Pathological Study of Oral Squamous Cell Carcinoma by Application of P53 and PCNA (Immunohistochemical Approach)

Abdulkadir, Susan N.; Ali, Abbas R.; Alchalabi, Najwa J.

doi:10.20546/ijcmas.2016.504.013

Cited by 5 publications

(3 citation statements)

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“…Gupta et al 48 suggested that the expression pattern of PCNA could be used to determine the extent of neoplastic transformation as well as to assess the histological grades of oral malignancy. Abdulkadir et al 49 reported that PCNA immunoexpression pattern could be used as a prognostic indicator in all the histological grades of oral malignancy. Lončarevićet al 50 correlated the PCNA expression with the degree of peritumoral tissue infiltration.…”

Section: Resultsmentioning

confidence: 99%

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

2020

TJNPR

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Abnormal cell proliferation, invasion, metastasis and angiogenesis are the most prominent features of malignant tumours. The present study evaluated the modulating effect of myrtenal on the immunoexpression pattern of cell proliferative (PCNA and cyclin D1), angiogenic (VEGF) and invasive (MMP-2 and MMP-9) markers in 7,12-dimethylbenz(a)anthracene (DMBA)induced experimental oral carcinogenesis in golden Syrian hamsters using immunohistochemical assay. Topical application (painting) of 0.5% DMBA (six hamsters), a site specific carcinogen, three times a week for 14 weeks resulted in the formation of tumors in the buccal pouches of golden Syrian hamsters , which was confirmed by histopathological studies. Buccal mucosa excised from the hamsters treated with DMBA alone (tumour-bearing hamsters) showed abnormal immunoexpression pattern of cell proliferative, angiogenic and invasive markers. Myrtenal administration (230 mg/kg b.w) orally to DMBA treated hamsters significantly downregulated the expression of the above said molecular markers in the chemopreventive phase and considerably decreased the expression pattern in the chemotherapeutic phase. The findings from this study, thus support the anti-cell proliferative, anti-angiogenic, and antiinvasive potential of myrtenal in DMBA-induced hamster buccal pouch carcinoma.

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Section: Resultsmentioning

confidence: 99%

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

2020

TJNPR

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“…Abdulkadir et al (2010) from their study suggested that the expression of PCNA was a good indicator of the aggressiveness of the lesion [16]. Madan et al (2015) showed that PCNA was a good marker in differentiating normal epithelium from oral squamous cell carcinoma [9].…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Cluster of Differentiation 57 and Proliferating Cell Nuclear Antigen Expression in Different Grades of Oral Squamous Cell Carcinoma: An Immunohistochemical Study

CS,

Joseph,

Girish

et al. 2023

Cureus

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Background: The immune defense against tumor cells is mainly mediated by the natural killer (NK) cells. Cluster of differentiation 57 (CD57) is a 110-kd glycoprotein, typically expressed by the NK cells, attacks the cancer cells and inhibits tumor development. Proliferating cell nuclear antigen (PCNA) is a 36-kd auxiliary protein for DNA polymerase delta that correlates with cell proliferation and DNA synthesis. It is an essential component of DNA replication, DNA recombination, and DNA repair. The uncoordinated proliferation of PCNA protein characterizes the biological behavior of malignant lesions. Aim: The aim of the present study is to compare and correlate the expression of CD57 and PCNA in different grades of oral squamous cell carcinoma (OSCC) by immunohistochemistry. Materials and methods: This retrospective analysis comprises 30 samples of various grades of OSCCs and 10 samples of healthy mucosa. Sections of 4-5 µm thickness were done and stained with monoclonal anti-PCNA and anti-CD57 antibodies. The statistical package for social science (SPSS) version 16.0 software (IBM Corp., Armonk, NY) was used to analyze the data in this study. The expression of CD57 and PCNA was compared and correlated between the groups using analysis of variance (ANOVA) post hoc, Dunnet t-test , and Pearson's correlation coefficient test. For statistical significance, a p-value of 0.05 or less was used. Results: A significant decrease in CD57 labeling index was seen from well-differentiated squamous cell carcinoma (16.63 ± 2.33) to poorly differentiated squamous cell carcinoma (5.53 ± 1.20) whereas the significant increase in PCNA labeling index was noted from well-differentiated squamous cell carcinoma (45.88 ± 2.20), followed by moderately differentiated and poorly differentiated squamous cell carcinoma (72.77 ± 4.35). Conclusion: The combination of CD57 and PCNA biomarkers appears to be good indicators of the immune status of the patient and the aggressiveness of the lesion.

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“…5 The development of cancer is a complex and multi-step process which includes degradation of the basement membrane and extracellular matrix, loss of cell adhesiveness, tumour cell motility and formation of new blood vessels. [6][7] Although PEH shows no cytological evidence of malignancy microscopically, still it can be mistaken for an invasive OSCC. 8 In essence it is significantly important to differentiate between the two pathologies as the therapeutic management is entirely different.…”

Section: Introduction Introductionmentioning

confidence: 99%

Expression of Matrix Metalloproteinase-9 in Oral Squamous Cell Carcinoma and Oral Pseudoepitheliomatous Hyperplasia

Awan

Naz

Mahmood

et al. 2020

GJMS

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Background: Oral pseudoepitheliomatous hyperplasia (PEH) appears histologically similar to oral squamous cell carcinoma (OSCC) in small oral biopsies, thus posing diagnostic dilemma. The objective of this study was to compare the expression of matrix metalloproteinase-9 (MMP-9) in differential diagnosis of OSSC and oral PEH. Materials & Methods: This comparative cross-sectional study was conducted in the Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan from January 2013 to March 2016. It included sixty archival cases, 30 each of OSCC and PEH. Paraffin embedded blocks were prepared, hematoxylin and eosin stained sections taken and immunostained with MMP-9. The expression of MMP-9 was evaluated in OSCC and PEH. Results: The OSCC group included 16 (53.33%) men and 14 (46.67%) women, whereas PEH group included 18 (60%) men and 12 (40%) women. The mean age of OSCC group was 60.1±17.3 and that of PEH group was 52.7±16.6. In OSCC group, site of lesion was buccal mucosa in 12 (40%), gingiva 10 (33.33%), tongue 7 (23.33%) and floor of mouth 1 (3.34%) case. In PEH group, site of lesion was buccal mucosa in 12 (40%), tongue 11 (36.66%), gingiva 6 (20%) and palate 1 (3.34%) case. The expression of MMP-9 was positive in all the 30 cases of OSCC and negative in all 30 cases of PEH. Conclusion: Compared to pseudoepitheliomatous hyperplasia (PEH), MMP-9 revealed a higher expression in oral squamous cell carcinoma (OSCC). This finding has become mainstream strategy in distinguishing OSCC from PEH in oral mucosal biopsies in cases difficult to diagnose.

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Pathological Study of Oral Squamous Cell Carcinoma by Application of P53 and PCNA (Immunohistochemical Approach)

Cited by 5 publications

References 0 publications

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

Comparative Analysis of Cluster of Differentiation 57 and Proliferating Cell Nuclear Antigen Expression in Different Grades of Oral Squamous Cell Carcinoma: An Immunohistochemical Study

Expression of Matrix Metalloproteinase-9 in Oral Squamous Cell Carcinoma and Oral Pseudoepitheliomatous Hyperplasia

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