Pathogenicity of indigenous Beauveria bassiana (Balsamo) against Ceratitis capitata Wiedemann (Diptera: Tephritidae) under laboratory conditions

Abstract

Background The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is the major insect pest of fruit production worldwide. Chemical control of this pest has induced the selection of resistant medflies populations and negative environmental impact. In addition, the use of pesticides has become an obstacle to the fresh agricultural products exported to many countries that restrict pesticides residues. The aim of this study was to propose alternatives based on the use of microbiological products for the control of C. capitata. Main body The pathogenicity of the Turkish isolate Beauveria bassiana BMAUM M6-4 was evaluated against immature stages and adult of C. capitata under laboratory conditions at a concentration of 107 conidia/ml via different routes of exposure. Conidial treatment was applied by spraying soil and filter paper against 3rd instar larvae and pupae. In contrast, the treatment was administered to adult males and females by contact and ingestion. The percentage of mortality was recorded on the 3rd, 7th, 12th and 15th day after inoculation. Obtained results showed that B. bassiana was very effective against the adult’s fly, where males were more susceptible than females in both treatments (contact and ingestion). This isolate was tested against 3rd instar larvae. It achieved an infection rate of 33.33 and 43.5% of the larvae treated on filter paper and in the soil, respectively. Application of conidial preparations to soil reduced insect emergence and CAN represent a promising strategy for fruit fly integrated management (57.33 and 56.66% emergence from the treatments in soil and filter paper). Conclusion Entomopathogenic fungi are promising as a biocontrol agent that can be used under different modes of ground application against larvae, prepupae and pupae, and/or as a bait spraying or contact against adults.

Diversity of fruit fly species in agricultural area Chott Zahrez Chergui (Djel-fa): First record of Trupanea amoena (Diptera: Tephritidae) in Algeria

“…Several fruit flies attack diverse species of fruit (El-Heneidy et al, 2019;Food and Agriculture Organization, 2015;Łabanowska & Piotrowski, 2015;Mahmoud et al, 2019;Nebie et al, 2021;Orhan et al, 2016). In Algeria, fruit fly pose a risk to most commercial fruit; some species of the Tephritidae family are introduced pests and are very common in Mitidja and in many regions where fruit production is intense (Chergui et al, 2020;Laamari et al, 2022;Mostefaoui et al, 2020;Settaoui et al, 2017). The dense vegetation cover of the steppes near the Chotts, particularly in Djelfa, provides a favourable environment for the development of arthropods harmful to agriculture and humans (Khadoumi, et al, 2022;Messaoudene et al, 2023).…”

Virulence of Five Isolates of Indigenous Beauveria Bassiana Against Eggs and Nymphs of Bemisia Tabaci Gennadius (Hemiptera: Aleyrodidae)

“…23,29,30,31 There was a difference in LT 50 between isolates (Table 3 death, and most conidia were recorded on legs, wings, and thoraces of some adult cadavers. 32 Cherguiet al 33 noted that dead individuals of Ceratitiscapitata were covered with a white mycelium characteristic of the fungus B. bassiana.…”

Microorganisms and Biological Pest Control: An Analysis Based on a Bibliometric Review

“…Microbial insecticide's effectiveness has been studied worldwide in a variety of pests of diverse crops, e.g., sugarcane [36], tobacco [37], wheat [38], potato [39], mango [40], tomato [41], and berries [42]. They have been evaluated under diverse conditions: laboratory [43], open fields [44] and greenhouses [45]. They had become a fundamental component for diverse integrated pest management programs [46] and organic production [47].…”