Pathogenicity and toxigenicity of Fusarium verticillioides isolates collected from maize roots, stems and ears in South Africa

Schoeman, A. S.; Flett, B. C.; Rensburg, B. Janse van; Ncube, Edson; Viljoen, Altus

doi:10.1007/s10658-018-1510-z

Cited by 11 publications

(12 citation statements)

References 59 publications

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“…Fusarium moniliforme (synonym = F. verticillioides ), Aspergillus niger, and Botrytis cinereaall exhibit widespread impact on food crops. F. moniliforme (Pamphile & Azevedo, 2002) can produce mycotoxins with toxic secondary metabolites to both humans and animals (Schoeman et al, 2018). It is one of the most significant fungal pathogens to infect corn (Munkvold, 2003).…”

Section: Resultsmentioning

confidence: 99%

Engineering of Polygalacturonase-Inhibiting Protein as an Ecological, Friendly, and Non-toxic Pest Control Agent

Chiu

Behari

Chartron

et al. 2021

Preprint

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Fungal pathogens cause extensive plant diseases that damage crop production in the agricultural industry, resulting in annual crop loss, diminished food security, and historically significant epidemics. Though effective fungicides are available, their risks to the environment and animal health have increased the demand for more sustainable methods to control fungal pathogens. In plants, polygalacturonic-inhibiting proteins (PGIPs) play critical roles for resistance to fungal disease by inhibiting the pectin-depolymerizing activity of endopolygalacturonases (PGs), one type of enzyme secreted by pathogens that compromise plant cell walls and leave the plant susceptible to disease. Here, the interactions between PGIPs from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) and PGs from Aspergillus niger (AnPG2), Botrytis cinerea (BcPG1, BcPG2), and Fusarium moniliforme (FmPG3) were reconstituted through a yeast two hybrid (Y2H) system to investigate the inhibition efficiency of various PvPGIP1 and 2 truncations and mutants. We found that tPvPGIP2_5-8, which contains LRR5 to LRR8 and is of only one-third the size of the full-length peptide, exhibits the same level of interactions with AnPG and BcPGs as the full length PvPGIP2 via Y2H. The inhibitory activities of tPvPGIP2_5-8 on the growth of A. niger were then examined and confirmed on pectin agar. Application of both full length PvPGIP2 and tPvPGIP2_5-8 clearly slows down the growth of A. niger and B. cinerea in the presence of pectin. The investigation on the sequence-function correlation of PvPGIP2 suggests that LRR5 could have the most essential structural feature for the inhibitory activities, and may be a possible target for the future engineering of PGIP with enhanced activity. This work highlights the potential of using plant-derived PGIPs as an exogenously applied fungal control agent both to plants and postharvest crops while minimally impacting the environment and human health.

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Section: Resultsmentioning

confidence: 99%

Engineering of Polygalacturonase-Inhibiting Protein as an Ecological, Friendly, and Non-toxic Pest Control Agent

Chiu

Behari

Chartron

et al. 2021

Preprint

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“…Fusarium verticillioides was isolated from a single farm where the Bambara plants were interspersed with maize ( Zea mays L). Fusarium verticillioides has been reported as being the most prevalent fungus in maize growing regions in South Africa (Schoeman, Flett, Janse van Rensburg, Ncube, & Viljoen, 2018). It was therefore possibly transferred to the soil and subsequently to the BGN pods through cross contamination.…”

Section: Resultsmentioning

confidence: 99%

Contamination of freshly harvested Bambara groundnut (Vigna subterranea) seed from Mpumalanga, South Africa, with mycotoxigenic fungi

