Pathogenicity of different H5N6 highly pathogenic avian influenza virus strains and host immune responses in chickens

Chen, Zuxian; Wang, Zhenyu; Zhao, Xiya; Guan, Yun; Xue, Qian; Li, Jinrong; Liu, Zhiting; Zhao, Bingbing; He, Zhuoliang; Huang, Jianni; Liao, Ming; Song, Yafen; Jiao, Peirong

doi:10.1016/j.vetmic.2020.108745

Cited by 2 publications

(4 citation statements)

References 42 publications

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“…Frontiers in Microbiology 15 frontiersin.org IFN-I, IL-6, OAS, IL-8, and Mx1 in infected chickens, causing immune damage and an excessive inflammatory response, which accelerates the death of infected chickens (Wei et al, 2013;Song et al, 2019;Chen et al, 2020). In this study, two H5N6 HPAIVs (Q135 and S23) replicated in all tested tissues of chickens.…”

Section: Discussionmentioning

confidence: 56%

“…A low dose (10 3 EID 50 /0.1 mL) of the H5N1 virus GGD1-96 strain did not cause death in inoculated chickens; however, the same dose of H5N1 HPAIV isolated in 2012 caused illness and death in inoculated chickens ( Yuan et al, 2014 ). The H5 subtype HPAIV induces the overexpression of cytokines such as IFN-I, IL-6, OAS, IL-8, and Mx1 in infected chickens, causing immune damage and an excessive inflammatory response, which accelerates the death of infected chickens ( Wei et al, 2013 ; Song et al, 2019 ; Chen et al, 2020 ). In this study, two H5N6 HPAIVs (Q135 and S23) replicated in all tested tissues of chickens.…”

Section: Discussionmentioning

confidence: 99%

“…However, some H5N6 AIVs do not kill chickens but spread silently through flocks ( Mei et al, 2019 ; Uchida et al, 2019 ). The H5N6 HPAIVs that have become prevalent in recent years are mainly transmitted in bird populations through direct contact with droppings or through the oral and nasal secretions of infected birds ( Qu et al, 2019 ; Chen et al, 2020 ). The H5N6 virus can also be transmitted via air and aerosols in crowded flocks, such as in crowded live-bird markets ( Zhou et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens

Wang

Yang

et al. 2023

Front. Microbiol.

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Waterfowl, such as ducks, are natural hosts for avian influenza viruses (AIVs) and act as a bridge for transmitting the virus to humans or susceptible chickens. Since 2013, chickens and ducks have been threatened by waterfowl-origin H5N6 subtype AIVs in China. Therefore, it is necessary to investigate the genetic evolution, transmission, and pathogenicity of these viruses. In this study, we determined the genetic characteristics, transmission, and pathogenicity of waterfowl-origin H5N6 viruses in southern China. The hemagglutinin (HA) genes of H5N6 viruses were classified into the MIX-like branch of clade 2.3.4.4h. The neuraminidase (NA) genes belonged to the Eurasian lineage. The PB1 genes were classified into MIX-like and VN 2014-like branches. The remaining five genes were clustered into the MIX-like branch. Therefore, these viruses belonged to different genotypes. The cleavage site of the HA proteins of these viruses was RERRRKR/G, a molecular characteristic of the H5 highly pathogenic AIV. The NA stalk of all H5N6 viruses contained 11 amino acid deletions at residues 58–68. All viruses contained 627E and 701D in the PB2 proteins, which were molecular characteristics of typical bird AIVs. Furthermore, this study showed that Q135 and S23 viruses could replicate systematically in chickens and ducks. They did not cause death in ducks but induced mild clinical signs in them. All the infected chickens showed severe clinical signs and died. These viruses were shed from the digestive and respiratory tracts and transmitted horizontally in chickens and ducks. Our results provide valuable information for preventing H5N6 avian influenza outbreaks.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens

Wang

Yang

et al. 2023

Front. Microbiol.

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“…Thus, H5N6 genotypes diversified both in poultry and humans (11,(16)(17)(18). Genetic and biologic characteristics indicated that H5N6 viruses were highly pathogenic in chickens (19)(20)(21). Studies have shown that some H5N6 viruses of clade 2.3.4.4 possessed the ability to bind both avian-origin and human-origin sialic acid receptors and had the ability to attach to human tracheal epithelial and alveolar tissues (22).…”

Section: A(h5n6) Viruses Chinamentioning

confidence: 99%

Epidemiologic, Clinical, and Genetic Characteristics of Human Infections with Influenza A(H5N6) Viruses, China

Zhu¹,

Li²,

Dong³

et al. 2022

Emerg. Infect. Dis.

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Pathogenicity of different H5N6 highly pathogenic avian influenza virus strains and host immune responses in chickens

Cited by 2 publications

References 42 publications

Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens

Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens

Epidemiologic, Clinical, and Genetic Characteristics of Human Infections with Influenza A(H5N6) Viruses, China

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