Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice

Saminathan, Mani; Maity, Madhulina; Vineetha, S.; Manjunathareddy, Gundallhalli Bayyappa; Dhama, Kuldeep; Malik, Yashpal Singh; Ramakrishnan, Muthannan Andavar; Misri, Jyoti; Gupta, Vivek Kumar

doi:10.1016/j.jare.2021.01.007

Cited by 11 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 The histopathological lesions were marked necrosis in tunica media of pulmonary artery and aorta, congestion, mononuclear cells infiltration, capillary damage with haemorrhages and oedema, and perivascular cuffing seen in this study, which can be correlated to the earlier studies. 28 Further, in this study on 7 dpi, endothelial cells of the major vessels were found swollen, which may be due to hypertrophy leading to sloughing of these cells into the lumen of arteries, which can be related to the study done by Saminathan et al 30 Presence of BTV antigen in the arteries was confirmed by immunohistochemistry and RT-PCR. As per the previous studies for locating the BTV antigen in blood vessels, it was seen that endothelial cells of capillaries and arterioles were mostly associated with the antigen.…”

Section: Discussionsupporting

confidence: 74%

See 1 more Smart Citation

Understanding the Pathogenesis of Endothelial Injury Induced by Bluetongue Virus in Experimentally Infected Sheep

Singh,

Saminathan,

Dinesh

et al. 2024

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Bluetongue (BT) is an economically important contagious viral disease transmitted by insect vectors of Culicoides species affecting both domestic and wild ruminants caused by Genus Orbivirus of Reoviridae family. Present in vivo study for the first time conducted to understand the role of oxidative stress and acute phase proteins in the pathogenesis of endothelial injury in target blood vessels of BTV-4 infected sheep. Twelve sheep of either sex were divided into two groups of six animals each and infected intradermally at multiple sites in the neck region with 6×105.9 TCID50/ml of BTV-4 and animals of control group were inoculated with uninfected tissue culture fluid. Animals were sacrificed at 3, 7, 15, 28, 60, and 90 days post infection (dpi) and postmortem examination was conducted. Oxidative stress parameters like LPO, SOD, GPO and catalase in the blood vessels, hemolysate and serum were estimated. Oxidative stress parameter like LPO was upregulated and anti-oxidant parameters like SOD and GPO were downregulated in blood vessels homogenates, hemolysate and serum of BTV-4 infected animals than uninfected control. This shows the role of oxidative stress in causing endothelial cell damage during BTV infection. Acute phase proteins like CRP and fibrinogen in serum and plasma were estimated and found upregulated in BTV-4 infected animals, which shows that inflammatory changes during the disease causes vascular damage. Prothrombin time was estimated in plasma of BTV-4 infected animals and was prolonged in BTV infected animals indicating coagulation disorder. Increased oxidative stress and fibrinogen, and prolonged prothrombin time indicated the coagulation disorders induced by BTV infection by causing endothelial cell damage and severe vascular disease results in congestion, haemorrhages and edema in the blood vessels and other target organs in sheep.

show abstract

Section: Discussionsupporting

confidence: 74%

“…As per the previous studies for locating the BTV antigen in blood vessels, it was seen that endothelial cells of capillaries and arterioles were mostly associated with the antigen. 30 In the present study, larger arteries like aorta and pulmonary artery were found positive for BTV antigen by immunohistochemistry.…”

Section: Discussionsupporting

confidence: 61%

Understanding the Pathogenesis of Endothelial Injury Induced by Bluetongue Virus in Experimentally Infected Sheep

Singh,

Saminathan,

Dinesh

et al. 2024

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Notwithstanding other virulence factors, the differential virulence observed in cattle between the viral isolates used in this work could be partly explained by a differential immunomodulation of the host IFN response, which eventually might or not restrict host range. Although it can help explain the inability of EHDV-8 to infect A129 mice, the differential immunomodulation of the host IFN response cannot explain our results in IFNAR(-/-) mice, whose susceptibility to other orbiviruses such as BTV depends on an impaired IFN-I response 50 , 52 . Therefore, other virulence determinants must be involved in the differential virulence between these viruses.…”

Section: Discussionmentioning

confidence: 79%

IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

Jiménez-Cabello,

Utrilla-Trigo,

Benavides-Silván

et al. 2024

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Epizootic hemorrhagic disease (EHD), caused by Epizootic hemorrhagic disease virus (EHDV), is an emerging and severe livestock disease. Recent incursion and distribution of EHDV in Europe have outlined the emerging character of EHD. Despite its worldwide impact, numerous knowledge gaps exist. A range of inconveniences restricts utilization of natural hosts of EHDV. Here, we show that adult mice deficient in type I IFN receptor (IFNAR(-/-)) are highly susceptible to EHDV-6 and EHDV-8 infection when the virus is administered subcutaneously. Disease was characterized by ruffled hair, reluctance to move, dehydration and conjunctivitis, with viraemia detected from day 5 post-infection. A deeper characterization of EHDV-8 infection showed viral replication in the lung, liver, spleen, kidney, testis and ovaries. Importantly, increased expression levels of pro-inflammatory cytokines IL-1β, IL-6 and CXCL2 were observed in spleen after EHDV-8 infection. Furthermore, IFNAR(-/-) adult mice immunized with a EHDV-8 inactivated vaccine elicited neutralizing antibodies specific of EHDV-8 and full protection against challenge with a lethal dose of this virus. This study also explores the possibilities of this animal model for study of BTV and EHDV coinfection. In summary, the IFNAR(-/-) mouse model faithfully recapitulates EHD and can be applied for vaccine testing, which can facilitate progress in addressing the animal health challenge posed by this virus.

show abstract

“…Interestingly, BTV-related leukopenia has also been reported in interferon deficient mouse models of infection ( Saminathan et al. 2020 b; Saminathan et al., 2021 ). This immune suppression is thought to be driven by follicular dendritic cells as was observed in a sheep model of BTV-8 infection ( Melzi et al., 2016 ).…”

Section: Discussionmentioning

confidence: 95%

Monitoring longitudinal immunological responses to bluetongue virus 17 in experimentally infected sheep

Westrich,

McNulty,

Carpenter

et al. 2023

Virus Research

View full text Add to dashboard Cite

Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice

Abstract: Graphical abstract

Cited by 11 publications

References 46 publications

Understanding the Pathogenesis of Endothelial Injury Induced by Bluetongue Virus in Experimentally Infected Sheep

Understanding the Pathogenesis of Endothelial Injury Induced by Bluetongue Virus in Experimentally Infected Sheep

IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

Monitoring longitudinal immunological responses to bluetongue virus 17 in experimentally infected sheep

Contact Info

Product

Resources

About