Pathogenicity of novel reassortant Eurasian avian-like H1N1 influenza virus in pigs

Sun, Haoran; Liu, Jiyu; Xiao, Yihong; Duan, Yuhong; Yang, Jinshui; Yu, Ying‐Hui; Li, Han; Zhao, Yuzhong; Pu, Juan; Sun, Yipeng; Sun, Honglei

doi:10.1016/j.virol.2021.06.001

Cited by 7 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Swine influenza viruses, as a zoonotic disease, has an important impact on public health [46]. G4 genotype reassortant Eurasian avian-like (EA) H1N1 virus possessing possessing 2009 pandemic (pdm/09) and triple-reassortant (TR)derived internal genes has replaced the G1 genotype EA H1N1 virus and has become predominant in swine populations in China [6,45,47]. To protect the human population and animals from precarious yearly epidemics and from the potential threat of new pandemics, only a broad-spectrum, efficient and non-adverse-effect vaccine should be qualified [48].…”

Section: Discussionmentioning

confidence: 99%

Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Zhang

et al. 2022

Microb Cell Fact

View full text Add to dashboard Cite

Background Swine influenza A virus (swIAV) is a major concern for the swine industry owing to its highly contagious nature and acute viral disease. Currently, most commercial swIAV vaccines are traditional inactivated virus vaccines. The Lactobacillus plantarum-based vaccine platform is a promising approach for mucosal vaccine development. Oral and intranasal immunisations have the potential to induce a mucosal immune response, which confers protective immunity. The aim of this study was to evaluate the probiotic potential and adhesion ability of three L. plantarum strains. Furthermore, a recombinant L. plantarum strain expressing the head domain of swIAV antigen HA1 was constructed and evaluated for its ability to prevent swIAV infection. Results The three L. plantarum strains isolated from healthy pig faecal samples maintained the highest survival rate when incubated at pH 3 and at bile salt concentration of 0.3%. They also showed high adherence to intestinal cells. All three L. plantarum strains were monitored in live mice, and no major differences in transit time were observed. Recombinant L. plantarum expressed swIAV HA1 protein (pSIP401-HA1-ZN-3) and conferred effective mucosal, cellular and systemic immune responses in the intestine as well as in the upper respiratory airways of mice. In conclusion, the oral and intranasal administration of L. plantarum strain pSIP401-HA1-ZN-3 in mice induced mucosal immunity and most importantly, provided protection against lethal influenza virus challenge. Conclusion In summary, these findings suggest that the engineered L. plantarum strain pSIP401-HA1-ZN-3 can be considered as an alternative approach for developing a novel vaccine during an swine influenza A pandemic.

show abstract

Section: Discussionmentioning

confidence: 99%

Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Zhang

et al. 2022

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…EA H1N1 were first isolated from swine in Hong Kong in 1993 [ 13 ]. Since 2001, EA H1N1 IAV-S has become the dominant circulating virus in China [ 9 , 14 ] and will preferentially bind to human-like receptors. Some of the viruses can be efficiently transmitted in ferrets through respiratory droplets [ 15 ], and preexisting immunity in humans may not be sufficient to overcome EAH1N1 infections [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Diverse influenza virus lineages are present in the pig population, including the classic swine H1N1 (CS H1N1) virus lineage, Eurasian avian-like H1N1 (EA H1N1) virus lineage, 2009 pandemic H1N1 (pdm/09 H1N1) virus lineage, North American triple-reassortant virus, and H3N2 virus lineage [ 9 , 10 ]. Despite collaborations on influenza research between international organizations such as the World Organization for Animal Health, Food and Agriculture Organization of the United Nations, and the World Health Organization being established to share information and resources [ 11 ], relatively little is known regarding the IAV-S circulating in swine in China and around the world.…”

Section: Introductionmentioning

confidence: 99%

Serological Surveillance of the H1N1 and H3N2 Swine Influenza A Virus in Chinese Swine between 2016 and 2021

Zhao

Han

Chen

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Background. Swine influenza A virus (IAV-S) is a common cause of respiratory disease in pigs and poses a major public health threat. However, little attention and funding have been given to such studies. The aim of this study was to assess the prevalence of the Eurasian avian-like H1N1 (EA H1N1), 2009 pandemic H1N1 (pdm/09 H1N1), and H3N2 subtype antibodies in unvaccinated swine populations through serological investigations. Such data are helpful in understanding the prevalence of the IAV-S. Methods. A total of 40,343 serum samples from 17 regions in China were examined using hemagglutination inhibition (HI) tests against EA H1N1, pdm/09 H1N1, and H3N2 IAV-S from 2016 to 2021. The results were analyzed based on a reginal distribution, seasonal distribution, and in different breeding stages. Results. A total of 19,682 serum samples out of the 40,343 were positive for IAV-S (48.79%). The positivity rates to the EA H1N1 subtype, pdm/09 H1N1 subtype, and H3N2 subtype were 24.75% (9,986/40,343), 7.94% (3,205/40,343), and 0.06% (24/40,343), respectively. The occurrences of coinfections from two or more subtypes were also detected. In general, the positivity rates of serum samples were related to the regional distribution and feeding stages. Conclusions. The results of this study showed that the anti-EA H1N1 subtype and pdm/09 H1N1 subtype antibodies were readily detected in swine serum samples. The EA H1N1 subtype has become dominant in the pig population. The occurrences of coinfections from two or more subtypes afforded opportunities for their reassortment to produce new viruses. Our findings emphasized the need for continuous surveillance of influenza viruses.

show abstract

Evidence for the circulation of genotype 4 Eurasian avian-like lineage swine H1N1 influenza A viruses on Korean swine farms, obtained using a newly developed one-step multiplex RT-qPCR assay

Moon,

Na,

Shin

et al. 2023

Arch Virol

View full text Add to dashboard Cite

Pathogenicity of novel reassortant Eurasian avian-like H1N1 influenza virus in pigs

Cited by 7 publications

References 47 publications

Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Serological Surveillance of the H1N1 and H3N2 Swine Influenza A Virus in Chinese Swine between 2016 and 2021

Evidence for the circulation of genotype 4 Eurasian avian-like lineage swine H1N1 influenza A viruses on Korean swine farms, obtained using a newly developed one-step multiplex RT-qPCR assay

Contact Info

Product

Resources

About