Pathological changes in the cellular structures of retina and choroidea in the early stages of alloxan-induced diabetes

Abstract

AIMTo investigate the temporal sequence of pathological changes in the cellular structures of retina and choroidea in the early stages of diabetes in laboratory animals.METHODSExperimental type 1 diabetes was modeled by three intraperitoneal injections of an alloxan solution into 30 male nonlinear rats at 16 wk of age. The 30th and 60th days from the final alloxan injection were chosen as the endpoints. Light and electron microscopy and morphometric and immunohistochemical studies were performed on histological slices of eyeballs from experimental animals.RESULTSDiabetic disturbances progressed to 60 d of the experiment. Thus, in the retina, a partial destruction of photoreceptors accompanied by interstitial edema was observed. The morphometric analysis revealed a reduction in the thickness of the retina. A reduction in the number of blood vessels of the choroid with disturbances of the endothelial cells and the vascular walls and a persistent reduction in the number of melanocytes were observed. The number of proliferating Ki-67 positive cells decreased, and the number of macrophages increased with diabetes development.CONCLUSIONThe starting point in the development of destructive changes involves early reduction in the number of melanocytes of the choroidea and alterations in the retinal pigment epithelium.

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Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

“…Unless otherwise noted, all chemicals and reagents were purchased from Sigma-Aldrich (St Louis, MO, United States). T1D was induced by three intraperitoneal injections (100 mg•kg −1 , total dose 300 mg•kg −1 ) of freshly prepared alloxan solution at one-day intervals according to a modified rat model of T1D [21,22]. A day before euthanasia, tail blood was collected for plasma glucose measurements to confirm T1D.…”