Pathogenicity and real-time PCR detection of<i>Fusarium</i>spp. in wheat and barley roots

Strausbaugh, C.A.; Overturf, Ken; Koehn, Anita C.

doi:10.1080/07060660509507242

Cited by 49 publications

(31 citation statements)

References 24 publications

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“…The fact that F. semitectum, F. reticulatum, F. equiseti, and F. acuminatum were the dominant species isolated from the drier Power County location is also consistent with previous observations from this county (Strausbaugh et al 2004). Some of the same species are known to be associated with cereal roots in the PNW (Smiley and Patterson 1996) and have been shown to be associated with root and crown infections (Fedel-Moen and Harris 1987;Strausbaugh et al 2004Strausbaugh et al , 2005. The importance of the role these Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

“…cultures were placed under fluorescent light for 3 weeks and identified as described by Nelson et al (1983). Root isolation data from 2003 had been utilized previously to support the publication of a real-time polymerase chain reaction assay (Strausbaugh et al 2005).…”

Section: Root Evaluationsmentioning

confidence: 99%

“…Shoemaker are the primary root rot pathogens of concern at higher elevations in the IMW region (Strausbaugh et al 2004(Strausbaugh et al , 2005. Recently, among the Fusarium spp., F. acuminatum Ellis & Everh., F. reticulatum Mont., and F. semitectum Berk.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, among the Fusarium spp., F. acuminatum Ellis & Everh., F. reticulatum Mont., and F. semitectum Berk. & Ravenel, normally considered minor pathogens, have been implicated as part of a Fusarium complex affecting crops in this production system (Strausbaugh et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Influence of subsoiling on direct-seeded cereals in southeastern Idaho

Strausbaugh

Windes

2006

Canadian Journal of Plant Pathology

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Abstract:The influence of shallow (20 cm deep) subsoil tillage on cereals planted with a no-till planter was investigated for 3 years at two locations in southeastern Idaho. Among Fusarium spp., F. culmorum was the primary fungus isolated from diffuse brown-black root lesions in the wetter location (Ririe), while F. semitectum, F. reticulatum, F. equiseti, and F. acuminatum were the dominant species isolated at the drier location (Arbon Valley). Increased yields resulting from methyl bromide fumigation indicated that biological factors were limiting the yield. However, subsoiling did not influence nematode populations or fungal root rots. The primary parasitic nematode found at both locations was Pratylenchus neglectus. Subsoiling had a tendency to increase soil moisture at depths between 61 and 90 cm, to increase organic matter, and to decrease nitrogen. Yield increased by 8% or more with subsoil tillage at both locations the first year. Yield increases were not significant in other years, when moisture was particularly limiting or abundant. Subsoiling may prove favorable for cereals planted in a soil-conservation tillage system, but the risk of fungal root rots may increase with such a practice. Additional study is warranted, as subsoil-tillage practices may increase yield and desirable soil parameters without compromising the benefits associated with cereal production in a soil-conservation tillage system. Key words: Fusarium spp., fusarium root rot, direct seeding, dryland, subsoiling, no till, zero tillage.Résumé : L'effet d'un sous-solage superficiel (20 cm de profondeur) sur des céréales plantées avec un planteur pour le semis direct a été étudié pendant 3 ans à deux sites du sud-est de l'Idaho. Parmi les espèces du Fusarium, le F. culmorum a été le principal champignon isolé de lésions racinaires floues brun noir au site le plus humide (Ririe), alors que les F. semitectum, F. reticulatum, F. equiseti et F. acuminatum ont été les espèces dominantes isolées du site le plus sec (vallée d'Arbon). Des augmentations de rendement résultant de fumigation au bromure de méthyle ont révélé que des facteurs biologiques limitaient les rendements. Par contre, le sous-solage n'a pas eu d'effet sur les populations de nématodes ou sur les pourritures racinaires fongiques. Le Pratylenchus neglectus fut le principal nématode parasite retrouvé aux deux sites. Le sous-solage avait tendance à augmenter la teneur en eau du sol aux profondeurs entre 61 et 90 cm, à augmenter la matière organique et à diminuer l'azote. Avec le sous-solage, les rendements ont augmenté de 8% ou plus aux deux sites au cours de la première année. Lors des autres années, les augmentations de rendement n'étaient pas significatives lorsque l'eau était particulièrement limitée ou abondante. Le sous-solage peut s'avérer profitable pour les céréales plantées dans un système de conservation des sols, mais une telle pratique pourrait accroître le risque de pourriture racinaire fongique. Des études supplémentaires sont souhaitables puisque les pratiques de so...

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Section: Discussionmentioning

confidence: 99%

Section: Root Evaluationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of subsoiling on direct-seeded cereals in southeastern Idaho

Strausbaugh

Windes

2006

Canadian Journal of Plant Pathology

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“…In the majority of these cases, PCR was only used for specific detection of the pathogen in plant material, but not for its quantification. To date there have been several reports of the use of real-time quantitative PCR for quantification of different pathogens (BATES et al 2001;McNEIL et al 2004;STRAUSBAUGH et al 2005). This paper was aimed at the development of specific primers for detection and preliminary study of quantification of R. secalis in seed samples of barley.…”

mentioning

confidence: 99%

Rapid Detection and Quantification of Rhynchosporium secalis in Barley Using a Polymerase Chain Reaction

Gubiš¹,

Hudcovicová²,

Gubišová³

2006

CZECH J GENET PLANT

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PCR primers for diagnosis of Rhynchosporium secalis in seed samples of barley were developed. For the quantification of the pathogen in seed samples a real-time PCR with SYBR Green approach was used. Amounts from 1.8 to 419.1 pg of R. secalis DNA per 100 ng of total DNA were detected in 18 samples of barley seeds contaminated by R. secalis in field conditions. The correctness of this quantitative analysis was checked using an artificial infection of seeds with 1, 2, 5 and 20% level of infection by R. secalis. The level of contamination of artificially infected samples decreased with a lowering amount of added seed powder contaminated by the pathogen, the correlation coefficient for this analysis was 0.98. While the primer pair used in these analyses shows cross-reactions with other pathogens (P. teres, Drechslera tritici-repentis, F. culmorum and F. poe), it is recommended to check the products of RT-PCR by agarose-gel electrophoresis, in which these pathogens are easily distinguishable from R. secalis by different lengths of the amplified fragments.

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