Otto

Pretorius

Kritzinger

et al. 2020

Journal of Food Safety

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Freshly harvested Bambara groundnut (BGN) is occasionally consumed raw and can potentially become infected with mycotoxingenic field fungi. In this study, BGN samples were obtained from 12 farms in three districts of Mpumalanga in South Africa. Eight pooled samples were screened for multi-mycotoxin contamination using Ultra Performance Liquid-Chromatography tandem mass spectrometry (UPLC-MS/MS). To identify mycoflora, 12 samples were screened using conventional and molecular methods. Selected potential mycotoxin producing isolates were screened for mycotoxins using UPLC-MS/MS. No mycotoxins were detected on the freshly harvested BGN samples, but they were infected with various mycotoxin producing fungal species namely Aspergillus flavus (50%), Penicillium citrinum (25%), Penicillium oxalicum (17%), Penicillium citreoviridin (0.8%), and Fusarium verticillioides (0.8%). Following screening of selected fungal cultures, aflatoxin B 1 (0.4, 0.45 and 0.4 ppm) and fumonisin B 1 (0.7 ppm) were detected from A. flavus and F. verticillioides, respectively. Identification of mycotoxigenic fungi on freshly harvested BGN presents a potential health risk.

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“…Fusarium ear rot (FER) of maize is caused by Fusarium verticillioides (Saccardo) Nirenberg, Fusarium proliferatum (Matsushima) Nirenberg, Fusarium subglutinans (Saccardo) Nirenberg and Fusarium temperatum (Scauflaire and Munaut) (Schoeman et al, 2018). These are all mycotoxigenic pathogens that affect maize (Zea mays L.) at all stages of development (Foley, 1962;Munkvold, 2003a).…”

Section: Introductionmentioning

confidence: 99%

“…These are all mycotoxigenic pathogens that affect maize (Zea mays L.) at all stages of development (Foley, 1962;Munkvold, 2003a). Of the FER pathogens, F. verticillioides is considered to be the most important in Southern Africa (Boutigny et al, 2012;Schoeman et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The association of maize characteristics with resistance toFusarium verticillioides and fumonisin accumulation in commercial maize cultivars

Links

Zyl

Cassiem

et al. 2020

WMJ

Self Cite

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Fusarium verticillioides is the primary fungus that causes Fusarium ear rot (FER) of maize. Infection results in reduced grain yield and quality due to moulding and the contamination of grain with toxic compounds namely fumonisins. Resistance to fungal infection and fumonisin accumulation in maize and maize grain is governed at different levels. In this study, the structural, physico-chemical and genetic basis of resistance to F. verticillioides was investigated in two, replicated field trials at Potchefstroom and Vaalharts in South Africa. Phenotypic data (silk length, husk coverage, pericarp thickness hundred-kernel mass and kernel hardness), physico-chemical data (kernel pH, moisture content, total nitrogen and carbon as well as phenolic acid content) and the expression of pathogenesis-related-5 gene (PR5) and peroxidase gene expression was evaluated in 15 commercial cultivars under artificially inoculated and natural infection conditions. The data were correlated to FER severity, fumonisin accumulation and fungal DNA (referred to as infection indicators). Disease development and fumonisin contamination in Vaalharts was significantly more than in Potchefstroom. There were no significant correlations (r=≥0.60) between phenotypic characteristics and infection indicators. Kernel pH was the most important trait associated with disease development and was negatively correlated (between r=-0.58 and r=-0.75) to all infection indicators. PR5 gene expression had significant positive correlations (r=0.69 and r=0.72) with the fungal and fumonisin levels, respectively. This study presents of the first data demonstrating the use of gene expression in identifying FER/fumonisin-resistant plant material and could aid breeders and growers in selecting resistant material more effectively.

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Pathogenicity and toxigenicity of Fusarium verticillioides isolates collected from maize roots, stems and ears in South Africa

Cited by 11 publications

References 59 publications

Engineering of Polygalacturonase-Inhibiting Protein as an Ecological, Friendly, and Non-toxic Pest Control Agent

Engineering of Polygalacturonase-Inhibiting Protein as an Ecological, Friendly, and Non-toxic Pest Control Agent

Contamination of freshly harvested Bambara groundnut (Vigna subterranea) seed from Mpumalanga, South Africa, with mycotoxigenic fungi

The association of maize characteristics with resistance toFusarium verticillioides and fumonisin accumulation in commercial maize cultivars

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