Pathogenicity of some hypocrealean fungi to adult Aedes aegypti (Diptera: Culicidae)

Leles, Renan Nunes; Sousa, N.A.; Rocha, Luiz F.N.; Santos, Adelair Helena dos; Silva, Heloisa Helena Garcia; Luz, Christian

doi:10.1007/s00436-010-1991-y

Cited by 31 publications

(20 citation statements)

References 13 publications

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“…pemphigi from Cerrado soils in Central Brazil attacked the Chagas disease vector, Triatoma infestans . M. anisopliae sensu lato IP 46 has ovicidal activity against Aedes aegypti and also infected adults of both A. aegypti and Anopheles gambiae . The broad insect host ranges of such fungi underscore their potential usefulness for development as mycoinsecticides against insect pests that are agriculturally important as well as against major vectors of human diseases.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Metarhizium spp. in Central Brazilian soils

et al. 2012

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The biodiversity of entomopathogenic fungi in tropical ecosystems is still little investigated, and the objective of this study was to isolate and identify fungi of the entomopathogenic genus Metarhizium (Hypocreales: Clavicipitaceae) present in undisturbed soils of the Central Brazilian Cerrado. A total of 107 Metarhizium isolates was obtained from soils collected from Cerrado sites in the state of Goiás; gene sequences from 63 of these were obtained and compared. Among these, one was confirmed to be M. anisopliae sensu stricto; 53 were very closely allied to M. anisopliae but require more extensive genetic characterization to determine if they might represent a new taxon in the M. anisopliae species complex. Eight of these Cerrado isolates were referable to M. robertsii, and the remaining isolate is the first South American (and Southern Hemisphere) collection of M. flavoviride var. pemphigi. These findings underline the need for better characterization of the diversity of these widely distributed fungi in Brazil.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Metarhizium spp. in Central Brazilian soils

et al. 2012

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“…Aedine eggs are not affected by the most common chemical or biological insecticides (Pérez et al 2007). Metarhizium anisopliae is a well-known entomopathogenic fungus that frequently occurs in soils, and in laboratory conditions M. anisopliae strain IP 46, which was isolated from a soil sample collected in Central Brazil, can attack all stages of A. aegypti, including the eggs (Silva et al 2004;Luz et al 2007;Santos et al 2009;Leles et al 2010). This fungal species is a promising agent for integrated mosquito control (Mnyone et al 2009;Leles et al 2010;Luz et al 2011), and there is need for a better understanding of its effectiveness and applicability in order to develop specific mycoinsecticides against dengue vectors.…”

Section: Introductionmentioning

confidence: 99%

Effects of Metarhizium anisopliae conidia mixed with soil against the eggs of Aedes aegypti

2011

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The effectiveness of Metarhizium anisopliae IP 46 conidia mixed with soil was tested against Aedes aegypti eggs. Mycelium and new conidia developed first on eggs between 4.8 and 15 days respectively after incubation of fungus-treated soils at 3.3 × 10(3) up to 3.3 × 10(5) conidia/g soil at 25°C and relative humidities close to saturation. After 15-day incubation, 53.3% of the eggs exposed to soil with 3.3 × 10(5) conidia/g showed external development of mycelium and conidia. Fungus-inoculated soils (but not untreated controls) showed some mycelial growth and sporulation apart from the eggs. Some eggs on treated soils hatched; those larvae died and eventually showed fungal development on their bodies. The cumulative relative eclosion of larvae after submersion of treated eggs in water decreased from 52.2% at 3.3 × 10(3) conidia/g to 25.3% at 3.3 × 10(5) conidia/g. These findings clearly showed that A. aegypti eggs can be infected by M. anisopliae when deposited on fungus-contaminated soil. The effectiveness of M. anisopliae against gravid females, larvae, and also eggs of A. aegypti underscored the possible usefulness of this fungus as a mycoinsecticide, whether naturally occurring or artificially applied, in the breeding sites of this mosquito.

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“…Larvicidal and adulticidal activity of the entomopathogenic fungus Metarhizium anisopliae is well established against both aedine (Silva et al 2004;Scholte et al 2007;Paula et al 2008Paula et al , 2011Pereira et al 2009;Leles et al 2010) and anopheline mosquitoes (Scholte et al 2004;Mnyone et al 2009Mnyone et al , 2011Bukhari et al 2011). Ovicidal activities by this or other fungi, however, have been demonstrated only recently against Aedes aegypti and Anopheles spp (Luz et al 2007(Luz et al , 2011Leles et al 2012).…”

Section: Introductionmentioning

confidence: 99%

New insights on the effectiveness of Metarhizium anisopliae formulation and application against Aedes aegypti eggs

Sousa

Lobo

Rodrigues

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: The entomopathogenic fungus Metarhizium anisopliae has been extensively studied as a potential agent for biological mosquito control. This study demonstrates the potential of M. anisopliae s.l. strain IP 46, which can infect adults, larvae and eggs of Aedes aegypti, against the eggs of this important vector species by indirect applications of oil-based conidial formulations. AbstractIncreasing needs for innovative control tools against the dengue vector Aedes aegypti have prompted investigations into the development of specific mycoinsecticides. The entomopathogenic fungus Metarhizium anisopliae attacks both larval and adult stages, but its ovicidal activity against A. aegypti is still little explored. This study reports important findings about the effectiveness of conidia formulated in water and oil-in-water emulsions and of direct and indirect application techniques against A. aegypti eggs. The ovicidal activity of M. anisopliae increased with higher conidial concentrations regardless of the application technique, and larvae elimination concentrations were lowest with oil-in-water-formulated conidia (LEC 50 ≤ 4Á8 9 10 3 conidia cm À2 and LEC 90 ≤ 1Á9 9 10 5 conidia cm À2 , respectively). Conidia eventually stimulated larval eclosion. Consequently, the indirect application of oil-based fungal formulations onto substrates where oviposition will later occur appears to be a more efficient means to infect those eggs than the direct fungal application to previously deposited eggs.

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Pathogenicity of some hypocrealean fungi to adult Aedes aegypti (Diptera: Culicidae)

Cited by 31 publications

References 13 publications

Occurrence of Metarhizium spp. in Central Brazilian soils

Occurrence of Metarhizium spp. in Central Brazilian soils

Effects of Metarhizium anisopliae conidia mixed with soil against the eggs of Aedes aegypti

New insights on the effectiveness of Metarhizium anisopliae formulation and application against Aedes aegypti eggs

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