Pathogenic Vibrio, Aeromonas and Arcobacter spp. associated with copepods in the Straits of Messina (Italy)

Gugliandolo, Concetta; Irrera, G.P.; Lentini, Valeria; Maugeri, Teresa L.

doi:10.1016/j.marpolbul.2007.12.001

Cited by 56 publications

(43 citation statements)

References 30 publications

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“…Copepods have previously been shown to host some Vibrio spp. (Huq et al ., 1983; Tamplin et al ., 1990; Heidelberg et al ., 2002; Gugliandolo et al ., 2008; Vezzulli et al ., 2015); however, some Vibrio spp. seem to be unable to colonize some copepod species (Dumontet et al ., 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Live feed used in aquaculture is a potential point of entry and vector for pathogenic bacteria (Olafsen, 2001), and several Vibrio species, including the human and fish pathogens V. cholerae, V. vulnificus and V. splendidus, are found in association with zooplankton, for example copepods, which in the marine environment seem to serve as natural Vibrio reservoirs (Sochard et al ., 1979; Heidelberg et al ., 2002; Colwell et al ., 2003; Vezzulli et al ., 2015). The role of copepods as hosts for V. cholerae has especially been studied extensively, and V. cholerae has repeatedly been isolated from the surface, gut and exuviae of these small crustaceans (Huq et al ., 1983; Tamplin et al ., 1990; Gugliandolo et al ., 2008). Thus, although vibrios such as Vibrio parahaemolyticus , V. alginolyticus and V. mimicus have been reported to be unable to colonize the copepod species Temora stylifera , Acartia clausi , Centropages typicus and Paracalanus parvus (Dumontet et al ., 1996), it is a valid concern that Acartia tonsa may function as infection vectors for other Vibrio species including the fish pathogenic V. anguillarum in aquaculture systems.…”

Section: Introductionmentioning

confidence: 99%

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Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

Rasmussen

Erner

Bentzon-Tilia

et al. 2018

Microbial Biotechnology

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SummaryThe expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for example rotifers and Artemia as well as larvae of turbot and cod against pathogenic vibrios. Here, we show that the emerging live feed, copepods, is unaffected by colonization of the fish pathogen Vibrio anguillarum, making them potential infection vectors. However, TDA‐producing Phaeobacter inhibens was able to significantly inhibit V. anguillarum in non‐axenic cultures of copepod Acartia tonsa and the copepod feed Rhodomonas salina. Vibrio grew to 106 CFU ml−1 and 107 CFU ml−1 in copepod and R. salina cultures, respectively. However, vibrio counts remained at the inoculum level (104 CFU ml−1) when P. inhibens was also added. We further developed a semi‐strain‐specific qPCR for V. anguillarum to detect and quantify the pathogen in non‐axenic systems. In conclusion, P. inhibens efficiently inhibits the fish larval pathogen V. anguillarum in the emerging live feed, copepods, supporting its use as a probiotic in aquaculture. Furthermore, qPCR provides an effective method for detecting vibrio pathogens in complex non‐axenic live feed systems.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

Rasmussen

Erner

Bentzon-Tilia

et al. 2018

Microbial Biotechnology

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“…Mostly abundant in corals, oysters, turbot larval gut, Haemolytic, septicaemic, necrotic Sugumar et al (1998), Thompson et al (2003), Koren and Rosenberg (2006) Arcobacter butzleri Brackish marine environment Human and animal pathogen, secretion of toxic factors Carbone et al (2003), Gugliandolo et al (2007) Pseudoalteromonas group #1 and Vibrio group #4 contain closely related pathogenic and non-pathogenic species structure and activities at cellular level are well documented (Thuesen and Kogure 1989;Do et al 1990;Ritchie et al 2000). TTX-carrying animals are known from different marine, limnic and terrestrial habitats of warmer regions.…”

Section: Discussionmentioning

confidence: 99%

“…Jellyfish Cyanea lamarckii provides the natural habitat for Moritella viscosa, which causes necrotic ''winter ulcer'' in salmons (Lunder et al 2000;Heidarsdottir et al 2008), additionally Arcobacter butzleri, a human pathogen species (Carbone et al 2003;Gugliandolo et al 2007), and finally members of group #4 (see the previous paragraph). The Lions mane jellyfish C. capillata carries the haemolytic species Shewanella waksmanii, a member of group #3 (Ivanova et al 2003), and pathogenic endobionts of the already described Photobacterium profundum and the putatively pathogenic species of groups #1 and #4 (see the previous paragraph).…”

