Pathogenicity of clinical Salmonella enterica serovar Typhimurium isolates from Thailand in a mouse colitis model

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium [STM]) is a leading cause of nontyphoidal salmonellosis (NTS) worldwide. The pathogenesis of NTS has been studied extensively using a streptomycinpretreated mouse colitis model with the limited numbers of laboratory STM strains. However, the pathogenicity of the clinically isolated STM (STMC) strains endemic in Thailand in mice has not been explored. The aim of this study was to compare the pathogenicity of STMC strains collected from Northern Thailand with the laboratory STM (IR715) in mice. Five STMC isolates were obtained from the stool cultures of patients with acute NTS admitted to Maharaj Nakorn Chiang Mai Hospital in 2016 and 2017. Detection of virulence genes and sequence type (ST) of the strains was performed. Female C57BL/6 mice were pretreated with streptomycin sulfate 1 day prior to oral infection with STM. On Day 4 postinfection, mice were euthanized, and tissues were collected to analyze the bacterial numbers, tissue inflammation, and cecal histopathological score. We found that all five STMC strains are ST34 and conferred the same or

Anti-inflammatory Effect of Probiotic Limosilactobacillus reuteri KUB-AC5 Against Salmonella Infection in a Mouse Colitis Model

“…Mice in the untreated control group were daily fed with sterile PBS. To induce colitis, 100 μL of (200 mg/mL) streptomycin sulfate (Sigma Aldrich, Singapore) solution was given orally to mice 1 day prior to STM infection (10 9 cfu of STM/mouse) ( Barthel et al, 2003 ; Sarichai et al, 2020 ). All mice were euthanized at day 4 post infection.…”

“…Four days after the infection with STM, all mice were euthanized and tissue samples were collected for mRNA expressions by qPCR. Fold change (relative of expression) of important proinflammatory cytokine genes [ Kc , Ifng , and Il-6 encoding for KC or CXCL-1, interferon (IFN)-gamma and IL-6, respectively], Nos2 and Zo-1 [encoding for iNOS and a tight junction protein zonula occludens (ZO-1), respectively], were performed as previously described ( Sarichai et al, 2020 ). In short, collected tissues were immersed in RNA preservative solution (RNAstore reagent, TIANGEN, China) and stored at −20°C until extraction.…”

“…Analysis was carried out using a parametric test [one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test]. The cecal histopathological scores were statistically tested using a Kruskal–Wallis test with multiple comparisons as previously described ( Sarichai et al, 2020 ). P < 0.05 was considered statistically significant; ∗ , ∗∗ , and ∗∗∗ indicate P -values < 0.05, 0.01, and 0.001, respectively.…”

Physiological Characteristics of Bacterial Droplets Indicate a Catastrophic Consequence With an Increase in Impact Velocity

“…Salmonella is a common bacterial type that severely impacts the feed supply chain and various food production industries 7 . It has been reported that thousands of people die worldwide from Salmonella systemic infections like non-typhoidal salmonellosis (NTS), brain culminating in meningitis, and associated neurological abnormalities 8–12 . Moreover, Salmonella continues to be the second biggest cause of foodborne disease in the United States and the leading cause of both hospitalization and deaths 7 .…”

Effect of Inonotus obliquus polysaccharide on composition of the intestinal flora in mice with acute endometritis

“…The mice were randomly divided into four groups with twelve mice in: Control group; LPS group; LPS+IOP group. To avoid infection by other bacteria, mice were pretreated with streptomycin [27]. A murine model of LPS-induced endometritis was established as previously reported [28].…”