Pathology and Biofilm Formation in a Porcine Model of Staphylococcal Osteomyelitis

Johansen, Louise Kruse; Koch, Julian; Frees, Dorte; Aalbæk, Bent; Nielsen, Ole Lerberg; Leifsson, Páll S.; Iburg, Tine; Svalastoga, E.; Buelund, Lene E.; Bjarnsholt, Thomas; Høiby, Niels; Jensen, Henrik Elvang

doi:10.1016/j.jcpa.2012.01.018

Cited by 29 publications

(78 citation statements)

References 33 publications

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“…Although hematogenous osteomyelitis was successfully established in all three intra-arterial inoculation studies [3,7,9], there were problems with cellulitis [3] and sepsis [9]. However, in the last reported porcine intra-arterial inoculation model [7] there were no complications due to the technique per se. In that model the inoculation technique was based on bacterial injection into the femoral artery using the retrograde modified Seldinger technique (non-percutaneous) [10].…”

Section: Introductionmentioning

confidence: 99%

“…Staphylococcal osteomyelitis occurs naturally in humans and pigs [1,2], and lesions can be reproduced experimentally in porcine models [3][4][5][6][7]. The general similarities between human and porcine physiology and pathophysiology make porcine models of osteomyelitis advantageous [8].…”

Section: Introductionmentioning

confidence: 99%

“…The general similarities between human and porcine physiology and pathophysiology make porcine models of osteomyelitis advantageous [8]. A variety of inoculation routes have been used in previously described porcine models of osteomyelitis, i.e., traumatic [5,6], intravenous [4], and intra-arterial [3,7]. Because of the localized and predictive nature of infection, inoculation in the arterial supply to selected bones should be the preferred method for establishing hematogenous osteomyelitis.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the localized and predictive nature of infection, inoculation in the arterial supply to selected bones should be the preferred method for establishing hematogenous osteomyelitis. Apart from the two porcine studies on intra-arterial inoculation [3,7], only this route has been previously used for development of hematogenous osteomyelitis in a canine model [9]. Although hematogenous osteomyelitis was successfully established in all three intra-arterial inoculation studies [3,7,9], there were problems with cellulitis [3] and sepsis [9].…”

Section: Introductionmentioning

confidence: 99%

“…Apart from the two porcine studies on intra-arterial inoculation [3,7], only this route has been previously used for development of hematogenous osteomyelitis in a canine model [9]. Although hematogenous osteomyelitis was successfully established in all three intra-arterial inoculation studies [3,7,9], there were problems with cellulitis [3] and sepsis [9]. However, in the last reported porcine intra-arterial inoculation model [7] there were no complications due to the technique per se.…”

Section: Introductionmentioning

confidence: 99%

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A New Technique for Modeling of Hematogenous Osteomyelitis in Pigs: Inoculation into Femoral Artery

Johansen

Svalastoga

Frees³

et al. 2012

Journal of Investigative Surgery

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A new inoculation technique has been developed and applied in a porcine model of juvenile hematogenous osteomyelitis. Following the success of the model, we describe the inoculation technique in detail to enable its replication in future studies. The technique was based on an anatomical feature of the femoral artery that enables inoculation into the artery using a simple surgical procedure. Inoculation in the femoral artery is advantageous because the localization of lesions constitutes a discriminative model of the naturally occurring hematogenous osteomyelitis in long bones, usually involving femur and tibia in children. The procedure was performed under general anesthesia and consisted of five major steps: (1) Exposure of the right femoral artery, (2) retrograde catheterization, (3) inoculation of bacteria, (4) hemostasis of the arterial puncture site using compression, and (5) suturing of the wound in two layers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A New Technique for Modeling of Hematogenous Osteomyelitis in Pigs: Inoculation into Femoral Artery

Johansen

Svalastoga

Frees³

et al. 2012

Journal of Investigative Surgery

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Innovations in osteomyelitis research: A review of animal models

Roux

Cobb

Seitz

et al. 2021

Anim Models and Exp Med

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Infection of bone tissue, or osteomyelitis, has become a growing concern in modern healthcare due in no small part to a rise in antibiotic resistance among bacteria, notably Staphylococcus aureus. The current standard of care involves aggressive, prolonged antibiotic therapy combined with surgical debridement of infected tissues. While this treatment may be sufficient for resolving a portion of cases, recurrences of the infection and associated risks including toxicity with long‐term antibiotic usage have been reported. Therefore, there exists a need to produce safer, more efficacious options of treatment for osteomyelitis. In order to test treatment regimens, animal models that closely mimic the clinical condition and allow for accurate evaluation of therapeutics are necessary. Establishing a model that replicates features of osteomyelitis in humans continues to be a challenge to scientists, as there are many variables involved, including choosing an appropriate species and method to establish infection. This review addresses the refinement of animal models of osteomyelitis to reflect the clinical disease and test prospective therapeutics. The aim of this review is to explore studies regarding the use of animals for osteomyelitis therapeutics research and encourage further development of such animal models for the translation of results from the animal experiment to human medicine.

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Exopolysaccharide Production and Biofilm Formation by Histophilus somni

Petruzzi

Inzana

2015

Current Topics in Microbiology and Immunology

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The biofilm matrix of Histophilus somni is a complex architecture that differs substantially in structure between a pathogenic and commensal isolate examined. Overall, most pathogenic isolates produce more biofilm than commensal isolates. A major component of the biofilm is exopolysaccharide (EPS), which is also produced in greater quantity in the pathogenic isolate than in the commensal isolate studied. The EPS is composed of a D-mannan polymer, with occasional galactose residues present on side chains, similar in composition to that of yeast mannan. When grown in the presence of sialic acid, the biofilm EPS becomes sialylated and the amino sugars N-acetylglucosamine and N-acetylgalactosamine can be detected. In vitro biofilm formation follows a typical 4-stage growth curve, characterized by attachment, growth, maturation, and detachment. Following experimental challenge, formation of an H. somni biofilm has been demonstrated in cardiopulmonary tissue, often with Pasteurella multocida cohabitating the biofilm. A recently developed diagnostic test can detect antibodies to the EPS only in animals with systemic disease due to H. somni and is therefore capable of distinguishing between healthy animals colonized with H. somni and animals with systemic disease.

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Pathology and Biofilm Formation in a Porcine Model of Staphylococcal Osteomyelitis

Cited by 29 publications

References 33 publications

A New Technique for Modeling of Hematogenous Osteomyelitis in Pigs: Inoculation into Femoral Artery

A New Technique for Modeling of Hematogenous Osteomyelitis in Pigs: Inoculation into Femoral Artery

Innovations in osteomyelitis research: A review of animal models

Exopolysaccharide Production and Biofilm Formation by Histophilus somni

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