Pathogenic Escherichia coli biosensor detection on chicken food samples

Abstract

Escherichia coli is an important pollution indicator and is the most important foodborne pathogens of public health concern. The chicken meat is one of the most important sources of good quality protein, and it is also susceptible to microbial contamination and often implicated in foodborne disease. In such context, the detection of E. coli K12 in frozen chicken meat was investigated by electrochemical impedance spectroscopy and surface plasmon resonance imaging techniques. The anti‐E. coli antibody was first immobilized onto gold surface by physisorption technique. The electrical and optical properties of the immobilized anti‐E. coli K12 antibody were studied. The binding of the E. coli K12 bacteria with the anti‐E. coli antibody layer was measured with a detection limit of 103 cfu/ml. This detection limit is better than those obtained with the enzyme‐linked immunosorbent assay (ELISA) technique. Moreover, the developed biosensor was used for E. coli K12 detection in inoculated frozen chicken meat. Practical applications Escherichia coli is an important pollution indicator and is the most important foodborne pathogens of public health concern. The present study deals with the development of bacteria biosensors based on electrochemical impedance spectroscopy and surface plasmon resonance imaging techniques for E. coli K12 detection in inoculated frozen chicken meat. A detection limit of 103 cfu/ml was obtained with the developed biosensors that was better than those obtained with classical methods such as ELISA (enzyme‐linked immunosorbent assay). This research work opens tremendous potential applications for the detection of pathogens in food at the early stage particularly with the development of miniaturized multiarrays platform based on impedance spectroscopy.

Sensitive Bacterial Detection via Dielectrophoretic-Enhanced Mass Transport Using Surface-Plasmon-Resonance Biosensors

“…The secondary-amplification demonstrates that dilute (10 3 CFU/mL) suspensions of target bacteria can be selectively detected in the presence of nontarget bacteria with iSPR chips. Multifunctional coatings, that incorporate nonfouling elements and highly selective biorecognition elements have enabled bacterial detection in complex matrices. − Synergistic effects between multifunctional coatings and the DEP-enhanced approach presented here could lead to further improvements in SPR-based bacterial detection.…”

Detection of Bacterial Pathogens and Antibiotic Residues in Chicken Meat: A Review

“…This colourimetric-based aptasensor has a high specificity towards viable cells of C. coli and C. jejuni. In another study, the electrochemical impedance spectroscopy technique was used to check the presence of E. coli K12 in a meat sample of frozen chicken [ 124 ]. For this, antibodies synthesized against E. coli were immobilized on to the gold surface via a physisorption method.…”

Fast Detection of Pathogenic Escherichia coli from Chicken Meats

“…With this aim, two samples of fresh chicken meat were kept in freezers at −18°C during 45 days [27]. The first sample (S1) was inoculated with E. coli with a concentration of 10 5 CFU/ML in PBS, and the second sample (S2) was kept in PBS buffer (reference).…”