Pathophysiology of chikungunya virus infection associated with fatal outcomes

de Souza, William M.; Fumagalli, Marcilio J.; de Lima, Shirlene T.S.; Parise, Pierina L.; Carvalho, Deyse C.M.; Hernandez, Cristian; de Jesus, Ronaldo; Delafiori, Jeany; Candido, Darlan S.; Carregari, Victor C.; Muraro, Stefanie P.; Souza, Gabriela F.; Simões Mello, Leda M.; Claro, Ingra M.; Díaz, Yamilka; Kato, Rodrigo B.; Trentin, Lucas N.; Costa, Clauber H.S.; Maximo, Ana Carolina B.M.; Cavalcante, Karene F.; Fiuza, Tayna S.; Viana, Vânia A.F.; Melo, Maria Elisabeth L.; Ferraz, Clarissa P.M.; Silva, Débora B.; Duarte, Larissa M.F.; Barbosa, Priscilla P.; Amorim, Mariene R.; Judice, Carla C.; Toledo-Teixeira, Daniel A.; Ramundo, Mariana S.; Aguilar, Patricia V.; Araújo, Emerson L.L.; Costa, Fabio T.M.; Cerqueira-Silva, Thiago; Khouri, Ricardo; Boaventura, Viviane S.; Figueiredo, Luiz Tadeu M.; Fang, Rong; Moreno, Brechla; López-Vergès, Sandra; Mello, Liana Perdigão; Skaf, Munir S.; Catharino, Rodrigo R.; Granja, Fabiana; Martins-de-Souza, Daniel; Plante, Jessica A.; Plante, Kenneth S.; Sabino, Ester C.; Diamond, Michael S.; Eugenin, Eliseo; Proença-Módena, José Luiz; Faria, Nuno R.; Weaver, Scott C.

doi:10.1016/j.chom.2024.02.011

Cited by 8 publications

(1 citation statement)

References 100 publications

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“…Chikungunya is a disease caused by a virus and transmitted by the mosquito Aedes aegypti or Aedes albopictus (Kemenkes RI, 2012). Factors related to the Chikungunya outbreak first the state of the Water Reservoir (TPA) (Badoni et al, 2023;de Souza et al, 2024;Ngwe Tun et al, 2023). In the rainy season, the population of Aedes aegypti will increase because the eggs that had not had time to hatch will hatch when the breeding habitat (landfill is not a daily and natural necessity) begins to fill with rainwater (Kemenkes RI, 2012).…”

Section: Introductionmentioning

confidence: 99%

Factors Related to the Chikungunya Outbreak (Extraordinary Events) in Wonoboyo Sub-District, Wonogiri District, Wonogiri District

Maharani,

Sari

2024

AJESH

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Chikungunya, a viral disease transmitted by Aedes aegypti or Aedes albopictus mosquitoes, poses a significant public health threat. In Wonoboyo Village, according to data from the Wonogiri Health Office, there were 119 reported cases in 2020, and 61 cases in 2021, highlighting the local impact of this disease within Wonogiri Regency. This study endeavors to investigate the factors contributing to the Chikungunya outbreak specifically in Wonoboyo Village. The research adopts an analytical observational approach with a cross-sectional design. The study's variables include the state of the landfill, the presence of larvae, occupancy density, landfill maintenance habits, hygiene practices, and mosquito repellent usage. The dependent variable is the occurrence of the Chikungunya outbreak (KLB Chikungunya). Data collection involves questionnaires and observations among the residents of Wonoboyo Village, with a sample size of 357 households selected through simple random sampling. Analysis utilizing Chi-square tests, with a significance level of 5%, reveals correlations between various factors and the Chikungunya outbreak. Specifically, the condition of the landfill, presence of larvae, occupancy density, landfill maintenance habits, hygiene practices, and mosquito repellent usage are significantly associated with the outbreak. Based on the findings, it is recommended that residents of Wonoboyo Village adhere to PSN 3M activities regularly and maintain cleanliness both indoors and outdoors to mitigate the transmission of Chikungunya.

