Bone mineral density (BMD, g/cm 2 ) in the distal portion of the third metacarpal bone (McIII) of racehorses was measured by a dual energy x-ray absorptiometry system (DEXA; ALOKA Co, Ltd., Tokyo, Japan) Although there are several techniques to measure bone mineral content in vivo or in vitro, photon absorptiometry using 241 Americium as a source of monoenergetic (60 KeV) photons (SPA) has been used in horses [5]. SPA has reported that the value of the McIII measured by SPA were well correlated with the weight of ashed McIII, and the reproducibility of the value was high, thus being a precise and accurate non-invasive in vitro method of quantifying bone mineral content in equine McIII [5].Dual energy x-ray absorptiometry (DEXA), which is a relatively new photon absorptiometry, uses two energies of x-rays as the photon source. Unlike SPA, DEXA avoids the needs for the limb to be immersed or surrounded by soft-tissue-equivalent-medium, provides images not available by SPA (so that a region of interest can be set in a fixed position while observing images and ensuring the position) and has been reported to be a quick and reliable method for determining bone mineral content [3]. The principle of the two techniques described above is to scan a bone with a narrow beam of singleenergy photons in SPA or dual-energy photons in DEXA and measure the degree of attenuation of the beam by bone, relative to its attenuation by tissue, by means of a detector system. A direct relationship is established between the number of extra photons absorbed and bone mineral content. The objective of this study was to investigate the correlation of bone mineral content measured by SPA and that determined by DEXA for applying DEXA as the method of quantifying mineral content in vitro of the distal portion of McIII in racehorses.Measurements of mineral contents of the McIII in racehorses was made using DEXA (DCS-3000; ALOKA