“…Several long-term studies performed on intact natural ecosystems such as Yellowstone National Park and Sierra Nevada of California in United States (Noss et al, 2002;Vredenburg et al, 2007), Eungella National Park in Australia (McDonald, 1990), and Monteverde Cloud Forest Preserve in Costa Rica (Pounds et al, 1997) show a worldwide decline in amphibian species in the last two decades. Populations of many species of frogs have declined dramatically in relatively undisturbed habitats at high altitudes and anthropized areas throughout the world (Blaustein & Wake, 1990, 1995Blaustein et al, 1994;Bradford, 1991;Campbell, 1999;Carey, 1993;Collins & Storfer 2003;Crump et al, 1992;Czechura & Ingram, 1990;Hero et al, 2005;Kiesecker et al, 2001;McDonald, 1990;McMenamin et al, 2008;Pounds, 2001;Reading, 2007;Richards et al, 1993;Skerratt et al, 2007;Stuart et al, 2004;Young et al, 2001). A map produced by IUCN shows the global distribution of threatened amphibians (Figure 2) revealing that the greatest concentration of threatened amphibian are in relatively limited areas dominated by species living within specific ranges, often living in mountainous areas.…”