Pathogenicity and fungicide sensitivity of Phytophthora parvispora, a new pathogen causing gummosis and root rot disease on citrus trees

Tran, Quang Van; Ha, Cuong Viet; Vvedensky, V. V.; Le, Thi Thuy Linh; Han, Viet‐Cuong

doi:10.1016/j.micpath.2023.105986

Cited by 7 publications

(17 citation statements)

References 19 publications

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“…Detailed information about the samples is shown in Tables S1. Phytophthora isolates were obtained from rhizosphere soil using the baiting technique or from diseased roots and stem according to the methods described by Tran et al (2023).…”

Section: Methodsmentioning

confidence: 99%

“…Molecular identification based on the internal transcribed spacer (ITS) and the cytochrome c oxidase subunit 1 mitochondrial ( cox 1) loci was performed following the standard procedure described by Abad et al (2022), which is available online at https://idtools.org/phytophthora/index.cfm. DNA was extracted using a NaOH method which was originally developed for plant tissue (Wang et al, 1993) with minor modifications, as described previously (Tran et al, 2023). The ITS region and cox 1 gene were amplified using ITS4/ITS5 primers (White et al, 1990) and OomCoxILevup/Fm85mod primers (Robideau et al, 2011), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…All pathogenicity tests were performed on mature fruit and stem of pomelo ( C. grandis ), orange ( C. sinensis ) and lime ( C. aurantiifolia ). Zoospores or mycelial plugs from 5‐day‐old cultures on V8 medium of four representative isolates, VN‐Oo38 ( P. palmivora ), VN‐Oo65 ( P. nicotianae ), VN‐Oo76 ( P. citrophthora ) and VN‐Oo68 ( P. mekongensis ), were used for inoculation according to the methods described by Tran et al (2023). In fruit inoculation, mature fruit after harvesting were surface‐disinfected with 70% ethanol and air‐dried in a laminar flow cabinet.…”

Section: Methodsmentioning

confidence: 99%

“…Six fungicides (Table 1 for the chemical formulations) were evaluated in vitro for their inhibitory efficacy against the growth of four representative Phytophthora isolates using a well‐diffusion method on a PDA (potato dextrose agar) Petri plate as previously described (Han et al, 2022; Tran et al, 2023). Three 5‐mm‐diameter mycelial plugs were placed at three positions with equal distances from each other on the perimeter of each plate.…”

Section: Methodsmentioning

confidence: 99%

“…A recent investigation into the diversity of Phytophthora in Vietnam suggested that the origin of most Phytophthora taxa, including P. cinnamomi and P. ramorum , can be traced back to Vietnam, and Phytophthora clades 2, 5, 6, 7, 8 and 9 are considered indigenous to Indochina (Jung et al, 2020, 2021). Another recent study identified P. parvispora , a member of clade 7, as a new pathogen causing gummosis and root rot in citrus trees in Vietnam (Tran et al, 2023). Thus, it is hypothesized that new Phytophthora species, in addition to the known species, may be involved in stem gummosis and root rot in citrus in Northern Vietnam.…”

Section: Introductionmentioning

confidence: 99%

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Current status and characterization of Phytophthora species associated with gummosis of citrus in Northern Vietnam

Tran

Vvedensky

et al. 2023

Journal of Phytopathology

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Phytophthora species have long been reported to cause Phytophthora disease in citrus with symptoms of gummosis, root rot and fruit brown rot. Due to the lack of an up‐to‐date investigation of Phytophthora species currently causing the disease in Northern Vietnam, this study aimed to determine the current Phytophthora species associated with the disease in this region. An extensive collection of diseased samples was conducted in the main citrus‐growing areas in Northern Vietnam from 2021 to 2022. Fifty‐four Phytophthora isolates were obtained and initially divided into four groups based on morphological characteristics, including colony morphology, mycelial growth rate and reproductive structures. Representative Phytophthora isolates from each group were then identified as P. palmivora, P. nicotianae, P. citrophthora and P. mekongensis based on the phylogenetic analysis of ITS and cox1 gene sequences. Pathogenicity tests showed that these four Phytophthora species induce gummosis and brown rot on pomelo, orange and lime, with pomelo being the most susceptible, followed by orange and lime, respectively. Koch's postulates were confirmed. All the Phytophthora species were sensitive to various fungicides including mancozeb, metalaxyl, chlorothalonil, fosetyl aluminium, potassium phosphonate and dimethomorph, of which metalaxyl was the most potent inhibitor of colony growth. Our findings provide updated information on the current Phytophthora species associated with gummosis in citrus in Northern Vietnam, with P. mekongensis being recorded for the first time in this region. This will serve as a reference for Phytophthora studies on citrus in the future and for developing an integrated management strategy for Phytophthora disease.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Current status and characterization of Phytophthora species associated with gummosis of citrus in Northern Vietnam

Tran

Vvedensky

et al. 2023

Journal of Phytopathology

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Isolation and Identification of Postharvest Rot Pathogens in Citrus × tangelo and Their Potential Inhibition with Acidic Electrolyzed Water

Ji,

Wang,

Liu

et al. 2024

Food Environ Virol

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This study focused on the identification of rot-causing fungi in Citrus × tangelo (tangelo) with a particular emphasis on investigating the inhibitory effects of acidic electrolyzed water on the identified pathogens. The dominant strains responsible for postharvest decay were isolated from infected tangelo fruits and characterized through morphological observation, molecular identification, and pathogenicity detection. Two strains were isolated from postharvest diseased tangelo fruits, cultured and morphologically characterized, and had their gene fragments amplified using primers ITS1 and ITS4. The results revealed the rDNA-ITS sequence of two dominant pathogens were 100% homologous with those of Penicillium citrinum and Aspergillus sydowii. These isolated fungi were confirmed to induce tangelo disease, and subsequent re-isolation validated their consistency with the inoculum. Antifungal tests demonstrated that acidic electrolyzed water (AEW) exhibited a potent inhibitory effect on P. citrinum and A. sydowii, with EC50 values of 85.4 μg/mL and 60.12 μg/mL, respectively. The inhibition zones of 150 μg/mL AEW to 2 kinds of pathogenic fungi were over 75 mm in diameter. Furthermore, treatment with AEW resulted in morphological changes such as bending and shrinking of the fungal hyphae surface. In addition, extracellular pH, conductivity, and absorbance at 260 nm of the fungi hypha significantly increased post-treatment with AEW. Pathogenic morphology and IST sequencing analysis confirmed P. citrinum and A. sydowii as the primary pathogenic fungi, with their growth effectively inhibited by AEW.

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Exploring eco-friendly solutions for Phytophthora disease management: Harnessing the anti-oomycete potential of a fermented lemon waste formulation

La Spada,

Bua,

Pane

et al. 2024

Journal of Agriculture and Food Research

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Pathogenicity and fungicide sensitivity of Phytophthora parvispora, a new pathogen causing gummosis and root rot disease on citrus trees

Cited by 7 publications

References 19 publications

Current status and characterization of Phytophthora species associated with gummosis of citrus in Northern Vietnam

Current status and characterization of Phytophthora species associated with gummosis of citrus in Northern Vietnam

Isolation and Identification of Postharvest Rot Pathogens in Citrus × tangelo and Their Potential Inhibition with Acidic Electrolyzed Water

Exploring eco-friendly solutions for Phytophthora disease management: Harnessing the anti-oomycete potential of a fermented lemon waste formulation

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