Pathological Features of Healing of a Ruptured Human Round Window Membrane

Sone, Michihiko

doi:10.1159/000027564

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Previous studies using guinea a pig model show that larger holes in RWMs than the holes seen in this study heal spontaneously within a few days without significant damage to hearing (25-29). Our results demonstrated that the designed needle is capable of leaving incisions of an oval shape with the minor axis diameter smaller than the diameter of a 31 gauge needle.…”

Section: Discussionsupporting

confidence: 51%

A dual wedge microneedle for sampling of perilymph solution via round window membrane

Watanabe

Cardoso

Lalwani

et al. 2016

Biomed Microdevices

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Objective Precision medicine for inner-ear disease is hampered by the absence of a methodology to sample inner-ear fluid atraumatically. The round window membrane (RWM) is an attractive portal for accessing cochlear fluids as it heals spontaneously. In this study, we report on the development of a microneedle for perilymph sampling that minimizes size of RWM perforation, facilitates quick aspiration, and provides precise volume control. Methods Considering the mechanical anisotropy of the RWM and hydrodynamics through a microneedle, a 31G stainless steel pipe was machined into wedge-shaped design via electrical discharge machining. Guinea pig RWM was penetrated in vitro, and 1 μ1 of perilymph was sampled and analyzed via UV-vis spectroscopy. Results The prototype wedge shaped needle created oval perforation with minor and major diameter of 143 and 344 μm (n=6). The sampling duration and standard deviation of aspirated volume were seconds and 6.8% respectively. The protein concentration was 1.74 mg/mL. Conclusion The prototype needle facilitated precise perforation of RWMs and rapid aspiration of cochlear fluid with precise volume control. The needle design is promising and requires testing in human cadaveric temporal bone and further optimization to become clinically viable.

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Section: Discussionsupporting

confidence: 51%

A dual wedge microneedle for sampling of perilymph solution via round window membrane

Watanabe

Cardoso

Lalwani

et al. 2016

Biomed Microdevices

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show abstract

“…In middle‐ear structures, elastin contributes to normal development and function in the tympanic membrane [Yan et al, 1988; Ruah et al, 1992], the Eustachian tube [Matsune et al, 1992; Sando et al, 1994], and tendons supporting the ossicular chain [e.g., stapedius tendon; Franz et al, 1993]. The middle layer of the round window membrane contains elastin [Schachern et al, 1982; Sone, 1998], and this layer is sometimes in physical contact with the perilymphatic space of the inner ear [Goycoolea and Lundman, 1997].…”

Section: Discussionmentioning

confidence: 99%

Auditory function and hearing loss in children and adults with Williams syndrome: Cochlear impairment in individuals with otherwise normal hearing

Marler

Sitcovsky

Mervis

et al. 2010

American J of Med Genetics Pt C

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Hearing loss is common in school-age individuals with Williams syndrome (WS) and extensive in adults. Prior studies with relatively small sample sizes suggest that hearing loss in WS has an early onset and may be progressive, yet the auditory phenotype and the scope of the hearing loss have not been adequately characterized. We used standard audiometric tools: Otoscopy, tympanometry, air-conduction (bone conduction when available) behavioral testing, and distortion product otoacoustic emissions (DPOAEs) to measure hearing sensitivity and outer hair cell function. We tested 81 individuals with WS aged 5.33 to 59.50 years. 63% of the school-age and 92% of the adult participants had mild to moderately-severe hearing loss. The hearing loss in at least 50% was sensorineural. DPOAE testing corroborated behavioral results. Strikingly, 12 of 14 participants with hearing within normal limits bilaterally had 4000-Hz DPOAE input/output (DPOAE IO) functions indicative of outer hair cell damage and impaired cochlear compression. Our results indicate that hearing loss is very common in WS. Furthermore, individuals with WS who have “normal” hearing as defined by behavioral thresholds may actually have sub-clinical impairments or undetected cochlear pathology. Our findings suggest outer hair cell dysfunction in otherwise normal hearing individuals. The DPOAE IO in this same group revealed growth functions typically seen in groups with noise-induced damage. Given this pattern of findings, individuals with WS may be at increased risk of noise-induced hearing loss. Recommendations regarding audiological testing for individuals with WS and accommodations for these individuals in both academic and nonacademic settings are provided.

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Pathological Features of Healing of a Ruptured Human Round Window Membrane

Cited by 2 publications

References 12 publications

A dual wedge microneedle for sampling of perilymph solution via round window membrane

A dual wedge microneedle for sampling of perilymph solution via round window membrane

Auditory function and hearing loss in children and adults with Williams syndrome: Cochlear impairment in individuals with otherwise normal hearing

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