Pathogenic mechanism of Eimeria tenella autophagy activation of chicken embryo cecal epithelial cells induced by Eimeria tenella

Duan, Buting; Zhao, Yangyang; Cui, Kai-ling; Xu, Tong; Lv, Xiaoling; Guo, Lulu; Zheng, Mingxue; Bai, Rui

doi:10.1016/j.psj.2023.102535

Cited by 4 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coccidiosis is characterized by intestinal injury, diarrhea, or bloody stools [5]. Eimeria tenella is the most virulent, widely distributed, and harmful of the seven chicken coccidia species, mainly attacking the cecum [6]. The highest incidence rate was found in 3~6-week-old chicks.…”

Section: Introductionmentioning

confidence: 99%

The Immunoprotective Effect of ROP27 Protein of Eimeria tenella

Li,

Lv,

Zheng

et al. 2023

Animals

View full text Add to dashboard Cite

Eimeria tenella rhoptry protein has the properties of a protective antigen. EtROP27 is a pathogenic gene that is detected via a transcriptome, but its expression pattern, immunogenicity, and potency are unknown. Therefore, a gene segment of EtROP27 was amplified and transplanted into the pET28a prokaryotic vector for the expression of the recombinant protein, and it subsequently purified for the generation of a polyclonal antibody. Then, RT-PCR and Western blotting were performed to understand the expression pattern of EtROP27. Subsequently, animal experiments were conducted to evaluate the immunoprotective effect of the recombinant protein with different immunizing doses (50, 100, and 150 μg). The results showed that the expression of EtROP27 gradually increased with the prolongation of infection time, reaching the highest level at 96 h and then decreasing. Additionally, EtROP27 is a natural antigen of coccidia that can stimulate the body to produce high levels of IgY. As with recombinant protein vaccines, the results of immune protection evaluation tests showed that the average weight gain rates of the immune challenge groups were significantly higher than that of the challenged control group, and their average lesion scores were significantly lower than that of the challenged control group. Furthermore, the oocyst excretion decreased by 81.25%, 86.21%, and 80.01%, and the anticoccidial index was 159.45, 171.47, and 166.75, respectively, for these groups. EtROP27 is a promising antigen gene candidate for the development of a coccidiosis vaccine.

show abstract

Section: Introductionmentioning

confidence: 99%

The Immunoprotective Effect of ROP27 Protein of Eimeria tenella

Li,

Lv,

Zheng

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

Plants, plant‐derived compounds, probiotics, and postbiotics as green agents to fight against poultry coccidiosis: A review

Chen,

Liu,

Yue

et al. 2024

Animal Research and One Health

View full text Add to dashboard Cite

The intestinal tract is the main place for animals to digest food and absorb nutrients, which also serves as the first line of defense against pathogens that invade the internal environment. Therefore, normal intestinal structure and function are essential for animal health. Poultry coccidiosis is an intestinal disease primarily caused by the parasitization of intestinal epithelial cells by protozoa of the genus Eimeria. The occurrence of coccidiosis not only compromises the intestinal integrity of poultry but also increases their disease susceptibility, thus posing a serious threat to the overall health and productivity of poultry. Nowadays, the primary methods for controlling and preventing coccidiosis in poultry are anticoccidial drugs or live oocyst vaccines. However, the use of the former may be associated with problems of resistance and drug residues, while the use of the latter may cause intestinal damage and significantly increase farming costs. For these reasons, it is critical to investigate green, safe, and cost‐effective natural alternative strategies such as phytochemicals and probiotics for controlling coccidiosis as well as mitigating the deleterious effects of coccidial infections in production. In this review, we aim to summarize the role, mechanisms, and therapeutic potential of natural products in the treatment of coccidiosis to lay a theoretical foundation for effective coccidiosis control.

show abstract