Section: Pathogenic Activities Of Bacterial Endobiontsmentioning

confidence: 99%

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Schuett

Doepke

2009

Helgol Mar Res

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Endobiotic bacteria colonize the tentacles of cnidaria. This paper provides first insight into the bacterial spectrum and its potential of pathogenic activities inside four cnidarian species. Sample material originating from Scottish waters comprises the jellyfish species Cyanea capillata and C. lamarckii, hydrozoa Tubularia indivisa and sea anemone Sagartia elegans. Mixed cultures of endobiotic bacteria, pure cultures selected on basis of haemolysis, but also lyophilized samples were prepared from tentacles and used for DGGE-profiling with subsequent phylogenetic analysis of 16S rDNA fragments. Bacteria were detected in each of the cnidarian species tested. Twenty-one bacterial species including four groups of closely related organisms were found in culture material. The species within these groups could not be differentiated from each other (one group of Pseudoalteromonas spp., two groups of Shewanella spp., one group of Vibrio spp.). Each of the hosts exhibits a specific endobacterial spectrum. Solely Cyanea lamarckii harboured Moritella viscosa. Only in Cyanea capillata, members of the Shewanella group #2 and the species Pseudoalteromonas arctica, Shewanella violacea, Sulfitobacter pontiacus and Arcobacter butzleri were detected. Hydrozoa Tubularia indivisa provided an amazingly wide spectrum of nine bacterial species. Exclusively, in the sea anemone Sagartia elegans, the bacterial species P. aliena was found. Overall eleven bacterial species detected were described recently as novel species. Four 16S rDNA fragments generated from lyophilized material displayed extremely low relationship to their next neighbours. These organisms are regarded as members of the endobiotic ''terra incognita''. Since the origin of cnidarian toxins is unclear, the possible pathogenic activity of endobiotic bacteria has to be taken into account. Literature data show that their next neighbours display an interesting diversity of haemolytic, septicaemic and necrotic actions including the production of cytotoxins, tetrodotoxin and R-toxin. Findings of haemolysis tests support the literature data. The potential producers are Endozoicimonas elysicola, Moritella viscosa, Photobacterium profundum, P. aliena, P. tetraodonis, Shewanella waksmanii, Vibrio splendidus, V. aestuarius, Arcobacter butzleri.

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“…The virulence of A. cryaerophilus was also first described when it was observed that the strains tested induced the accumulation of fluid and electrolytes in the rat ileal loop assay and showed an in vitro invasion of Hep-2 cells (7). Several studies were reported on their adhesion capacity (8)(9)(10), invasiveness (9,11), and cytotoxicity (12)(13)(14) in several cell lines. Collectively, in these studies, the strains tested showed adhesion, invasion, and cytotoxicity, toxicity and adherence being the most commonly observed effects.…”

Section: Introductionmentioning

confidence: 99%

Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri

Adesiji¹,

Emikpe²,

Opalekunde³

2011

AML

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Background.Arcobacter is an emerging zoonotic enteropathogen associated with spontaneous abortion, diarrhea and mastitis in domestic animals.Materials and methods. Thirty male pathogen-free albino rats were infected with a single challenge of Arcobacter butzleri (10 8 cfu/ml) isolated from the stool of healthy pigs with the aim of investigating the effects of A. butzleri on rat testicular histology and spermatogenesis.Results. In previously healthy male albino rats, A. butzleri caused testicular degeneration associated with reduced sperm count and motility.Conclusions. The result of this study suggests that A. butzleri produces testicular degeneration and the associated disruption of spermatogenesis in albino rats; hence, its infertility potential in livestock industry and its economic importance should be further investigated. Key words:Arcobacter butzleri, testicular degeneration, infertility,pathogenicity, sperm count, albino rats INTRODUCTIONArcobacter is an emerging enteropathogen with an increasing evidence of the zoonotic potential associated with prolonged diarrhoea and occasional systemic infections such as bactereamia and peritonitis in humans (1). An evidencebased semi-quantitative method for prioritization of foodborne zoonoses ranked A. butzleri as a microbe of "significant importance" (2). Arcobacter was first isolated from aborted bovine and porcine fetuses (3), especially A. butzleri and A. skirrowii which were isolated from aborted fetuses and placenta of bovine, porcine and ovine origin (4). Although no remarkable pathological changes were identified, the clinical signs associated with Arcobacter infection include infertility, chronic discharge during oestrus, stillbirth and late-term abortions (5).The pathogenicity and virulence mechanisms of Arcobacter species are still poorly understood, despite its increasing incidence and isolation from clinical and food products (6). The virulence of A. cryaerophilus was also first described when it was observed that the strains tested induced the accumulation of fluid and electrolytes in the rat ileal loop assay and showed an in vitro invasion of Hep-2 128Yemisi Olukemi Adesiji, Benjamin Obukowho Emikpe, Ahmed Bayo Opalekunde cells (7). Several studies were reported on their adhesion capacity (8-10), invasiveness (9, 11), and cytotoxicity (12)(13)(14) in several cell lines. Collectively, in these studies, the strains tested showed adhesion, invasion, and cytotoxicity, toxicity and adherence being the most commonly observed effects. The differences observed among different studies may be due to the origin of the strains (environmental versus clinical) as well as to different cell lines used in the studies, A. butzleri being the most invasive species in experimental animal infections (15). Other animal models and in vivo experiments have been reviewed in another recent publication (16). The presence of adhesion molecules in A. butzleri have been proven by the capacity of the strains tested to agglutinate human, rabbit, and sheep erythrocytes, and hemagglut...

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Pathogenic Vibrio, Aeromonas and Arcobacter spp. associated with copepods in the Straits of Messina (Italy)

Cited by 56 publications

References 30 publications

Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

Endobiotic bacteria and their pathogenic potential in cnidarian tentacles

Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri

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