattle Immunized with Antigen and Infected with Mycobacterium bovis

BAYESIAN ANALYSIS TO EVALUATE TESTS FOR THE DETECTION OF MYCOBACTERIUM BOVIS INFECTION IN FREE-RANGING WILD BISON (BISON BISON ATHABASCAE) IN THE ABSENCE OF A GOLD STANDARD

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