Pathogens in Processed Ram Semen and Approaches for Their Elimination

Savvulidi, Filipp; Ptáček, Martin; Stádník, Luděk

doi:10.11118/actaun201866041065

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Hence, quality of frozen-thawed semen is an important factor that predicts bull fertility and the sustainability of AI as well as investment of bulls with high genetic merits [7]. However, quality of semen can be altered, among others, by the process of cryopreservation [8][9][10][11][12][13], types of extender used [14], and bacterial contamination [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

Reda

Almaw²,

Abreha

et al. 2020

Veterinary Medicine International

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The objectives of this trial were to estimate prevalence of bacteriospermia, to determine the bacterial load, and to isolate the types of bacteria as well as to assess the association between bacterial load and sperm quality traits in cryopreserved bull semen in field conditions in the South Wollo Zone. A total of 309 cryopreserved straws of semen from the Holstein Friesian (HF)-cross bull (n = 180 straws) and pure Jersey bull (n = 129 straws) were investigated. Bacteriological assessments of the presence of aerobic bacteria, estimation of bacterial count and bacterial isolation, as well as semen quality were performed. Aerobic bacterial contamination was prevalent in 38.8% of the semen straws. No significant difference in the prevalence of bacteriospermia was observed among bulls although the HF-cross bull had a higher prevalence (40.0%). But, significant difference in prevalence of bacteriospermia was found among semen ejaculates of the same bull. The risk of bacteriospermia in the HF-cross bull was higher (Odds ratio = 1.86, 95% CI = 0.168–20.26) compared to Jersey although not significant. Overall average bacterial load of 50.38 ± 16.29 colony-forming units (CFU)/ml (from nil to 1318.20 CFU/ml) was found. No significant difference in bacterial count among bulls and their ejaculates was observed. Moreover, correlation analysis revealed that the proportions of motility, live, and normal morphology were negatively influenced by an increase in the bacterial contamination of semen. In this study, three isolates of coagualse-negative Staphylococcus species and one isolate of Corynebacterium species were found. Average percentages of sperm motility (48.35 ± 1.23), live (66.08 ± 1.0), and normal morphology (80.62 ± 1.24) were observed. It was concluded that cryopreservation does not guarantee the quality of semen from bacterial contamination. Hence, meticulous care should be adopted to prevent contamination of semen by bacteria during collection, transportation, processing, and storage times.

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Section: Introductionmentioning

confidence: 99%

Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

Reda

Almaw²,

Abreha

et al. 2020

Veterinary Medicine International

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“…Experimentally infected sheep did not exhibit any clinical signs of infection (fever, abortion, stillbirth) when tested. T. gondii can also be sexually transmitted through semen (de Moraes et al 2010;Lopes et al 2013), with infected rams becoming infertile (Savvulidi et al 2018).…”

Section: Toxoplasmosis In Wild Animalsmentioning

confidence: 99%

Warm-blooded animal toxoplasmosis and its connection to COVID-19: A review

Bakhraibah

2022

MBJ

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Toxoplasmosis is a zoonotic illness that spreads from animals to people. Toxoplasma gondii, a protozoan parasite that infects warm-blooded mammals, causes the sickness. Toxoplasmosis is a parasitic infection that causes abortion and death in animals. Cats are the parasite's sole sexual hosts, thus they're the only ones who can get it. Because cats are frequent pets, they are highly likely to come into touch with humans. As a result, the disease poses a risk to human health. The potential danger is influenced by the frequency of oocyst secretion and the level of contamination in the environment. Toxoplasmosis has serious consequences for both animal and human health, hence preventative actions should be taken to reduce the dangers. COVID-19 is affected by such methods as well. Toxoplasmosis is thought to increase immunological and immunosuppressive factors, which increases the chance of SARS-CoV-2 infection and the severity of the resulting COVID-19. Research into Toxoplasma gondii intermediate hosts might help understand COVID-19's dynamics and determine if the virus can be transferred from animals to humans. We explore what we know about Toxoplasma gondii infection as a human parasitosis and how it may alter the course of SARS-CoV-2 infection in this review study.

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“…Infections and subsequent inflammation in the male genital tract may adversely influence the sperm cell function and the entire spermatogenetic activity (Fraczek & Kurpisz, 2015; Lu et al., 2013), and may cause qualitative and quantitative sperm transformations. Many microbial species may survive in the semen even after cryopreservation (Savvulidi et al., 2018). Retrospective studies have shown that merely presence of bacteria in semen samples may compromise the sperm fertilising ability.…”

Section: Introductionmentioning

confidence: 99%

Semen quality and total microbial load: An association study in important Indian Goat breeds during different seasons

et al. 2021

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The invasion of the male urogenital tract by microorganisms, and its subsequent effects on sperm fertilising ability, has not been well discussed in bucks. The present study was conducted to assess the bacterial load in fresh semen of the 2–6 years old bucks. For conducting the experiment, semen ejaculates from 18 bucks (6 from each breed namely Jakhrana, Jamunapari and Barbari) were used. We collected 5 ejaculates from each buck in each season (Summer‐April to June, Rainy‐July to Sept and Winter‐November to January). Semen was collected with the artificial vagina (AV) method, and separate AV was used for each buck every time. The semen collection frequency was once in a week. Immediately after initial evaluation, collected semen samples were transferred to the microbiology laboratory of the institute. Thereafter, the semen samples were subjected to bacteriological examination to assess the microbial load. The results of the current study indicate that the microbial load in the semen was significantly (p < 0.05) higher in the Jamunapari bucks and in aged bucks. Bacteriospermia in different seasons was not significantly varied; however, nonsignificant increase in microbial load during the rainy season was observed. Overall, the average bacterial load in the semen of Jamunapari, Barbari and Jakhrana bucks was found 540.50 ± 55.88 CFU/ml, 391.81 ± 46.33CFU/ml and 388.93 ± 44.71 CFU/ml respectively. No significant difference in bacterial counts in the subsequent ejaculates among bucks was observed. Moreover, correlation analysis revealed that the proportions of motility, viability, plasma membrane integrity and acrosomal integrity were negatively influenced by the increased bacterial contamination of buck semen.

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Pathogens in Processed Ram Semen and Approaches for Their Elimination

Cited by 5 publications

References 32 publications

Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

Warm-blooded animal toxoplasmosis and its connection to COVID-19: A review

Semen quality and total microbial load: An association study in important Indian Goat breeds during different seasons

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