Pathogenicity of Aeromonas hydrophila in High-value Native Pangasius Catfish, Pangasius nasutus (Bleeker)

Abstract

Pangasius catfish, Pangasius nasutus, is a promising candidate for aquaculture due to its high market value. However, the presence of pathogenic bacteria in Aeromonas hydrophila is a major concern in P. nasutus farming in this country. This study determines the pathogenicity of A. hydrophila in P. nasutus. A total of 80 P. nasutus juveniles were intraperitoneally injected with 0, 103, 105, and 107 CFU mL-1 of A. hydrophila and monitored until 240 hr. The infected moribund fish’s kidneys, livers, and spleens were collected for histopathological analysis. The LD50-240hr value was found at 0.8 × 104 CFU/ml of A. hydrophila. The percentage of mortality in 0, 103, 105, and 107 CFU/ml infected groups were found to be at 0, 40, 60, and 90%, respectively. The infected fish showed congestion at the base of the fin, ascites, enlarged gall bladder, and swollen spleen. It is the earliest report on A. hydrophila’s pathogenicity in high-value native fish, P. nasutus.

Molecular characterization of Aeromonas hydrophila detected in Channa marulius and Sperata sarwari sampled from rivers of Punjab in Pakistan

“…Likewise, Li, [77] found 100% similarity in the phylogenetic relationships of the 16S rRNA gene of A. hydrophila in China. In a recent study, Sani,[93] also observed 100% similarity in the phylogenetic relationships of the 16S rRNA gene of A. hydrophila in Malaysia, which corroborated our results.…”