Pathological Characterization and Molecular Analysis of <i>Elsinoe</i> Isolates Causing Scab Diseases of Citrus in Jeju Island in Korea

Hyun, Jae-Wook; Timmer, L. W.; Lee, S.-C.; Yun, Su Hyun; Ko, Sang Wook; Kim, K.-S.

doi:10.1094/pdis.2001.85.9.1013

Cited by 41 publications

(40 citation statements)

References 11 publications

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“…1D) (Chung, 2011;Gopal et al, 2014). Because colonies of E. fawcettii and E. australis do not sporulate in artificial media including PDA, Whiteside's method has been widely used for preparing conidia inoculum in vitro (Hyun et al, 2001;Whiteside, 1975). However, sufficient amount of conidia production for study is still a challenge, especially for some isolates or pathotypes.…”

mentioning

confidence: 99%

Improved Method to Increase Conidia Production from Isolates of Different Pathotypes of Citrus Scab Pathogen Elsinoe spp.

Hyun

Paudyal

Hwang

2015

Research in Plant Disease

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Elsinoe fawcettii and E. australis are two currently recognized scab pathogens of citrus. E. fawcettii has at least six pathotypes while E. australis has at least two pathotypes. Colonies of E. fawcettii and E. australis do not sporulate in artificial media including potato dextrose agar (PDA). Whiteside's method has been widely used for preparing conidial inoculum in vitro. This study was carried out to develop efficient method for conidia production from artificial media. We developed a shaking method which included the following steps: 1) Colony grown on PDA was mashed with a steel spatula; 2) Mycelia fragments were cultured in 50 ml sterilized rain water in a rotary shaker-incubator (180 rpm) at 25ºC for 24 h: 3) The conidia suspension was filtered through two layers of cheesecloth. Average conidia production of all isolates tested using this shaking method was approximately 13.1 times higher than that from Whiteside's method in this study.

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confidence: 99%

Improved Method to Increase Conidia Production from Isolates of Different Pathotypes of Citrus Scab Pathogen Elsinoe spp.

Hyun

Paudyal

Hwang

2015

Research in Plant Disease

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“…& Jenkins cause scab disease of Citrus sp. (Hanlin 1989, Hyun et al 2001, Nelson 2008 and there are many other important pathogens of Elsinoe and its asexual states (Table 3). Elsinoe takoropuku G.S.…”

Section: Myriangialesmentioning

confidence: 99%

“…Genera of this family are mostly plant pathogens that cause scab and sunken spots on many economically important plants, such as citrus, grapes, mango, peppers and legumes, reducing their fruit and vegetable values (Mchau et al 1998, Condé et al 1997, Ellis & Erincik 2008, Hyun et al 2001. Species also infect Protea species, reducing the value of the flowers.…”

Section: Introductionmentioning

confidence: 99%

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A re-assessment of Elsinoaceae (Myriangiales, Dothideomycetes)

Jayawardena¹,

Ariyawansa²,

Singtripop³

et al. 2014

Phytotaxa

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The family Elsinoaceae is a relatively poorly known, but important family within Myriangiales, Dothideomycetes. The genera of this family are mostly plant pathogens and causes disease, such as apple and grape scab. In this paper we revisit the family by examining generic types and analysis of molecular sequence data available in GenBank. Elsinoaceae and Myriangeaceae are morphologically and phylogenetically well-supported families in Myriangiales. In Elsinoaceae, 3 to 10 asci form in locules in light coloured pseudoascostromata, which form typical scab-like blemishes on leaf or fruit surfaces, while Myriangeaceae forms raised, superficial, black ascostromata with single asci in each locule, genera may or may not form scab-like lesions. Elsinoe is the type of the family Elsinoaceae and is characterized by forming scab-like blemishes on leaves with few to numerous bitunicate, fissitunicate, globose asci forming inside each locule with a pseudoascostroma containing fungal and host tissues. Following examination of generic type material, Molleriella is retained in Elsinoaceae as it has characters similar to Elsinoe in forming scab-like lesions with pseudoascostromata containing few to numerous bitunicate asci inside each locule. Beelia, Butleria, Hemimyriangium, Hyalotheles, Micularia, Saccardinula, Stephanatheca and Xenodium are excluded from Elsinoaceae and their relative placement in Dothideomycetes is discussed. Fresh collections of these genera are needed so that molecular sequence data can be obtained and analysed to resolve their placement in families or orders of Dothidoemycetes.

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“…It is generally confined to southern South America and has never been reported in the USA (Timmer, 1974). Hyun et al (2001) recovered another pathotype of E. australis from a single tree of C. natsudaidai in South Korea and termed the South American strains as the Sweet Orange pathotype and the Korean strain as the Natsudaidai pathotype. None of the known pathotypes of sweet orange scab affects leaves.…”

Section: Introductionmentioning

confidence: 99%

Sweet orange scab with a new scab disease "syndrome" of citrus in the USA associated with Elsinoë australis

Kunta

Rascoe

et al. 2013

Trop. plant pathol.

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Elsinoë fawcettii causes citrus scab and E. australis causes sweet orange scab and there are different pathotypes of each species. Citrus scab is widely distributed, whereas sweet orange scab is limited mostly to southern South America. In 2010, E. australis was detected by PCR in Texas for the first time in the USA and subsequently found in commercial areas of Texas. In this study, cultures were obtained in Texas mostly from diseased fruit with symptoms similar to "late-season windscar" and identified as the Natsudaidai pathotype of E. australis by sequencing of the PCR amplification products and sequencing of the translation elongation factor and ITS regions. Inoculations of detached leaves and fruit of grapefruit, sweet orange, and tangerine produced scab-like symptoms on all organs and species. The fungus was re-isolated from the inoculated organs and PCR tests on re-isolated fungal colonies and lesions confirmed the presence of E. australis. The disease represents sweet orange scab of citrus with a new scab disease symptomatology, but the host range, ecology and epidemiology of this pathotype are still not completely understood. Thus far, Natsudaidai pathotype has been reported only from South Korea.

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Pathological Characterization and Molecular Analysis of Elsinoe Isolates Causing Scab Diseases of Citrus in Jeju Island in Korea

Cited by 41 publications

References 11 publications

Improved Method to Increase Conidia Production from Isolates of Different Pathotypes of Citrus Scab Pathogen Elsinoe spp.

Improved Method to Increase Conidia Production from Isolates of Different Pathotypes of Citrus Scab Pathogen Elsinoe spp.

A re-assessment of Elsinoaceae (Myriangiales, Dothideomycetes)

Sweet orange scab with a new scab disease "syndrome" of citrus in the USA associated with Elsinoë australis

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