Pathogenicity profile of Vibrio parahaemolyticus in farmed Pacific white shrimp, Penaeus vannamei

Raja, R. Ananda; Sridhar, R.; Balachandran, C.; Palanisammi, A.; Ramesh, S.; Nagarajan, K.

doi:10.1016/j.fsi.2017.06.020

Cited by 105 publications

(47 citation statements)

References 72 publications

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“…In fact, the prebiotic GOS supported both the selected probiotic and other groups of bacteria but not endemic pathogenic bacteria, such as Photobacterium or Vibrio , in this study. Both Photobacterium and Vibrio were predominant during the postlarval stage (80%) and during the juvenile stages (89.1%~94.2%) (Huang et al, ) and can cause serious mortality in shrimp (Ananda et al, ; Liu, Cheng, Hsu, & Chen, ; Liu, Liu, & Li, ). In this study, Photobacterium was dominant at 54.4% and 26.7% in control and SYN‐fed shrimp, respectively, whereas Vibrio species were at 16.7% and 8.5% for control and SYN‐fed shrimp, respectively.…”

Section: Discussionmentioning

confidence: 99%

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Huynh

Chiu

et al. 2019

Aquac Res

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The aims of this study were to unravel the intestinal microbiota of Litopenaeus vannamei after being fed a diet without (control) or with the synbiotic (SYN) for 60 days using next-generation sequencing technology to see if changes in the intestinal microbiota were involved in the improved growth performance and health status of the shrimp. Next-generation sequencing data showed that six phyla, 11 classes, 19 orders, 30 families, 58 genera and 73 species with taxonomic names assigned were detected. The majority of the operational taxonomic units (OTUs) was shared between the SYN and control shrimp and comprised 37 OTUs. However, intestinal biodiversity analyses revealed that SYN-fed shrimp had a higher species richness, evenness and Shannon-Weaver index than did shrimp fed the control diet, but without reaching statistical significance. Interestingly, shrimp fed the SYN diet exhibited improved colonization of Lactobacillus plantarum and reduced prevalences of Vibrio harveyi and Photobacterium damselae in the intestines. These findings indicate that the SYN was able to modulate the intestinal bacterial community of shrimp and could be used to control vibriosis in shrimp. K E Y W O R D Sgalactooligosaccharide, intestinal microbiota, Lactobacillus plantarum, Litopenaeus vannamei, synbiotic

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Section: Discussionmentioning

confidence: 99%

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Huynh

Chiu

et al. 2019

Aquac Res

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“…The feeding trial was conducted for 42 days under laboratory condition in fibre reinforced plastic tank (Length × Breadth × Height: 0.65 × 0.50 × 0.48 m) containing dechlorinated brackishwater with continuous aeration. Hatchery‐bred and PCR tested healthy P. monodon (mean individual weight 2.73 ± 0.01 g) were randomly distributed in tanks with 10 shrimps per tank for three groups in triplicates (Ananda Raja et al., ). Groups I, II and III were fed with diets D1, D2 and D3 respectively.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of growth, feed utilization efficiency and immune parameters in tiger shrimp (Penaeus monodon) fed diets supplemented with or diet fermented with gut bacteriumBacillussp. DDKRC1. isolated from gut of Asian seabass (Lates calcarifer)

Raja

Ghoshal

et al. 2018

Aquac Res

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Evaluation of growth, feed utilization efficiency and immune parameters in tiger shrimp (Penaeus monodon) fed diets supplemented with or diet fermented with gut bacterium Bacillus sp. DDKRC1. isolated from gut of Asian seabass (Lates calcarifer) SummaryAn experiment was conducted to assess the effect of supplementation of cellulolytic gut bacterium with plant protein-based diet on growth, digestibility and digestive enzyme status of tiger shrimp, Penaeus monodon. Bacillus sp. DDKRC1. (JN641289), a potential cellulolytic bacterium, isolated from the gut of Asian seabass was used as feed supplement in this experiment. The shrimps of group I, II and III were fed with control diet (without bacterial supplement, D1), diet supplemented with live Bacillus sp. DDKRC1. at 2.94 9 10 7 cfu per 100 g feed (D2) and diet fermented (48 hr) with same bacteria (D3) respectively. At the end of the 42 days experiment, shrimps of group II showed significantly better (p < .01) growth and protein efficiency ratio (PER) and lower (p < .01) feed conversion ratio (FCR) as compared to group I and III. Dry matter, cellulose, hemicellulose and lipid digestibilities were significantly (p < .01) higher in group II than other two groups. Cellulase, amylase and protease activities in the gastrointestinal (GI) tract were significantly (p < .01) higher in group II as compared to other two groups. Total heterotrophic count, amylolytic, cellulolytic and proteolytic bacterial counts in gut were significantly (p < .01) higher in group II as compared to other two groups. Haematological analysis indicated better (p < .05) immune response in groups II and III than control group. Diets D2 and D3 showed better effect on growth, digestibility, FCR, survival and immune response of P. monodon as compared to D1.

