Pathogenic endoparasites of the spotted seatrout, Cynoscion nebulosus: patterns of infection in estuaries of South Carolina, USA

Arnott, Stephen A.; Dyková, Iva; Roumillat, William A.; Buron, Isaure de

doi:10.1007/s00436-017-5449-3

Cited by 9 publications

(2 citation statements)

References 144 publications

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“…are aquatic. Aquatic mesomycetozoans infect fish and amphibians [50–53] and cause dermal disease. Mesomycetozoa may also form symbioses with their hosts (e.g.…”

Section: Resultsmentioning

confidence: 99%

Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three New Zealand asteroid species

Hewson

Sewell

2020

Preprint

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Asteroid wasting events and mass mortality have occurred for over a century. We currently lack a fundamental understanding of the microbial ecology of asteroid disease, with disease investigations hindered by sparse information about the microorganisms associated with grossly normal specimens. We surveilled viruses and protists associated with grossly normal specimens of three asteroid species (Patiriella regularis, Stichaster australis, Coscinasterias muricata) on the North Island, New Zealand, using metagenomes prepared from virus and ribosome-sized material. We discovered several densovirus-like genome fragments in our RNA and DNA metagenomic libraries. Subsequent survey of their prevalence within populations by quantitative PCR (qPCR) demonstrated their occurrence in only a few (13 %) specimens (n = 36). Survey of large and small subunit rRNAs in metagenomes revealed the presence of a mesomycete (most closely matching Ichthyosporea sp.). Survey of large subunit prevalence and load by qPCR revealed that it is widely detectable (80%) and present predominately in body wall tissues across all 3 species of asteroid. Our results raise interesting questions about the roles of these microbiome constituents in host ecology and pathogenesis under changing ocean conditions.

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“…are aquatic. Aquatic mesomycetozoans infect fish and amphibians [50–53] and cause dermal disease. Mesomycetozoa may also form symbioses with their hosts (e.g.…”

Section: Resultsmentioning

confidence: 99%

Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three New Zealand asteroid species

Hewson

Sewell

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…are aquatic. Aquatic mesomycetozoans infect fish and amphibians [56][57][58][59] and cause dermal disease. Mesomycetozoa may also form symbioses with their hosts (e.g.…”

Section: Plos Onementioning

confidence: 99%

Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three Aotearoa New Zealand asteroid species

Hewson

Sewell

2021

PLoS ONE

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Asteroid wasting events and mass mortality have occurred for over a century. We currently lack a fundamental understanding of the microbial ecology of asteroid disease, with disease investigations hindered by sparse information about the microorganisms associated with grossly normal specimens. We surveilled viruses and protists associated with grossly normal specimens of three asteroid species (Patiriella regularis, Stichaster australis, Coscinasterias muricata) on the North Island / Te Ika-a-Māui, Aotearoa New Zealand, using metagenomes prepared from virus and ribosome-sized material. We discovered several densovirus-like genome fragments in our RNA and DNA metagenomic libraries. Subsequent survey of their prevalence within populations by quantitative PCR (qPCR) demonstrated their occurrence in only a few (13%) specimens (n = 36). Survey of large and small subunit rRNAs in metagenomes revealed the presence of a mesomycete (most closely matching Ichthyosporea sp.). Survey of large subunit prevalence and load by qPCR revealed that it is widely detectable (80%) and present predominately in body wall tissues across all 3 species of asteroid. Our results raise interesting questions about the roles of these microbiome constituents in host ecology and pathogenesis under changing ocean conditions.

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Myxospore density of Kudoa inornata varies significantly within symmetrical white muscle tissue replicates of its fish host, the spotted seatrout, Cynoscion nebulosus

Snyder,

McElroy,

de Buron

et al. 2024

Parasitol Res

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The spotted seatrout, Cynoscion nebulosus, is a popular game fish in the southeastern USA. It is estimated that nearly 90% of the adult population in South Carolina estuaries are infected in their skeletal muscle by the myxosporean, Kudoa inornata. However, little is known about this parasite’s biology, including the distribution and densities of myxospores within tissues of infected fish, which we expect affect the physiology of their hosts. In order to correlate densities with physiological parameters in future studies, we quantified the myxospores density in muscle and characterized the variation among individual fish. Naïve juvenile seatrout was experimentally infected via presumed K. inornata actinospores exposure to raw seawater. A plug of muscle was extracted from two bilaterally symmetrical regions in the epaxial fillet from fresh and frozen carcasses. Variation in density data was calculated both within and among individuals. Within individuals, density counts were compared between left- and right-side biopsies. There was no significant difference between fresh and frozen plugs, and variation among individuals accounted for the greatest proportion of variation at 68.8%, while variation within individuals was substantial at 25.6%. Simulation and correlation tests confirmed that bilaterally symmetrical replicates varied significantly within individuals. When sampled from areas surrounding the initial biopsies, myxospore density estimates were more similar than between sides. Our findings have important implications for sampling design, particularly for studies investigating physiological parameters at the cellular or molecular level in association with parasite infection.

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Pathogenic endoparasites of the spotted seatrout, Cynoscion nebulosus: patterns of infection in estuaries of South Carolina, USA

Cited by 9 publications

References 144 publications

Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three New Zealand asteroid species

Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three New Zealand asteroid species

Surveillance of densoviruses and mesomycetozoans inhabiting grossly normal tissues of three Aotearoa New Zealand asteroid species

Myxospore density of Kudoa inornata varies significantly within symmetrical white muscle tissue replicates of its fish host, the spotted seatrout, Cynoscion nebulosus

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