2021
DOI: 10.18805/ijar.b-1301
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Pathological and Molecular Characterization of Newcastle Disease Virus Isolated from Gallus gallus in Java, Indonesia

Abstract: Background: Known as the vital avian disease in Indonesia, Newcastle disease (ND) has surged all over Indonesia which cause an immense drop in poultry industry. This investigation intended to examine the pathological and molecular characteristics of the latest Newcastle disease virus (NDV) derived from Gallus gallus in Java, Indonesia.Methods: The samples were inoculated on the embryonic chicken eggs (ECEs), then identified using the hemagglutination test (HA test), insulated isothermal PCR (iiPCR) and hemaggl… Show more

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Cited by 2 publications
(4 citation statements)
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“…Quantitative and qualitative test of DNA isolation 1.5% agarose gel electrophoresis in 1XTAE solution was used for a quantitative and qualitative evaluation of the extracted DNA. For 40 minutes, electrophoresis was carried out at 100 V. By using spectrophotometry on all samples, purity level measurement and concentration of the extracted DNA are achieved (Ansori et al, 2021).…”
Section: Blood Samples Collection and Dna Extractionmentioning
confidence: 99%
See 2 more Smart Citations
“…Quantitative and qualitative test of DNA isolation 1.5% agarose gel electrophoresis in 1XTAE solution was used for a quantitative and qualitative evaluation of the extracted DNA. For 40 minutes, electrophoresis was carried out at 100 V. By using spectrophotometry on all samples, purity level measurement and concentration of the extracted DNA are achieved (Ansori et al, 2021).…”
Section: Blood Samples Collection and Dna Extractionmentioning
confidence: 99%
“…The amplification conditions were as follows initial denaturation (95C for 5 mins), denaturation (95C for 45 sec with 35 cycles), annealing (60C for 45 sec), extension (72C for 60 sec) and final extension (72C for 5 mins). The PCR products were sequenced employing ABI 310 xL Genetic Analyser (Applied Biosystems, Inc) referred to Ansori et al (2021) and Prihandini et al (2022).…”
Section: Pcr Amplificationmentioning
confidence: 99%
See 1 more Smart Citation
“…Shrimp white spot syndrome virus (WSSV) [303] Mayaro virus (MAYV) [304,305] 2 Sin Nombre virus (SNV) [306] Newcastle disease virus (NDV) [307,308] 2 Smallpox viruses [309] Respiratory syncytial virus (RSV) [310,311] 2 Tick-borne encephalitis virus (TBEV) [312] Rift Valley fever virus [313,314] 2 Yellow fever virus (YFV) [315] Boldface denotes the top three pathogens with the highest number of publications. The table is sorted by number of publications descending, then by pathogen ascending.…”
Section: Analysis Of Publications By Pathogenmentioning
confidence: 99%