Pathological investigation of Marek’s disease in a pullet farm of Bangladesh and detection of Marek’s disease virus by pcr

The present investigation was carried out on layer flock belong to Livestock farm complex, College of Veterinary Science, Tirupati. Among 500 birds 41 birds revealed lesions suspected for Marek's disease and morphologically diagnosed on the basis of gross lesions, cytological smears and immunocytochemistry of different organs. Haematological and biochemical examination revealed significant increase in the lymphocytes count, AST, triglyceride levels and significant decrease in cholesterol levels in the Marek's disease affected birds when compared to normal birds. Clinically, birds affected with Marek's disease revealed emaciation, depression with drooping of the wings, anorexia, gasping, reduced weight gain, diarrhoea, pale combs, wattles with presence of nodules on combs, marked reduction in egg production and reduction in size of eggs. Few birds exhibited paralysis with inward curvature of the toes and death of the birds in few days after showing the clinical signs. Grossly, visceral organs (liver, heart, lungs, kidney, ovary, spleen, thymus, pancreas, proventriculus) were enlarged with presence of focal to discrete nodules. Cytological examination of the impression smears collected from the growths of the affected organs during necropsy revealed infiltration of pleomorphic lymphoid cells with thin rim of basophilic cytoplasm. Immunocytochemical staining of the smears revealed moderate to severe immunopositive reactivity for MDV antigens in the cytoplasm of the lymphoid cells.

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“…(1971) [6] , Santin et al (2006) [19] , Keles et al (2010) [11] , Sawale et al (2014) [20] , Biswas et al (2018) [4] and Das et al…”

Section: Biochemical Parametersmentioning

confidence: 99%

Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

Reddy¹,

Kumar²,

Amaravathi³

et al. 2021

Pharma Innovation

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The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al‐Najaf, Iraq

Al-Zebeeby,

Abbas,

Alsaegh

et al. 2024

Veterinary Medicine International

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Marek’s disease (MD) is a highly infectious poultry illness with a tendency to form tumours in peripheral nerves and internal organs of affected birds. Tumours accompany MD, mostly caused by oncogenic Gallid alpha herpesvirus 2 (MD Herpes virus serotype I). Studies on avian tumours associated with MD infection are limited in Iraq. In the presented study, the positive samples of ocular tumour were 168 out of 282 MD positive samples, which accomplished in farm suffered from an unexpectedly high mortality rate. We investigated a rapidly developed tumour mass that was observed in an MD‐vaccinated layer flock that showed obvious clinical signs of MD, accompanied by forming a small lump in one eye at age 21 weeks, which developed to a big lump at week 28 of age, leading to death. The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. Histopathological examination of the ocular tumour core revealed aggregation of neoplastic cells and haemorrhage that replaced the normal eye tissue, as well as early tumour formation in internal organs such as the lung and liver. In addition, abnormal lesions are susceptible to tumours in the gizzard and spleen. To our knowledge, this is the first record of an aggressive MD virus infection‐mediated ocular tumour in a layer flock in Al‐Najaf province, Iraq.

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Pathological investigation of Marek’s disease in a pullet farm of Bangladesh and detection of Marek’s disease virus by pcr

Cited by 2 publications

References 21 publications

Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al‐Najaf, Iraq

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