Pathogenicity and virulence of black-pigmented Gram-negative anaerobes

Sundqvist, Göran

doi:10.1111/j.1574-695x.1993.tb00315.x

Cited by 60 publications

(28 citation statements)

References 104 publications

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“…The mean concentration of endotoxin in samples from patients with spontaneous pain was 3.7 mg/mL, whereas it was 2.4 mg/mL in asymptomatic cases, indicating a positive correlation between the concentration of endotoxin in the root canal and the presence of endodontic signs and symptoms (25). In the present study, we used LPS from P. gingivalis, one of the most pathogenic species in the group of black-pigmented gram-negative anaerobes (26). To induce pulpal cell inflammation in vitro, cells were treated with 1 mg/mL P. gingivalis LPS (27) and 1 mg/mL Prevotella intermedia LPS (28).…”

Section: Discussionmentioning

confidence: 93%

Role of SIRT1 in Heat Stress- and Lipopolysaccharide-induced Immune and Defense Gene Expression in Human Dental Pulp Cells

Lee

Min

Bae

et al. 2011

Journal of Endodontics

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Section: Discussionmentioning

confidence: 93%

Role of SIRT1 in Heat Stress- and Lipopolysaccharide-induced Immune and Defense Gene Expression in Human Dental Pulp Cells

Lee

Min

Bae

et al. 2011

Journal of Endodontics

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“…In the present study, we used LPS from P. gingivalis , one of the most pathogenic species in the group of black-pigmented Gram-negative anaerobes (22). Other investigators have found P. endodontalis in 53% and P. gingivalis in 12% of periapical abscesses with endodontic origin (4).…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide From Porphyromonas gingivalis Sensitizes Capsaicin-Sensitive Nociceptors

Ferraz

Henry

Hargreaves

et al. 2011

Journal of Endodontics

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Although odontogenic infections are often accompanied by pain, little is known about the potential mechanisms mediating this effect. In this study, we tested the hypothesis that trigeminal nociceptive neurons are directly sensitized by lipopolysaccharide (LPS) isolated from an endodontic pathogen, Porphyromonas gingivalis (P. gingivalis). In vitro studies conducted with cultures of rat trigeminal neurons demonstrated that pretreatment with LPS produced a significant increase in the capsaicin-evoked release of calcitonin gene-related peptide (CGRP) when compared to vehicle pretreatment, thus showing sensitization of the capsaicin receptor, TRPV1, by LPS. Furthermore, confocal microscopic examination of human tooth pulp samples showed the colocalization of the LPS receptor (toll-like receptor 4; TLR4) with CGRP containing nerve fibers. Collectively, these results suggest the direct sensitization of nociceptors by LPS at concentrations found in infected canal systems as one mechanism responsible for the pain associated with bacterial infections.

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“…The importance of Porphyromonas gingivalis as a causative agent in adult peridontitis is well documented ( 13, 16, 31). This organism, a black‐pigmented, gram‐negative anaerobic rod, possesses several putative virulence factors that can directly affect the periodontium or elicit host functions that result in destruction typical of advanced periodontitis.…”

mentioning

confidence: 99%

Unaltered expression of the major protease genes in a non‐virulent recA‐defective mutant of Porphyromonas gingivalis W83

Abaibou¹,

Ma²,

Olango³

et al. 2000

Oral Microbiology and Immunology

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Porphyromonas gingivalis FLL32, a recA mutant, was isolated during construction of a recA defective mutant of P. gingivalis W83 by allelic exchange mutagenesis. In contrast to W83 and FLL33, the typical recA- mutant previously reported, FLL32 was non-pigmented, lacked beta-hemolytic activity on blood agar and produced significantly less proteolytic activity. The proteolytic activity in FLL32 was mostly soluble. Expression of the rgpA, rgpB and kgp protease genes was unaltered in FLL32 when compared to FLL33 and the wild-type strain. FLL32 exhibited reduced virulence in a murine model and partially protected the animals immunized with that strain against a subsequent lethal challenge by the wild-type strain. These results indicate that the reduced level of proteolytic activity in FLL32 may be due to a defect in the processing of the proteases. Further, immunization with a non-virulent recA defective mutant of P. gingivalis can partially protect against a lethal wild-type challenge. The results from this study suggest that the recA locus may be involved in expression and regulation of proteolytic activity in P. gingivalis.

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Pathogenicity and virulence of black-pigmented Gram-negative anaerobes

Cited by 60 publications

References 104 publications

Role of SIRT1 in Heat Stress- and Lipopolysaccharide-induced Immune and Defense Gene Expression in Human Dental Pulp Cells

Role of SIRT1 in Heat Stress- and Lipopolysaccharide-induced Immune and Defense Gene Expression in Human Dental Pulp Cells

Lipopolysaccharide From Porphyromonas gingivalis Sensitizes Capsaicin-Sensitive Nociceptors

Unaltered expression of the major protease genes in a non‐virulent recA‐defective mutant of Porphyromonas gingivalis W83

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