Pathogenic variability in<i>Mycosphaerella fijiensis</i>Morelet, cause of black Sigatoka in banana and plantain

Fullerton, R. A.; Olsen, T. L.

doi:10.1080/01140671.1995.9513866

Cited by 43 publications

(47 citation statements)

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“…Grosh Michel a susceptible (AAA genome group) provided reproducible and reliable results for the level of virulence of isolates Mak01, Mak02 and Kaw10. Symptom development for these isolates corresponded closely with the descriptions of Fullerton and Olsen (1995) for in vitro plants (cv. Grande Naine), inoculated with conidia.…”

Section: Discussionsupporting

confidence: 71%

“…Grande Naine), inoculated with conidia. Our results are encouraging, given that Fullerton and Olsen (1995) used significantly different inoculum and inoculation conditions than what was used in our study, and yet M. fijiensis disease development on the two susceptible cultivars, (cv. Gross Michel and cv.…”

Section: Discussionmentioning

confidence: 53%

“…The method reported is the first to validate the reliability of M. fijiensis virulence assays under ordinary screenhouse conditions (Fullerton and Olsen, 1995) (room temperature, average of ambient temperatures of 28 o C) in which myccelia fragmented with a blender and weighed, were used as inoculum for artificial inoculation, as opposed to mycelia fragmented by bead beating and quantified using a heamocytometer.…”

Section: Discussionmentioning

confidence: 99%

“…After 48 hr, plants were removed and placed in a screenhouse, and examined weekly for the first two weeks, and subsequently daily for symptoms on both leaf surfaces. A 1-5 scale (Table 2) developed by Fullerton and Olsen (1995) was used to evaluate Experimental design and data analysis. All experiments were established following a randomised complete block design, with four replications and repeated at least once.…”

Section: Methodsmentioning

confidence: 99%

“…The number of lesions was used to calculate Areas Under Disease Progress Curve (AUDPC) (Madden et al, 2007) prior to statistical analysis. All 0-5 stage of black sigatoka disease symptom development as described by Fullerton and Olsen (1995) ( Capo et al, 2003) A B C D E Figure 1. Black Sigatoka lesion types on AAA-EA genome (A=Mbwazirume, B=Mpologoma, C=Musakala, D=Nfuuka) and E=M9 Hybrid (AAAA).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Efficient screening procedure for black sigatoka disease of banana

Kumakech¹,

Jørgensen²,

Edema³

et al. 2015

Afr. Crop Sci. J.

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Current methods for screening for reaction of Mycosphaerella fijiensis are mostly field based and subject to variability caused by weather conditions. The objective of this study was to develop an efficient screening procedure for black sigatoka disease in order to provide a reliable controlled environment screening method. A fragmented mycelia-based infection system was developed using weighed mycelia suspensions, in 0.18% water agar. Consistent and repeatable black sigatoka infection was achieved in cv. Gross Michel as a reference susceptible cultivar. The infection system developed was used to validate screenhouse reaction of banana genotypes to M. fijiensis. Three East African highland genotypes (AAA-EA), one plantain genotype (ABB), two dessert (AAB and AAA) and one hybrid genotype (AAAA) were tested. Inoculum of M. fijiensis was adjusted to 15 mg ml -1 fragmented mycelium and applied to the abaxial surfaces of the first two open leaves. Plants were evaluated for 6 weeks post inoculation (PI). Significant differences in the response of the different genotypes, to M. fijiensis infection were observed at 3, 4, 5 and 6 weeks PI. All East African highland and dessert genotypes were susceptible. M9 hybrid and Psang awak (ABB) expressed resistance, characterised by high disease incubation period and slow rate of symptom development, with Psanag awak being the most resistant. Artificial inoculation of in vitro plants with weighed fragmented mycelial suspension, was practicable for determining resistance to M. fijiensis. This is the first report demonstrating that use of weighed fragmented mycelial inoculum spread over the entire leaf surface, can be used to quantitatively assess reaction of banana genotypes to M. fijiensis under screenhouse conditions. Key Words: Ascomycete, Black leaf streak, Mycosphaerella fijiensis Musa sp. RÉSUMÉLes méthodes actuelles de dépistage de Mycosphaerella fijiensis sont essentiellement basées sur des évaluations dans le champ et sont souvent sujets à des variabilités climatiques. L'objectif de cette étude était de développer une procédure efficace de dépistage de la maladie foliaire du bananier Sigatoka dans le but de proposer une méthode de dépistage non influencée par l'environnement. Un système d'infection a été développé en se servant de mycélium en suspension dans 0,18% de solution agar. Des infections consistantes et répétées ont été réalisées in cv. La variété Gross Michel a été utilisée comme référence susceptible. Le système d'infection développé a été utilisé pour valider la réaction des accessions de bananiers en serre à M. fijiensis. Trois génotypes de bananiers adaptés aux hautes altitudes en Afrique de l'Est (AAA-EA), un génotype de type plantain (ABB), deux déserts (AAB and AAA) et un hybride (AAAA) ont été testés. Des inocula de M. fijiensis ont été doses à 15 mg ml-1 et appliqués sur la face dorsale des deux premières feuilles ouvertes. Les plants ont été évalués pendant 6 semaines après inoculation (PI). Des différences significatives ont été observées dans ...

