Pathogenic potential to humans of Shiga toxin-producing Escherichia coli isolated from wild boars in Poland

Szczerba‐Turek, Anna; Socha, Piotr; Bancerz‐Kisiel, Agata; Platt-Samoraj, A.; Lipczyńska-Ilczuk, Karolina; Siemionek, J.; Kończyk, K.; Terech-Majewska, E.; Szweda, W.

doi:10.1016/j.ijfoodmicro.2019.04.004

Cited by 15 publications

(12 citation statements)

References 35 publications

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“…However, some studies showed circulation of this pathotype in wild animals too [10]. In particular, concerning wild boar, our results are not dissimilar from those of other studies carried out in Europe, where the percentage of positive animals ranged between 3% and 30% [17][18][19][20][21][22]. In the present study, the most detected gene coding for Shiga-like toxins was stx2.…”

Section: Discussioncontrasting

confidence: 58%

“…More recently, a study conducted in Portugal showed 4.8% positivity for STEC in wild boar [20]. In Poland, 28.2% and 30.9% STEC and EPEC, respectively, were isolated from rectal swabs of wild boar [21]. In Italy, a recent investigation carried out on different wild animals identified one EPEC and one EHEC isolate from a small number of wild boar [22].…”

Section: Introductionmentioning

confidence: 99%

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Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany

Bertelloni

Cilia

Bogi

et al. 2020

Animals

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Wild boar are among the most widespread wild mammals in Europe. Although this species can act as a reservoir for different pathogens, data about its role as a carrier of pathogenic and antimicrobial-resistant Escherichia coli are still scarce. The aim of this study was to evaluate the occurrence of antimicrobial-resistant and pathogenic Escherichia coli in wild boar in the Tuscany region of Italy. During the hunting season of 2018–2019, E. coli was isolated from 175 of 200 animals and subjected to antimicrobial resistance tests and PCR for detection of resistance and virulence factor genes. The highest resistance rates were against cephalothin (94.3%), amoxicillin–clavulanic acid (87.4%), ampicillin (68.6%), and tetracycline (44.6%). The most detected resistance genes were blaCMY-2 (54.3%), sul1 (38.9%), sul2 (30.9%), and tetG (24.6%). Concerning genes encoding virulence factors, 55 of 175 isolates (31.4%) were negative for all tested genes. The most detected genes were hlyA (47.4%), astA (29.1%), stx2 (24.6%), eaeA (17.1%), and stx1 (11.4%). E. coli was classified as Shiga toxin-producing E. coli (STEC) (21.7%), enterohemorrhagic E. coli (EHEC) (6.3%), enteroaggregative E. coli (EAEC) (5.1%), and atypical enteropathogenic E. coli (aEPEC) (3.4%). Enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and typical enteropathogenic E. coli (tEPEC) were not detected. Our results show that wild boars could carry pathogenic and antimicrobial-resistant E. coli, representing a possible reservoir of domestic animal and human pathogens.

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Section: Discussioncontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany

Bertelloni

Cilia

Bogi

et al. 2020

Animals

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“…The gene eae responsible for the typical attaching and effacing lesions was used as a target for the detection of Enteropathogenic E. coli (EPEC), which are defined as intimin (eae)-containing diarrheagenic E. coli that do not possess the stx genes (Alonso et al, 2017). Wild boars acting as a carrier of EPEC have been previously demonstrated by Szczerba-Turek et al, (2019) who detected them in 30.9% of the animals and other Spanish and Italian studies but a lower occurrence was reported (Spain = 3.3%, Alonso et al, 2017;Italy = 3.4%, Bertelloni et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Presence of enteric bacterial pathogens in meat samples of wild boar hunted in Campania region, southern Italy

