Pathomorphological and Immunohistological Findings in Progeny of Goats Experimentally Infected with Pestiviruses

Wohlsein, P.; Trautwein, G.; Depner, Klaus; Hübschle, O. J. B.; Ließ, B.

doi:10.1111/j.1439-0450.1992.tb01131.x

Cited by 5 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have demonstrated that pregnant goats will experience widespread abortions after inoculation with BVDV, and abortions, stillbirths, and persistently infected lambs result from experimental inoculation of pregnant sheep. 4, 15, 20, 21 A recent report documented the establishment of a PI white-tailed deer after intranasal inoculation of BVDV-1 and BVDV-2. 18 Although the inoculation and subsequent fetal lesions of these animals demonstrate the pathogenicity of BVDV to the goat, sheep, and deer fetus, it had been previously unclear if natural transmission of the virus from cattle to goats can result in similar fetal lesions.…”

Section: Discussionmentioning

confidence: 99%

Bovine Viral Diarrhea Virus Abortion in Goats Housed with Persistently Infected Cattle

et al. 2009

View full text Add to dashboard Cite

Twenty-four border disease virus-seronegative, pregnant, mixed breed goats were experimentally comingled with 3 heifers persistently infected with bovine viral diarrhea virus type 2a (BVDV-2a). Twelve of the 24 exposed does aborted. Twenty-nine fetuses and 16 placentas from affected does were submitted to the Oklahoma Animal Disease Diagnostic Laboratory for a necropsy examination. Infection with BVDV was confirmed with a combination of immunohistochemistry, BVDV-2 polymerase chain reaction, and virus isolation in 19 of the 29 fetuses. On gross examination of the 19 fetuses and placentas in which BVDV-2a infection was confirmed, a mild placentitis (3/19), fetal mummification (1/19), and facial deformities (4/19) were noted. Histologically, placentitis (2/19), myocarditis (4/19), thymic depletion (5/19), choroid plexitis (3/19), encephalitis (2/19), and cerebral gliosis (1/19) were noted. Other causes of abortion in goats, including common bacterial and viral infections, were ruled out with histology, virus isolation, polymerase chain reaction, and aerobic bacteriologic cultures. As supported by the findings in this case, BVDV-2a should be included as a differential for abortion in goats. This is the first report of abortion in goats after exposure to persistently infected cattle.

show abstract

Section: Discussionmentioning

confidence: 99%

Bovine Viral Diarrhea Virus Abortion in Goats Housed with Persistently Infected Cattle

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Brain, intestine, kidney, liver, lung, placenta, spleen, thymus Scherer et al 17 Heart, intestine, placenta, skin Swasdipan et al 18 Caprine Brain Wohlsein et al 20 No positive staining Baszler et al…”

mentioning

confidence: 99%

“…3 In previous studies, BVDV antigen has been detected with immunohistochemistry (IHC) in the brain of aborted goat fetuses but was not demonstrated in other organs. 1,20 The present study will demonstrate the distribution of BVDV antigen in infected goat fetuses using IHC.…”

mentioning

confidence: 99%

Distribution of Bovine Viral Diarrhea Virus Antigen in Aborted Fetal and Neonatal Goats by Immunohistochemistry

2009

View full text Add to dashboard Cite

Abstract. Bovine viral diarrhea virus (BVDV) infection in goats can result in severe reproductive losses, with abortion rates reaching 80%. Infection with BVDV in aborted goat fetuses and stillborn kids can result in placentitis, encephalitis, myocarditis, and thymic depletion. This study investigates the distribution of viral antigen within the organ systems of aborted goat fetuses, stillborn kids, and nonviable kids infected with BVDV at various stages of gestation using immunohistochemistry (IHC). Virus antigen was detected within the placenta (8/13), thymus (4/9), heart (4/11), and brain (4/15) of affected goats. Uncommonly, BVDV antigen was detected within the skin (1/14), liver (1/13), kidney (1/12), lung (1/11), and trachea (1/3). BVDV antigen was not detected within the spleen (0/9), nasal turbinate (0/2), or thyroid (0/3). The results of this study indicate that placenta, heart, thymus, and brain are the most reliable tissues for BVDV antigen detection using IHC in aborted goat fetuses.

show abstract

“…13 BDV infections have been reported worldwide and so lead to significant losses in the small ruminant population. 8,10,24,25,28 In October 2004, we encountered newborn twin lambs showing nervous clinical signs and abnormal fleece, with long and straight birth coats, in a sheep flock in Aydin-Kuyucak, Aegean region of Turkey. Pathologic and immunohistochemical examinations indicated pestivirus infection in the lambs.…”

mentioning

confidence: 99%

Neuropathologic Study of Border Disease Virus in Naturally Infected Fetal and Neonatal Small Ruminants and Its Association With Apoptosis

Toplu

Oğuzoğlu²,

Epikmen³

et al. 2010

Vet Pathol

View full text Add to dashboard Cite

The present study describes the pathologic changes and cellular apoptosis in the central nervous system (CNS) of fetal and neonatal small ruminants infected with border disease virus (BDV), as demonstrated by immunohistochemistry and in situ hybridization. Abortions of ewes and goats were observed, as were births of lambs and kids with poor survival rates and nervous signs. Lesions included cerebellar hypoplasia, porencephaly, hydranencephaly, and nonsuppurative meningoencephalomyelitis with hypomyelinogenesis. Viral antigens and RNA were present in neuropil, glial, and neuronal cells, especially in periventricular areas, cerebellum, and brainstem. TUNEL positivity and labeling of anti-bax and anti-caspases 3, 8, and 9 were detected in BDV-infected CNSs, especially in glial and neuronal cells. The double immunostaining and TUNEL assay revealed that in BDV-infected animals, not only were BDV-infected glial and neuronal cells undergoing apoptosis, but so were uninfected cells in close vicinity of BDV-infected cells. The expression of activated caspases 3, 8, 9; bax; and TUNEL in glial and neuronal cells of the infected fetal and neonatal kids were significantly (P < .05) higher than those of the infected fetal and neonatal lambs. Yet, the expression of bcl-2 in the CNSs of the infected fetal and neonatal lambs was higher (P < .05) in neuronal and glial cells than in those of the infected fetal and neonatal kids. The results suggest that cell death in the BDV-infected CNS is induced by intrinsic and extrinsic cascades of apoptotic pathways.

show abstract

Pathomorphological and Immunohistological Findings in Progeny of Goats Experimentally Infected with Pestiviruses

Cited by 5 publications

References 23 publications

Bovine Viral Diarrhea Virus Abortion in Goats Housed with Persistently Infected Cattle

Bovine Viral Diarrhea Virus Abortion in Goats Housed with Persistently Infected Cattle

Distribution of Bovine Viral Diarrhea Virus Antigen in Aborted Fetal and Neonatal Goats by Immunohistochemistry

Neuropathologic Study of Border Disease Virus in Naturally Infected Fetal and Neonatal Small Ruminants and Its Association With Apoptosis

Contact Info

Product

Resources

About