Pathological and Immunohistochemical Study of the Liver and Hepatic Lymph Nodes of Sheep Chronically Reinfected withFasciola hepatica , with or without Triclabendazole Treatment

Pérez, J.; Ortega, J.; Moreno, T.; Morrondo, P.; López-Sández, Ceferino; Martı́nez-Moreno, A.

doi:10.1053/jcpa.2002.0561

Cited by 33 publications

(39 citation statements)

References 19 publications

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“…The results of the primary trickle-infected and treated lambs (group 4) indicate that TCBZ therapy administered at 12 wpi is effective in eliminating parasites and in significantly reducing the hepatic inflammatory infiltrate by 30 wpi, as previously reported [24]. However, in this study the use of TCBZ during the migratory stage of the parasites (group 5) did not induce significant protection against future infections in sheep as indicated by the number of hepatic parasites and the heavy hepatic infiltrate of all cell subsets found in this group.…”

Section: Discussionsupporting

confidence: 78%

“…In a previous study, we found that trickle infections (either primary or challenge) induced more severe hepatic damage than infections with two larger doses in sheep [24]. The present study revealed a higher hepatic inflammatory infiltrate of T cell subsets (CD3 + , CD4 + and CD8 + ), CD79α + B cells and IgG bearing plasma cells in trickle-infected lambs (group 2) than in the single-dose challenge lambs (group 1).…”

Section: Discussionsupporting

confidence: 58%

“…The anti-CD3 polyclonal antibody (pAb) was used as a pan-T cell marker, and was shown to cross-react with ovine T lymphocytes [26]. A rabbit anti-human IgGlambda light chain (pAb), which cross-reacts with sheep IgG [24], was used to label IgG. An anti-human CD79α mAb, which crossreacts with B cells of sheep [21], was used as a pan-B cell marker.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…More recently, the effects of infection and treatment on subsequent challenge have been studied in cattle [13]. Our group studies the effects of small repetitive infections (trickle infection), which are similar to those that often occur in natural fasciolosis [7], with and without treatment upon subsequent challenges in sheep [24].…”

Section: Introductionmentioning

confidence: 99%

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Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Pérez¹,

Ortega²,

Bravo³

et al. 2005

Vet. Res.

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-The phenotype of the hepatic inflammatory infiltrate and hepatic lymph nodes (HLN) was analysed in lambs primarily and challenge infected with Fasciola hepatica. Group 1 was primarily and challenge infected with two doses of 200 metacercariae (mc) each and was nontreated. Trickle infection was administered to five groups: group 2 was challenge infected and nontreated; group 3 was primarily infected and non-treated; group 4 was primarily infected and treated with triclabendazole (TCBZ) at 12 weeks postinfection (wpi); group 5 was treated at 4 wpi and challenge infected and group 6 was treated at 12 wpi and challenge infected. An uninfected group was used as the control. The distribution of T cell subpopulations (CD3, CD4 and CD8), and B cells (CD79α, IgM, IgG) was analysed. The hepatic inflammatory infiltrate was represented mainly by CD3 and CD4 T cells, and B cells (CD79α, IgG). These infiltrates were more severe (P < 0.05) in primarily (group 3) or challenge (groups 2, 5 and 6) trickle infected lambs than in the group single challenge infected (group 1). Cellular changes in HLN consisted in an increase of CD4 over CD8 T cells and an increase of B cells and IgG + plasma cells, and they were more severe in primarily and challenge trickle infected groups than in the group infected with two larger doses of mc, although significant differences were not found with respect to all challenge trickle infected groups. The strong local cellular and humoral immune responses did not protect against subsequent infections, neither in non-treated lambs (group 2) nor in lambs treated with TCBZ at 4 wpi (group 5) or 12 wpi (group 6).

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 58%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Pérez¹,

Ortega²,

Bravo³

et al. 2005

Vet. Res.

