Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis

Hsieh, Chi-Ying; Chang, Tsung-Chou; Shen, Yuqiang; Chang, Chao-Pei Betty; C, Tu; Tung, Ming-Chen; Chen, L.C.; Tsai, Shinn-Shyong

doi:10.1016/j.aquaculture.2006.07.005

Cited by 8 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This agent might be transmitted via the oral or respiratory route and spread hematogenously or lymphogenously throughout the body and induced systematic infection which was characterized by visceral granuloma formation in the various organs such as the lung, liver, spleen and intestine. In the turtle, some systematic granulomatous disease due to M. chelonei, M. marinum and M. kannsasii and osteomyelitis due to M. chelonae have been reported [5,7,12,15]. It is difficult to identify the particular pathogen, because most of them may form the histopathological features similar to our case.…”

contrasting

confidence: 39%

Mycobacterium ulcerans Infection in an Indian Flap-Shelled Turtle (Lissemys punctata punctata)

et al. 2011

View full text Add to dashboard Cite

ABSTRACT. We report an atypical mycobacterial infection in an Indian flap-shelled turtle, Lissemys punctata punctata, that died in an aquarium in Japan. At necropsy, the turtle showed multiple white nodules on the capsular surface and parenchyma of various organs such as the liver, spleen, intestine, and lung. Histologically, granulomatous inflammation surrounding a central zone of necrosis was observed. Sections stained by the Ziehl-Neelsen method revealed numerous acid-fast bacilli in the cytoplasm of macrophages and in the central area of necrosis. The organisms were identified as a mycobacterial species by PCR and nucleotide sequence analysis and revealed 98-100% homology to M. ulcerans. This is, to our knowledge, the first report of mycobacteriosis due to M. ulcerans in a turtle.KEY WORDS: Indian flap-shelled turtle, Mycobacterium ulcerans infection, PCR.

show abstract

contrasting

confidence: 39%

Mycobacterium ulcerans Infection in an Indian Flap-Shelled Turtle (Lissemys punctata punctata)

et al. 2011

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…Generally, disease occurs when turtles are cultured at high density as a result of overstocked ponds or when turtles are exposed to poor water quality. In recent years, bacterial disease has increased the difficulty of aquaculture management and has led to limited yields, often resulting in significant economic loss [1][2][3]. In 2011, several cases of Chinese soft-shell turtle disease originating from B. cereus infection were reported for the first time in the pond-cultured farms in Guangdong Province, China [4].…”

Section: Introductionmentioning

confidence: 99%

Retrospective Screening of Anthrax-like Disease Induced by Bacillus tropicus str. JMT from Chinese Soft-Shell Turtles in Taiwan

2023

View full text Add to dashboard Cite

Bacillus cereus is ubiquitous in the environment and a well-known causative agent of foodborne disease. Surprisingly, more and more emerging strains of atypical B. cereus have been identified and related to severe disease in humans and mammals such as chimpanzees, apes, and bovine. Recently, the atypical B. cereus isolates, which mainly derive from North America and Africa, have drawn great attention due to the potential risk of zoonosis. The cluster of B. cereus carries several anthrax-like virulent genes that are implicated in lethal disease. However, in non-mammals, the distribution of atypical B. cereus is still unknown. In this study, we conducted a retrospective screening of the 32 isolates of Bacillus spp. from diseased Chinese soft-shelled turtles from 2016 to 2020. To recognize the causative agent, we used various methods, such as sequencing analysis using PCR-amplification of the 16S rRNA gene, multiplex PCR for discriminating, and colony morphology by following previous studies. Furthermore, the digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were calculated, respectively, below the 70 and 96% cutoff to define species boundaries. According to the summarized results, the pathogen is taxonomically classified as Bacillus tropicus str. JMT (previous atypical Bacillus cereus). Subsequently, analyses such as targeting the unique genes using PCR and visual observation of the bacteria under various staining techniques were implemented in our study. Our findings show that all (32/32, 100%) isolates in this retrospective screening share similar phenotypical properties and carry the protective antigen (PA), edema factor (EF), hyaluronic acid (HA), and exopolysaccharide (Bps) genes on their plasmids. In this study, the results indicate that the geographic distribution and host range of B. tropicus were previously underestimated.

show abstract

“…But, infectious diseases caused by bacteria and virus have caused severe losses to turtle culture industry (Lu and Jin, 1996;Chen et al, 1999;Hsieh et al, 2006;Zhao et al, 2007). In addition, Chai and Chen (1990a,b) also reported that six species of haemogregarine and two species of trypanosome parasited in soft-shelled turtles.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila

Zhou

Guo

Dai

2008

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Pathological and PCR detection of mycobacteriosis in pond-cultured Chinese soft shell turtles, Trionyx sinensis

Cited by 8 publications

References 17 publications

Mycobacterium ulcerans Infection in an Indian Flap-Shelled Turtle (Lissemys punctata punctata)

Mycobacterium ulcerans Infection in an Indian Flap-Shelled Turtle (Lissemys punctata punctata)

Retrospective Screening of Anthrax-like Disease Induced by Bacillus tropicus str. JMT from Chinese Soft-Shell Turtles in Taiwan

Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila

Contact Info

Product

Resources

About