Pathological observations of an experimental infection of geese with goose circovirus

Guo, Jing; Tian, Jing; Tan, Xiaohong; Huang, Yu; Ding, Shou-Qiang; Sun, Huiyong; Yu, Xiaohong

doi:10.1080/03079457.2010.538371

Cited by 26 publications

(25 citation statements)

References 29 publications

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“…The circovirus infection of Anseriformes is multi-systemic and the virus can be detected in the bursa of Fabricius, spleen, liver, kidney and thymus [ 18 , 10 ]. The bursa is the most predominant place for circovirus replication, in birds.…”

Section: Discussionmentioning

confidence: 99%

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A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus

Matczuk

Krawiec

Wieliczko

2015

Virol J

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BackgroundThe aim of this study was to investigate the presence of circoviruses in wild bird populations, in Poland. Circoviruses possess immuno-suppressive properties and might interfere with the health of wild birds.Method83 birds, which belonged to 23 species, were tested with broad-range, nested PCR. The obtained PCR products were sequenced and new primers designed, to analyse the full-length, viral genome. A phylogenetic analysis was conducted, to find any relationship to known circoviruses.ResultsThe circovirus DNA sequence was found in 4 birds. All samples originated from the velvet scoter (Melanitta fusca) a marine duck from the Merginae sub-family. Birds which tested positive for the circovirus were found dead in fishing nets, off the Baltic coast. During post-mortem examination, carcasses of two of the scoters showed only light emaciation, while the two other birds appeared healthy. The obtained, full-length, circovirus sequence revealed 1,988 nucleotides and the presence of typical features (i.e. Cap, Rep and ORF3). Nucleotide similarity to other duck circoviruses was 84 to 86 %. Phylogenetic analysis of the complete genome and cap gene, indicated that the new circovirus is related to known duck circoviruses, especially to sub-types sometimes referred to as duck circovirus genotype 1, but not genotype 2.ConclusionsIn this study, we have reported a new duck circovirus sequence detected in the velvet scoter, a species of marine duck. Sequence comparison and phylogenetic analysis of the new virus sequence support previous reports that duck circovirus (DuCV) is a species with a high degree of diversity. The viral sequence obtained from the velvet scoter suggests that DuCV may infect birds from the Anatinae sub-family. More studies are needed to prove if the velvet scoter and other marine ducks act as a reservoir for DuCV.

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Section: Discussionmentioning

confidence: 99%

“…However, based on the genomic sequences of DuCV, some scientists divide this circovirus into two genotypes; DuCV 1 and DuCV 2 [ 8 , 9 ]. A similar disease occurs in domestic geese and is caused by the closely related goose circovirus (GoCV) [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus

Matczuk

Krawiec

Wieliczko

2015

Virol J

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“…An experimental approach for understanding the route of infection and pathology of the infection with circoviruses has recently been studied by Guo et al . [14] which confirmed and important role of these viruses in the clinical form of the disease manifested as severe feather disorders. However diagnosis drawn only on the basis of clinical symptoms themselves should also be confirmed by other techniques including histological assays, electron microscopy, serological assays, in situ hybridization [28] or different variants of polymerase chain reaction (PCR) [19].…”

Section: Discussionmentioning

confidence: 68%

“…Geese affected with GCV may show growth retardation or feathering disorders. The most common histopathological changes are observed as depletion of T-lymphocytes and histocystis in lymphoid organs including thymus, spleen and bursa of Fabricius (BF) [14]. Diagnosis of GCV may help in isolation of the affected birds from the rest of the flock thus minimizing huge loss due to the GCV immunosuppressive influence.…”

Section: Introductionmentioning

confidence: 99%

“…So far the presented reports described isolation of GoCV in cell cultures that is laborious and needs SPF goose embryos, histology and electron microscopy which on the other hand require operation by an experienced pathologist [14]. The polymerase chain reaction (PCR) era of amplification of multiple genes and cloning of the whole DNA viral genomes facilitated the development of novel and sensitive techniques like nested-PCR and real-time PCR which allowed for firm and fast diagnosis as well as sequencing of circoviral genomes [13,15-19].…”

Section: Introductionmentioning

confidence: 99%

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Loop-mediated isothermal amplification for the detection of goose circovirus

Woźniakowski

Kozdrun

Samorek-Salamonowicz

2012

Virol J

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BackgroundGoose circovirus (GCV) presents an immunosuppressive problem in production of geese. The infection’s clinical symptoms include growth retardation or feathering disorders but the infection process may remain non-symptomatic what makes the infected birds more susceptible for secondary viral, bacterial and fungal infections. Diagnosis of GCV infection is made by histopathological examination, dot blot hybridization, polymerase chain reaction (PCR) and real-time PCR. However these techniques require application of thermocyclers and qualified staff which may be cost-consuming for some diagnostic units. The aim of this study was to develop loop-mediated isothermal amplification assay (LAMP) as a simple method of GCV detection.ResultsThe presented study has shown LAMP as a rapid tool of detecting DNA of goose circovirus (GCV) as soon in 30 min time. The method used three sets of primers: two outer primers (F3 and B3), two inner primers (FIP and BIP) and two loop primers (FL and BL) to accelerate the reaction. The optimum reaction temperature and the time were 61°C for 30 min, respectively. The results were analysed using SYBR Green dye and GelRedTM solutions. Thirty-eight isolates of GCV collected from geese flocks in Poland were examined. For comparison, real-time polymerase chain reaction with F3 and B3 primers and SYBR Green dye was conducted. The obtained results have shown GCV-LAMP as a sensitive, rapid and specific assay and alternative for PCR-based methods.ConclusionsThe developed technique due to its simplicity may be applied by any veterinary laboratory or even mobile diagnostics units for the routine detection of GCV.

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Integument

2015

Pathology of Pet and Aviary Birds

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Pathological observations of an experimental infection of geese with goose circovirus

Cited by 26 publications

References 29 publications

A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus

A new duck circovirus sequence, detected in velvet scoter (Melanitta fusca) supports great diversity among this species of virus

Loop-mediated isothermal amplification for the detection of goose circovirus

Integument

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