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“…In lambs experimentally infected with D. dendriticum data relative to egg elimination , anti-D. dendriticum antibody (Ab) kinetics , hepatic enzymes, biochemical parameters and pathological lesions (Manga- González et al 2004) have been reported. Different studies have evaluated the cellular and humoral immune responses, using immunohistochemical criteria, against Fasciola hepatica after experimental infections in different hosts: sheep (Meeusen et al 1995;Chauvin and Boulard 1996;Pérez et al 2002Pérez et al , 2005, goats (Pérez et al 1998Martínez-Moreno et al 1999) and rats (Tliba et al 2000). F. hepatica-infected alpacas are also known to have elicited a stronger humoral response to the Fas2 parasite antigen (Timoteo et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical study of the local immune response in lambs experimentally infected with Dicrocoelium dendriticum (Digenea)

et al. 2007

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Phenotypic expression of inflammatory cells in liver and hepatic lymph nodes (HLN) has been examined in lambs experimentally infected with Dicrocoelium dendriticum using immunohistochemical techniques. Thirty-two lambs, 12 infected with 1,000 D. dendriticum metacercariae, 12 with 3,000, and 8 controls were used. Half the lambs in each group were slaughtered on days 60 and 180 post-infection (p.i.), respectively. Primary antibodies (Abs) against T cell epitopes (CD3+, CD4+, CD8+ and WC1+ gammadelta), B cell epitopes (CD79alphacy+, CD45R+), immunoglobulin (IgG)-bearing plasma cells, macrophages (CD14+, VPM32+) and major histocompatibility complex (MHC) class IIbeta antigen were used. T lymphocytes (CD3+, CD4+ and CD8+) and B lymphocytes (CD79alphacy+ and CD45R+) with diffuse pattern or forming lymphoid aggregates and follicles surrounded the septal bile ducts (SBD) and inter-lobular bile ducts, whereas the WC1 gammadelta T cells were scattered. Numerous IgG+ plasma cells were observed around SBD. CD14 and VPM32+ macrophages intermingled with lymphocytes were immunostained by the anti-MHC class IIbeta. This Ab also reacted with lymphoid cells. Likewise, increased positive immunostaining for all Abs used was observed in the HLN of infected lambs. There was no qualitative difference regarding the phenotype expression of inflammatory cells between the lambs infected with D. dendriticum. The humoral and cell-mediated local immune responses observed were similar in the two groups of lambs infected with different doses.

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Hepatobiliary Platynosomosis in Black‐Tufted Marmosets (Callithrix penicillata): A Lethal Threat for Wildlife and Captive Populations

de Macêdo,

de Sousa,

Souza

et al. 2024

American J Primatol

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Helminthic infections, particularly those caused by trematodes, pose a significant health risk to both animals and humans. This study investigates hepatobiliary platynosomosis (HP) caused by Platynosomum illiciens in black‐tufted marmosets (Callithrix penicillata) in Central Brazil. Data were retrospectively collected from autopsy records at the Laboratory of Veterinary Pathology and Forensics, University of Brasília, from January 2006 to July 2021. Epidemiological, clinical, and pathological information was analyzed, including comparisons between free‐ranging and captive marmosets. A total of 1596 nonhuman primate (NHP) death records were examined, with black‐tufted marmosets accounting for 75.6% (1206/1595) of autopsies. HP was identified in 10.8% (131/1206) of these cases. Captive marmosets showed a higher prevalence of HP (49.6%) than free‐ranging ones (6.5%). This study revealed a significant seasonal trend, with higher HP prevalence observed during the wet season until the onset of the dry season. Pathological examinations revealed severe liver and bile duct damage in fatal HP cases, including fibrosis, bile duct thickening, and the presence of flukes. Captive marmosets exhibited pronounced clinical signs, such as weight loss and apathy. Morphological and molecular analysis of sampled flukes confirmed P. illiciens infecting the marmosets. These findings underscore the substantial impact of HP on marmoset populations, particularly in captive settings. The high lethality (58% overall; 81.6% in captivity) highlights the need for improved management and preventive measures in captive environments. This study contributes to understanding HP epidemiology, clinical manifestations, and pathological outcomes, underscoring the disease's significance for the health of both free‐ranging and captive NHP populations. Our findings also support the need to develop targeted strategies to mitigate the impact of HP on primate species populations.

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Pathological and Immunohistochemical Study of the Liver and Hepatic Lymph Nodes of Sheep Chronically Reinfected withFasciola hepatica , with or without Triclabendazole Treatment

Cited by 33 publications

References 19 publications

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Immunohistochemical study of the local immune response in lambs experimentally infected with Dicrocoelium dendriticum (Digenea)

Hepatobiliary Platynosomosis in Black‐Tufted Marmosets (Callithrix penicillata): A Lethal Threat for Wildlife and Captive Populations

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