Pathogenicity and inactivated vaccine treatment of Aeromonas veronii JW-4 on crucian carp

Guo, Kefan; Sun, Yu; Tang, Xiaoqi; Zhou, Xicheng; Jiang, Mi; Yang, Qinglin; Li, Yanhong; Wu, Zhengli

doi:10.1016/j.micpath.2023.106315

Cited by 5 publications

(4 citation statements)

References 51 publications

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“…In our study, plasma GPT and GOT activities were significantly increased to the highest levels at 24 h after A. hydrophila infection and decreased gradually at the later infective stages, indicating that A. hydrophila infection led to severe hepatic damage at the initial stage of infection. Similarly, GPT and GOT activities were elevated in Japanese flounder in response to heat stress (20) and in crucian carp after A. veronii challenge (21).…”

Section: Plasma Biochemical Indices Changed After Infectionmentioning

confidence: 87%

“…Previous studies have found that aquatic animal AKP activities can be improved in response to either nutritive or environmental stress (42). Plasma AKP activity in the Chinese mitten crab was enhanced after A. hydrophila infection (21). Furthermore, A. veronii challenge increased plasma AKP activity in loaches (43).…”

Section: Plasma Biochemical Indices Changed After Infectionmentioning

confidence: 94%

“…These two enzymes, generally existing in hepatocytes, are secreted into the blood when the permeability of the hepatic cell membrane becomes damaged (20). Alkaline phosphatase (AKP), existing in macrophage lysosomes, has important functions in immunity, as it can mitigate the negative effects of lipopolysaccharides and alleviate inflammation (21). One of the functions of catalase (CAT), as an endogenous antioxidant enzyme, is to remove excessive reactive oxygen species (ROS) generated by bacterial infection (22).…”

Section: Introductionmentioning

confidence: 99%

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Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Ji,

Chen,

Zhu

et al. 2024

Front. Immunol.

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IntroductionAeromonas hydrophila, a bacterium widely distributed in the natural environment, causes multiple diseases in various animals. Exploring the mechanism of the host defense against A. hydrophila can help develop efficient strategies against Aeromonas infection.MethodsHerein, we investigated the temporal influence of A. hydrophila on the Chinese soft-shelled turtle, an economically important species, at the biochemical, transcriptomic, and metabolomic levels. Plasma parameters were detected with the test kits. Transcriptome and metabolome were respectively applied to screen the differentially expressed genes and metabolites.ResultsThe contents or activities of these plasma parameters were significantly increased at 24 hpi and declined at 96 hpi, indicating that 24 and 96 hpi were two important time points during infection. Totals of 3121 and 274 differentially expressed genes (DEGs) from the transcriptome while 74 and 91 differentially abundant metabolites (DAMs) from the metabolome were detected at 24 and 96 hpi. The top DEGs at 24 hpi included Ccl2, Ccl3, Ccl4, Il1β, Il6, Il7, Il15, Tnf, and Tnfr1 while Zap70, Cd3g, Cd8a, Itk, Pik3r3, Cd247, Malt1, and Cd4 were the most abundant at 96 hpi. The predominant DAMs included O-phospho-L-serine, γ-Aminobutyric acid, orotate, L-tyrosine, and L-tryptophan at 24 hpi, as well as L-glutamic acid, L-arginine, glutathione, glutathione disulfide, and citric acid at 96 hpi.DiscussionThe combined analysis of DEGs and DAMs revealed that tryptophan metabolism, nicotinate and nicotinamide metabolism, as well as starch and sucrose metabolism, were the most important signaling pathways at the early infective stage while tyrosine metabolism, pyrimidine metabolism, as well as alanine, aspartate and glutamate metabolism were the most crucial pathways at the later stage. In general, our results indicated that the Chinese soft-shelled turtle displays stage-specific physiological responses to resist A. hydrophila infection.

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Section: Plasma Biochemical Indices Changed After Infectionmentioning

confidence: 87%

Section: Plasma Biochemical Indices Changed After Infectionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Ji,

Chen,

Zhu

et al. 2024

Front. Immunol.

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show abstract

“…Aeromonas is widely distributed in freshwater and sewage environments and can also be isolated from food, such as meat, aquatic products, and milk, posing certain hazards to humans and aquatic animals (4)(5)(6). Aeromonas veronii (A.veronii), one of the main pathogens in the Aeromonas genus, can infect many species of freshwater and marine fish, including Carassius auratus, Tilapia, Catfish, Largemouth bass, Loach, Salmon, and Turbot, as well as Crustaceans and Mollusks (7)(8)(9). Clinical symptoms of infection mainly include septicemia, gill rot, enteritis, skin ulcers, and visceral infections.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the efficacy of Aeromonas veronii ΔhisJ vaccine in Carassius auratus via different immunization routes

Wu,

Ma,

Pan

et al. 2024

Front. Vet. Sci.

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IntrodctionAeromonas veronii is a significant pathogen to various aquatic life. Infections in fish can lead to high mortality rates, causing substantial economic losses in aquaculture. Vaccination is proposed as a substitute for antibiotics in aquaculture to decrease disease-related mortality and morbidity. Our study previously constructed a hisJ-deleted strain of A. veronii, which provided protective effect to Loach.MethodsTo further assess the vaccine’s applicability, this study evaluated its genetic stability and safety, and the immune protective effects in Carassius auratus through four distinct administration routes: intraperitoneal injection, intramuscular injection, oral administration, and immersion, to determine the efficacy of these administration routes.ResultsThe results showed that the vaccine remained genetically stable after 45 generations. Immunization via these administration routes was safe for Carassius auratus, with intraperitoneal and intramuscular injections causing stronger adverse reactions. Immersion immunization resulted in mild adverse reactions, and no significant adverse reactions were observed following oral immunization. Immunizing Carassius auratus at safe concentrations via these routes enhanced the phagocytic activity in serum, increased the levels of non-specific immune-related enzymes (ACP, AKP, C3, C4, LZM, SOD, and IgM), and improved specific serum antibody levels. It also elevated levels of cytokines related to inflammatory responses (IL-1β, IL-10, TNF-α, TGF-β) in organ tissues (liver, spleen, kidney, mid-post intestine, and gills). The survival rates of Carassius auratus were measured after challenging with the virulent strain A. veronii TH0426, resulting in the relative survival rates of 64% for Intraperitoneal vaccine group, 56% for Intramuscular vaccine group, 52% for oral vaccine group, and 48% for immersion vaccine group. Analysis of bacterial load in the liver, spleen, and kidney post-challenge showed a decreasing trend in the control group, indicating that the vaccine strain ΔhisJ could gradually restrict the rapid proliferation of bacteria in these tissues, thereby providing a certain level of immune protection against A. veronii.DiscussionIn brief, the vaccine strain ΔhisJ can serve as a safe live attenuated vaccine for Carassius auratus, and this study lays the foundation for the development of live attenuated vaccines against Aeromonas veronii.

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The role of aroA and ppk1 in Aeromonas veronii pathogenicity and the efficacy evaluation of mutant strain AV-ΔaroA/ppk1 as a live attenuated vaccine

Lu,

Ding,

Wei

et al. 2024

Fish & Shellfish Immunology

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Pathogenicity and inactivated vaccine treatment of Aeromonas veronii JW-4 on crucian carp

Cited by 5 publications

References 51 publications

Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Comparison of the efficacy of Aeromonas veronii ΔhisJ vaccine in Carassius auratus via different immunization routes

The role of aroA and ppk1 in Aeromonas veronii pathogenicity and the efficacy evaluation of mutant strain AV-ΔaroA/ppk1 as a live attenuated vaccine

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