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“…Of them, vibriosis is usually considered to be the most common and significant bacterial disease (Defoirdt, Boon, Sorgeloos, Verstraete, & Bossier, 2007). Many Vibrio species have been well documented as pathogens causing vibriosis for shrimp, such as V. harveyi (Rungrassamee, Klanchui, Maibunkaew, & Karoonuthaisiri, 2016; Soto‐Rodriguez et al, 2012), V. alginolyticus (Lee, Yu, Yang, Liu, & Chen, 1996; Vandenberghe et al, 1999), V. parahaemolyticus (Ananda Raja et al, 2017; Tran et al, 2013), V. campbellii (Soto‐Rodríguez, Simoes, Roque, & Gómez Gil, 2006), V. nigripulchritudo (Goarant et al, 2006; Walling, Vourey, Ansquer, Beliaeff, & Goarant, 2010) and V. penaecida (Goarant & Merien, 2006; Maningas, Kondo, Hirono, Saito‐Taki, & Aoki, 2008).…”

Section: Introductionmentioning

confidence: 99%

A novel research on isolation and characterization of Photobacterium damselae subsp. damselae from Pacific white shrimp, Penaeus vannamei, displaying black gill disease cultured in China

Wang

Shi

Wang

et al. 2020

Journal of Fish Diseases

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In June 2019, massive mortalities of cultured Penaeus vannamei occurred in a local farm in Hainan Province, China. The diseased shrimp displayed evident black gills.Three bacterial strains 20190611001, 20190611007 and 20190611022 were isolated from hepatopancreas and gills of the diseased shrimp and identified as Photobacterium damselae subsp. damselae based on the sequence analysis of 16S rRNA and toxR genes. These three isolates showed haemolytic activities. Of them, strain 20190611022 isolated from hepatopancreas was selected and processed for pathogenic analysis. The calculated median lethal dose (LD 50 ) was 9.75 ± 4.29 × 10 5 CFU/g (body weight) by challenging P. vannameivia reverse gavage. The diseased shrimp displayed enlarged hepatopancreatic tubules and sloughing of epithelial cells in tubular lumens. The strain 20190611022 was also characterized by the testing of API 20NE systems and antibiotic susceptibility. The results of disc diffusion test showed that strain 20190611022 was sensitive to chloramphenicol, compound sulfamethoxazole, cefoperazone, ceftriaxone, ceftazidime and cefuroxime. To our knowledge, this is the first report of isolation and characterization of Photobacterium damselae subsp. damselae from natural diseased P. vannamei. Our findings can serve as a basis for further studies of its pathogenicity and provide technological support for disease controlling in shrimp aquaculture. K E Y W O R D Sblack gill disease, pathogenicity, Penaeus vannamei, Photobacterium damselae subsp. damselae

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Pathogenicity profile of Vibrio parahaemolyticus in farmed Pacific white shrimp, Penaeus vannamei

Cited by 105 publications

References 72 publications

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Evaluation of growth, feed utilization efficiency and immune parameters in tiger shrimp (Penaeus monodon) fed diets supplemented with or diet fermented with gut bacteriumBacillussp. DDKRC1. isolated from gut of Asian seabass (Lates calcarifer)

A novel research on isolation and characterization of Photobacterium damselae subsp. damselae from Pacific white shrimp, Penaeus vannamei, displaying black gill disease cultured in China

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