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Efficient screening procedure for black sigatoka disease of banana

Kumakech¹,

Jørgensen²,

Edema³

et al. 2015

Afr. Crop Sci. J.

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show abstract

Construction and characterization of a plant transformation-competent BIBAC library of the black Sigatoka-resistant banana Musa acuminata cv. Tuu Gia (AA)

et al. 2005

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A plant transformation-competent binary bacterial artificial chromosome (BIBAC) library was constructed from Musa acuminata cv. Tuu Gia (AA), a black Sigatoka-resistant diploid banana. After digestion of high-molecular-weight banana DNA by HindIII, several methods of DNA size selection were tested, followed by ligation, using a vector/insert molar ratio of 4:1. The library consists of 30,700 clones stored in 80 384-well microtiter plates. The mean insert size was estimated to be 100 kb, and the frequency of inserts with internal NotI sites was 61%. The majority of insert sizes fell into the range of 100+/-20 kb, making them suitable for Agrobacterium-mediated transformation. Only 1% and 0.9% of the clones contain chloroplast and mitochondrial DNA, respectively. This is the first BIBAC library for banana, estimated to represent five times its haploid genome (600 Mbp). It was demonstrated by hybridization that the library contains typical members of resistance gene and defense gene families that can be used for transformation of disease susceptible banana cultivars for banana genetic improvement.

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Construction of a genetic linkage map of the fungal pathogen of banana Mycosphaerella fijiensis, causal agent of black leaf streak disease

et al. 2008

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A genetic linkage map of the fungal plant pathogen Mycosphaerella fijiensis, causal agent of black leaf streak disease of banana was developed. A cross between the isolates CIRAD86 (from Cameroon) and CIRAD139A (from Colombia) was analyzed using molecular markers and the MAT locus. The genetic linkage map consists of 298 AFLP and 16 SSR markers with 23 linkage groups, containing five or more markers, covering 1,879 cM. Markers are separated on average by around 5.9 cM. The MAT locus was shown to segregate in a 1:1 ratio but could not be successfully mapped. An estimate of the relation between physical size and genetic distance was approximately 39.0 kb/cM. The estimated total haploid genome size was calculated using the genetic mapping data at 4,298.2 cM. This is the first genetic linkage map reported for this important foliar pathogen of banana. The great utility of the map will be for anchoring contigs in the genome sequence, evolutionary studies in comparison with other fungi, to identify quantitative trait loci (QTLs) associated with aggressiveness or oxidative stress resistance and with the recently available genome sequence, for positional cloning.

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Pathogenic variability inMycosphaerella fijiensisMorelet, cause of black Sigatoka in banana and plantain

Cited by 43 publications

References 0 publications

Efficient screening procedure for black sigatoka disease of banana

Efficient screening procedure for black sigatoka disease of banana

Construction and characterization of a plant transformation-competent BIBAC library of the black Sigatoka-resistant banana Musa acuminata cv. Tuu Gia (AA)

Construction of a genetic linkage map of the fungal pathogen of banana Mycosphaerella fijiensis, causal agent of black leaf streak disease

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