et al. 2022

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Wild boars can be infected with several foodborne pathogens which may be transmitted to humans through the consumption of their meat, but currently, data of their prevalence are still limited. The present study aimed to evaluate the presence of enteric pathogens in wild boar meat samples killed in the Campania region. Twentyeight wild boar meat samples were analyzed for the detection of Salmonella spp, Y. enterocolitica, Campylobacter spp., and Shiga- Toxigenic E. coli. Salmonella spp. was detected and isolated in ten samples and after serotyping S. Veneziana, S. Kasenyi, S. Coeln, S. Manhattan, S. Thompson, and S. Stanleyville were identified. Twenty-one meat samples were found to be contaminated with Y. enterocolitica; in 6 samples the ystA and ystB genes were detected simultaneously, while in 15 only the ystB gene, which characterizes the bacteria belonging to the biotype 1A, was present. Shiga-Toxin producing E. coli was detected in 12 while Campylobacter spp was never detected. In conclusion, due to the high occurrence of pathogenic bacteria detected, the present research shows that wild boars are important reservoirs for foodborne zoonoses which may be transmitted to livestock and humans. This confirms the importance of controls throughout the wild boar supply chain. In the Campania region, checks are guaranteed by the Veterinarians who work within the “management and control plan for wild boar in the Campania region” which has the twofold objective of containing the increasingly invasive presence of this animal and guaranteeing greater safety, traceability, and transparency in the consumption of meat.

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“…The serogrouping of the Stx2e-producing E. coli isolated from clinical cases of colibacillosis was based on somatic O-antigens since, among them, there are the most frequent and pathogenic for humans and animals (17). The analyses was carried out using agglutinating antisera in microplate according to Guinée et al (18) and modified by Blanco et al (19).…”

Section: Serogrouping Of the Isolatesmentioning

confidence: 99%

Pathogenicity of Shiga Toxin Type 2e Escherichia coli in Pig Colibacillosis

et al. 2020

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Shiga toxin type 2e (Stx2e) Escherichia coli is the causative factor of diarrhea and edema in swine. The aims of this study were to determine the prevalence of Stx2e-producing E. coli isolates and to characterize isolates from clinical cases of pig colibacillosis and healthy swine. During the 11 years of the study (2006-2017), a total of 233 Stx2e-producing isolates were detected−230 out of 2,060 (11.16%) E. coli isolated from diseased pigs and 3 out of 171 (1.75%) from healthy swine. Stx2e-producing isolates were indeed more present in clinical colibacillosis cases than in healthy pigs (p = 0.0002). The predominant serogroup was O139 (79.82%) and the most common fimbrial factor present in these isolates was F18 (177 isolates), followed by F6 (5 isolates). The enterotoxins LTI, STa, and STb were detected in 10.43, 41.73, and 48.26% of the isolates, respectively. The predominant virotypes F18-Stx2e and-STa-STb-Stx2e were similarly present in weaners (33.33 and 35.52%) and finishers (38.30 and 25.53%). Among isolates from diseased pigs, O139 and F18 were the more frequently identified serogroup and virulence factor, respectively. Of the tested 230 Stx2e-producing isolates isolated from diseased pigs, 29 (12.60%) harbored genes encoding ESBL, particularly TEM (79.30%), CTX-M1 (17.20%), and CMY-2 (3.40%). Antimicrobial resistance to tetracycline was the most common characteristic (98.25%), followed by ampicillin (93.91%), cephalotin (90.43%) and trimethoprim/sulfamethoxazole (82.17%). Our results showed that Stx2e-producing E. coli were more frequently associated with clinical forms of colibacillosis, with minimal probability to isolate these isolates from healthy pigs.

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Pathogenic potential to humans of Shiga toxin-producing Escherichia coli isolated from wild boars in Poland

Cited by 15 publications

References 35 publications

Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany

Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany

Presence of enteric bacterial pathogens in meat samples of wild boar hunted in Campania region, southern Italy

Pathogenicity of Shiga Toxin Type 2e Escherichia coli in Pig Colibacillosis

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