Pathological Mitosis and Mixoploidy in the Meristematic Tissues of Grape Plant

Klimenko, V. P.

doi:10.1134/s1062360419020024

Cited by 1 publication

(2 citation statements)

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“…However, somatic segregation leads to the appearance of cells with an unequal number of chromosomes in the tissues. This phenomenon can be caused by various reasons, including endomitosis [14]. Mixoploidy was also observed in the cells of experimental in vitro grape plants obtained from stenospermocarpic seeds, as well as in the meristematic tissue of the mutant grape variety Aurora Magaracha [14,17].…”

Section: Resultsmentioning

confidence: 99%

“…Grape somaclones were obtained according to the protocols developed at the Magarach Institute (treatment of proembryogenic cells with colchicine, development of somatic embryoids from colchicinated cells of suspension cultures and regeneration of somaclone plants from them) [13]. Cytogenetic analysis was performed according to the methods recommended for grapes [14]. Roots 3-5 mm long of in vitro plants were used as objects, the meristem of root growth points was studied.…”

Section: Methodsmentioning

confidence: 99%

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Polyploidy in tissues of plants in vitro of grape somaclones

2021

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In vitro experimental plants obtained by clonal micropropagation of 9 grape somaclones of 5 original forms were the material for cytogenetic research. A biological microscope XSP-146TP was used for cytogenetic analysis. 823 cases of deviation from diploidy were observed in total. Significant tissue ploidy was observed in the meristematic tissue of in vitro plants of grape somaclones obtained by colchicine treatment of proembryogenic cells of various varieties. The significant direct correlation was found between the frequency of polyploidy in meristem tissues of in vitro plants and the number of chloroplasts in the stomata of grape somaclones. The reverse correlation was found between the frequency of polyploidy and the number of stomata on the leaf area. Somaclone No. 72, obtained as a result of regeneration from colchicinated proembryogenic cells of the Ruta grape variety and identified as a tetraploid (2n = 4x = 76), is recommended for use in the polyploid creation program.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%