Pathogenicity and epidemiological survey of fowl adenovirus in Shandong Province from 2021 to 2022

Wang, Tailong; Meng, Fanliang; Chen, Changxiu; Shen, Yesheng; Li, Peixun; Xu, Jiamin; Feng, Zhaomin; Qu, Xiangjin; Wang, Fuyong; Li, Baoquan; Liu, Mengda

doi:10.3389/fmicb.2023.1166078

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…FAdV-4, which mainly infects 3- to 5-week-old broilers [ 16 , 17 ], was first reported in Pakistan and later spread to other countries [ 18 ]. Since 2015, a novel highly pathogenic FAdV-4 has been spreading in many provinces of China, causing huge economic losses to the poultry industry [ 19 , 20 , 21 , 22 , 23 ]. The latest research shows that FAdV-4 could be isolated from wild birds and infect domestic mallard ducks, which has brought new threats to the poultry industry [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

The Development of a Novel Fiber-2 Subunit Vaccine against Fowl Adenovirus Serotype 4 Formulated with Oil Adjuvants

Liu,

Wang

et al. 2024

Vaccines

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Hepatitis-hydropericardium syndrome (HHS), caused by fowl adenovirus serotype 4 (FAdV-4), has been widely spread across China, resulting in great financial losses in the poultry industry. Therefore, efficient vaccines against this disease urgently need to be developed. In our study, the fiber-2 and penton base proteins derived from the FAdV-4 JS strain were expressed in a prokaryotic system (E. coli) in a soluble form. Then, the efficacy of the two recombinant proteins formulated with cheap and widely used adjuvants (Marcol™ 52 white oil) were respectively tested, and the minimum immune doses and safety of the above proteins were also determined. It was indicated that the fiber-2 (20 µg/bird, 200 µg/bird) and penton base (200 µg/bird) could provide complete protection against the highly pathogenic FAdV-4 and suppress its replication and shedding. Unfortunately, only the fiber-2 protein could induce complete protection (10/10) at a low dose (10 µg/bird). In addition, we confirmed that the fiber-2 subunit vaccine formulated with oil adjuvants was safe for vaccinated chickens. Conclusively, all of our results suggest that we successfully prepared an efficient and cheap fiber-2 subunit vaccine with few side effects.

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Section: Introductionmentioning

confidence: 99%

The Development of a Novel Fiber-2 Subunit Vaccine against Fowl Adenovirus Serotype 4 Formulated with Oil Adjuvants

Liu,

Wang

et al. 2024

Vaccines

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“…Although commercial vaccination against FAdV-4 has been implemented, FAdV-4 infection still occurred sporadically. In most of the previous studies on the isolation and sequencing of FAdVs from commercial broiler and layer chickens [13][14][15], no systematic epidemiological investigation and study of the molecular characteristics of FAdVs from indigenous chickens has been performed.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characteristics of FAdV-4 from Indigenous Chicken Breeds in Yunnan Province, Southwestern China

Lai,

Yang,

Chen

et al. 2023

Microorganisms

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Fowl adenovirus-induced hepatitis–pericardial effusion syndrome outbreaks have been increasingly reported in China since 2015, resulting in substantial economic losses to the poultry industry. The genetic diversity of indigenous chicken results in different immune traits, affecting the evolution of these viruses. Although the molecular epidemiology of fowl adenovirus serotype 4 (FAdV-4) has been well studied in commercial broiler and layer chickens, the prevalence and genetic characteristics of FAdV-4 in indigenous chickens remain largely unknown. In this study, samples were collected from six indigenous chicken breeds in Yunnan province, China. FAdV-positive samples were identified in five of the six indigenous chicken populations via PCR and 10 isolates were obtained. All FAdVs belonged to serotype FAdV-4 and species FAdV-C. The hexon, fiber, and penton gene sequence comparison analysis demonstrated that the prevalence of FAdV-4 isolates in these chickens might have originated from other provinces that exported chicks and poultry products to Yunnan province. Moreover, several distinct amino acid mutations were firstly identified in the major structural proteins. Our findings highlighted the need to decrease inter-regional movements of live poultry to protect indigenous chicken genetic resources and that the immune traits of these indigenous chickens might result in new mutations of FAdV-4 strains.

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Pathological investigation, molecular characterization and first-time isolation of the predominant serotypes of fowl adenovirus (FAdV-D and E) from commercial poultry in Bangladesh

Sadekuzzaman,

Miah,

Parvin

et al. 2024

Front. Microbiol.

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BackgroundFowl adenovirus (FAdV) is a globally distributed virus that inflicts significant economic losses on the poultry industry. The study aimed at pathological investigation, molecular characterization, isolation, and pathogenicity determination of FAdV from commercial poultry.MethodsA total of 86 liver samples were collected from 80 commercial chicken farms. All samples were examined for gross pathology; only liver samples were used for histopathology and virus detection by PCR. PCR-positive FAdV samples were used for isolation of virus in 10-day-old seronegative embryonated chicken eggs (ECEs) via chorioallantoic membrane (CAM). PCR confirmed virus isolates were sequenced for serotyping and phylogenetic analysis. Pathogenicity of the isolated FAdVs was assessed by oral, i.m., and i.p. routes of infection.ResultsThe study observed gross lesions including hydropericardium and enlarged, friable pale livers with ecchymotic hemorrhages. Histopathological analysis revealed hepatocytic necrosis with basophilic intranuclear inclusion bodies in liver tissues, and tubular necrosis, focal hemorrhages, and mononuclear cell infiltration in kidney tissues. Out of 86 liver samples analyzed, 40 were positive for FAdVs by PCR, and 22 were positive for virus isolation. One serotype was 11 and other 11 were 8b of FAdV and genetically close to Bangladesh, India, and Turkey isolates, according to hexon gene phylogenetic analysis. The pathogenicity test indicated that serotype 11 was more virulent than the serotype 8b.ConclusionThe study concluded that serotypes 11 and 8b of FAdVs are circulating simultaneously among commercial broiler and layer chickens, serotype 8b was found predominant one.

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Pathogenicity and epidemiological survey of fowl adenovirus in Shandong Province from 2021 to 2022

Cited by 3 publications

References 37 publications

The Development of a Novel Fiber-2 Subunit Vaccine against Fowl Adenovirus Serotype 4 Formulated with Oil Adjuvants

The Development of a Novel Fiber-2 Subunit Vaccine against Fowl Adenovirus Serotype 4 Formulated with Oil Adjuvants

Prevalence and Molecular Characteristics of FAdV-4 from Indigenous Chicken Breeds in Yunnan Province, Southwestern China

Pathological investigation, molecular characterization and first-time isolation of the predominant serotypes of fowl adenovirus (FAdV-D and E) from commercial poultry in Bangladesh

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