Pathogenicity testing and antimicrobial susceptibility ofHelicobacter pullorumisolates from chicken origin

Abstract

This work aimed to study the pathogenicity and to investigate the antimicrobial susceptibility and resistance patterns of Helicobacter pullorum (H. pullorum). The minimum inhibitory concentration (MIC) value of ciprofloxacin, ampicillin, gentamycin, erythromycin, colistin sulfate and tetracycline was determined for eight different H. pullorum isolates. H. pullorum resulted into 33.3% mortality of infected chickens with signs of diarrhea, stunted growth and poor conversion rate in survivors. All experimentally infected embryonated chicken eggs showed embryonic mortalities within 48-h post yolk sac inoculation. H. pullorum was re-isolated from cecum, liver, yolk sac and air-sacs of all dead and sacrificed infected chickens. H. pullorum was also re-isolated from dead embryos, embryonic membranes and fluids of infected embryonated chicken eggs. Polymerase Chain Reaction (PCR) assay was used to detect H. pullorum in experimentally infected chickens and embryonated chicken eggs. All tested H. pullorum isolates were resistant to ciprofloxacin, gentamycin and erythromycin, while 7 out of 8 isolates were resistant to tetracycline. All isolates were susceptible to colistin sulfate and ampicillin.

Helicobacter pullorum: An Emerging Zoonotic Pathogen

“…It is a motile, non-spore forming, microaerophilic bacterium, which best grows at 37–42°C (Hassan et al, 2014a). H. pullorum produces catalase, reduces nitrates, but lacks urease, indoxyl acetate esterase, or alkaline phosphatase activity.…”

“…Isolates of poultry origin show resistance to ciprofloxacin, gentamycin, erythromycin and tetracyclin and is susceptible to colistin sulfate and ampicillin (Hassan et al, 2014a). Draft genome sequence of H. pullorum human isolate, MIT 98-5489 reveal that the bacteria are clarithromycin resistant.…”

Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern

“…These two groups are considered zoonotic microorganisms (Josenhans et al, 2000). In general, during infection by the gastric Helicobacter group, the bacteria colonize the stomach; the enterohepatic Helicobacter group principally colonizes the distal portion of the digestive system and biliary duct (Hassan et al, 2014). Helicobacter pullorum (H. pullorum) , which was first isolated from the cecum of apparently healthy domestic fowls and the small intestine and liver of fowls with severe gastroenteritis and hepatitis (Stanley et al, 1994, Zanoni et al, 2007, Qumar et al, 2017), is a member of the enterohepatic Helicobacter group.…”

Helicobacter pullorum: A potential hurdle emerging pathogen for public health

“…Although the main niche for colonization of H. pullorum is the intestine especially the caecum, but Hassan et al [16] proved presence of the pathogen also in the liver tissues of the birds. Moreover, the study of Hassan et al [46] demonstrated that out of 900 cloacal, caecal and liver tissues of broiler chickens, the incidence rate of H. pullorum was 39.33% using species-specific 16S rRNA PCR. Experimentally inoculated broilers with H. pullorum elicited 33.3% mortalities with signs of diarrhea, retardation of growth with poor conversion rate and the pathogen was reisolated from the caecum, liver, yolk sac and air-sacs of dead and sacrificed chickens [46].…”

“…Moreover, the study of Hassan et al [46] demonstrated that out of 900 cloacal, caecal and liver tissues of broiler chickens, the incidence rate of H. pullorum was 39.33% using species-specific 16S rRNA PCR. Experimentally inoculated broilers with H. pullorum elicited 33.3% mortalities with signs of diarrhea, retardation of growth with poor conversion rate and the pathogen was reisolated from the caecum, liver, yolk sac and air-sacs of dead and sacrificed chickens [46]. Mohamed et al [45] Out of 900 cloacal, caecal and liver tissues of broiler chickens, the incidence rate of H. pullorum was 39.33%…”