Pathogenesis-related proteins in somatic hybrid rice induced by bacterial blight

Yu, Chu L.; Yan, Shun; Wang, Chang C.; Hu, Hai T.; Sun, Wei N.; Yan, Cheng; Chen, Jian P.; Yang, Li‐Tao

doi:10.1016/j.phytochem.2008.04.006

Cited by 24 publications

(29 citation statements)

References 35 publications

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“…Ribulose 1-5 bisphosphate-carboxylase (Rubisco, spot #12) is an important enzyme involved in carbon assimilation and is the largest functional category of proteins involved in photosynthesis [29]. Rubisco activase, a molecular chaperone, is reported to catalyze Rubisco from an inactive closed conformation to an active open conformation [30].…”

Section: Characterization Of Uniquely Expressed Proteins In Response mentioning

confidence: 99%

Characterization of Unique and Differentially Expressed Proteins in Anthracnose-Tolerant Florida Hybrid Bunch Grapes

Vasanthaiah

Katam

Basha

2008

Appl Biochem Biotechnol

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Anthracnose is a major disease in Florida hybrid bunch grapes, caused by a fungus viz. Elsinoe ampelina. Florida hybrid bunch grapes are grown in southeastern USA for their superior wine characteristics. However, the effect of anthracnose on grape productivity and wine quality is a major concern to grape growers. Our research is aimed at determining biochemical basis of anthracnose tolerance in Florida hybrid bunch grape. Leaf samples were collected from the plants infected with E. ampelina at different periods and analyzed for differential protein expression using high throughput two-dimensional gel electrophoresis. Among the 32 differentially expressed leaf proteins, two were uniquely expressed in tolerant genotypes in response to E. ampelina infection. These proteins were identified as mitochondrial adenosine triphosphate synthase and glutamine synthetase, which are known to play a major role in carbohydrate metabolism and defense. Several proteins including ribulose 1-5 bisphosphate-carboxylase involved in photosynthesis were found to be suppressed in susceptible genotypes compared to tolerant genotypes following E. ampelina infection. The results indicate that the anthracnose-tolerant genotypes have the ability to up-regulate and induce new proteins upon infection to defend the invasion of the pathogen as well as maintain the normal regulatory processes.

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Section: Characterization Of Uniquely Expressed Proteins In Response mentioning

confidence: 99%

Characterization of Unique and Differentially Expressed Proteins in Anthracnose-Tolerant Florida Hybrid Bunch Grapes

Vasanthaiah

Katam

Basha

2008

Appl Biochem Biotechnol

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“…Activation of RuBisCO and other photosynthesis-related proteins was found to be quite common in stress-induced plants which was observed both in susceptible/resistant host and compatible/incompatible interaction (Mahmood et al 2006;Yu et al 2008;Zimaro et al 2011). RuBisCO activase (spot=8) catalyzes the activation of RuBisCO that was also induced in PB1 (Portis 1992).…”

Section: Energy and Metabolism-related Proteinsmentioning

confidence: 99%

Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection

Kumar

Bimolata

Kannan

et al. 2015

Funct Integr Genomics

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Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice and brutally affects the yield up to 50 % of total production. Here, we report a comparative proteomics analysis of total foliar protein isolated from infected rice leaves of susceptible Pusa Basmati 1 (PB1) and resistant Oryza longistaminata genotypes. Two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) approaches identified 29 protein spots encoding unique proteins from both the genotypes. Identified proteins belonged to a large number of biological and molecular functions related to biotic and abiotic stress proteins which are potentially involved during Xoo infection. Biotic and abiotic stress-related proteins were induced during Xoo infection, indicating the activation of common stress pathway during bacterial blight infection. Candidate genes conferring tolerance against bacterial blight, which include germin-like protein, putative r40c1, cyclin-dependent kinase C, Ent-isokaur-15-ene synthase and glutathione-dependent dehydroascorbate reductase 1 (GSH-DHAR1), were also induced, with germin-like proteins induced only in the resistant rice genotype O. longistaminata. Energy, metabolism and hypothetical proteins were common among both the genotypes. Further, host defence/stress-related proteins were mostly expressed in resistant genotype O. longistaminata, indicating possible co-evolution of the pathogen and the wild rice, O. longistaminata.

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“…Two-dimensional electrophoresis (2-DE) was performed as described by Yu et al (2008). At least triplicate gels were performed for each sample.…”

Section: Protein Extraction and Electrophoresismentioning

confidence: 99%

“…Proteomics offers a powerful approach to discover the proteins and pathways that are crucial for stress responsiveness and tolerance (Yan et al 2006;Yu et al 2008). In recent years, several proteomic studies have been carried out on water stress on rice (Salekdeh et al 2002;Rabello et al 2008;Choudhary et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Proteomics to identify pathogenesis-related proteins in rice roots under water deficit

Yang

et al. 2011

Biologia

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Upland and lowland rice (Oryza sativa L.) showed different mechanisms of water stress resistance. Hydroponically grown 3-week-old seedlings of a lowland variety IR64 and an upland variety were exposed to 15% polyethylene glycol (PEG-6000). After 7 d of treatment, IR64 maintained high relative water content and developed a well-branched root. Therefore, IR64 had better water-deficit tolerance than Azucena under PEG treatment. To identify water-deficit-responsive proteins associated with the tolerance differences between two ecotypes, a comparative proteomic analysis of roots was conducted. Out of 700 proteins reproducibly detected on two-dimensional electrophoresis gels, 65 proteins exhibited significant changes in at least one ecotype at 48 h of water deficit. Only 15 proteins showed different responses to water deficit between the two ecotypes. Twelve proteins were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry, which involved in energy and metabolism, protein processing and degradation, detoxification and pathogenrelated (PR) proteins, i.e. PR-1a, RSOsPR10 and JIOsPR10. All three PR proteins were induced more strongly in IR64 than in Azucena by water deficit at both protein and mRNA level. The results suggested that PR-1a, RSOsPR10 and JIOsPR10 may play important roles in protecting root cells against water deficit in rice.

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Pathogenesis-related proteins in somatic hybrid rice induced by bacterial blight

Cited by 24 publications

References 35 publications

Characterization of Unique and Differentially Expressed Proteins in Anthracnose-Tolerant Florida Hybrid Bunch Grapes

Characterization of Unique and Differentially Expressed Proteins in Anthracnose-Tolerant Florida Hybrid Bunch Grapes

Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection

Proteomics to identify pathogenesis-related proteins in rice roots under water deficit

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