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Section: Introductionmentioning

confidence: 99%

Factors Related to the Chikungunya Outbreak (Extraordinary Events) in Wonoboyo Sub-District, Wonogiri District, Wonogiri District

Maharani,

Sari

2024

AJESH

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Differential restriction of chikungunya virus in primary human cardiac endothelial cells occurs at multiple steps in the viral life cycle

Spector,

Noval,

Stapleford

2024

Preprint

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Arthropod-borne viruses (arboviruses) constitute a significant ongoing public health threat, as the mechanisms of pathogenesis remain incompletely understood. Cardiovascular symptomatology is emerging as an important manifestation of arboviral infection. We have recently studied the cardiac tropism and mechanisms implicated in cardiac damage in mice for the alphavirus chikungunya virus (CHIKV), and we therefore sought to evaluate the cardiac tropism of other emerging alphaviruses and arboviruses. Using human primary cardiac cells, we found that arboviruses from diverse viral families were able to replicate within these cells. Interestingly, we noted that while the closely related alphavirus Mayaro virus (MAYV) could replicate to high titers in primary human cardiac microvascular endothelial cells, pulmonary, and brain endothelial cells, the Indian Ocean Lineage of CHIKV (CHIKV-IOL) was completely restricted in all endothelial cells tested. Upon further investigation, we discovered that this restriction occurs at both entry and egress stages. Additionally, we observed that compared to CHIKV, MAYV may antagonize or evade the innate immune response more efficiently in human cardiac endothelial cells to increase infection. Overall, this study explores the tropism of arboviruses in human primary cardiac cells and characterizes the strain-specific restriction of CHIKV-IOL in human endothelial cells. Further work is needed to understand how the differential restriction of alphaviruses in human endothelial cells impacts pathogenesis in a living model, as well as the specific host factors responsible.Author SummaryMosquito-borne viruses, such as those within the alphavirus genus, are an ongoing concern to human health globally. While we have recently begun to explore the mechanisms of CHIKV-induced pathology in the heart, little is known about other arboviruses and alphaviruses related to CHIKV. Here, we identified that multiple cardiac cell types are susceptible to infection by several arboviruses important to public health. Specifically, we noted differences in how two related viruses, CHIKV and MAYV, infect endothelial cells from multiple origins. This work highlights the potential for other emerging arboviruses, in addition to CHIKV, to directly infect cardiac tissue. Moreover, it emphasizes the ability for MAYV, an emerging virus that is less studied, to infect various endothelial cell types to high titers, suggesting the need for further research on MAYV pathogenesis. Finally, this research identifies differences in infection between individual strains of CHIKV, suggesting a finely tuned mechanism of restriction in endothelial cells that can be further explored, as well as the need to study use different viral strains for future alphavirus research.

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Obesity-Associated Changes in Immune Cell Dynamics During Alphavirus Infection Revealed by Single Cell Transcriptomic Analysis

Hameed,

Daamen,

Hossain

et al. 2024

Preprint

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Obesity induces diverse changes in host immunity, resulting in worse disease outcomes following infection with various pathogens, including arthritogenic alphaviruses. However, the impact of obesity on the functional landscape of immune cells during arthritogenic alphavirus infection remains unexplored. Here, we used single-cell RNA sequencing (scRNA-seq) to dissect the blood and tissue immune responses to Mayaro virus (MAYV) infection in lean and obese mice. Footpad injection of MAYV caused significant shifts in immune cell populations and induced robust expression of interferon response and proinflammatory cytokine genes and related pathways in both blood and tissue. In MAYV-infected lean mice, analysis of the local tissue response revealed a unique macrophage subset with high expression of IFN response genes that was not found in obese mice. This was associated with less severe inflammation in lean mice. These results provide evidence for a unique macrophage population that may contribute to the superior capacity of lean mice to control arthritogenic alphavirus infection.

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Pathophysiology of chikungunya virus infection associated with fatal outcomes

Cited by 8 publications

References 100 publications

Factors Related to the Chikungunya Outbreak (Extraordinary Events) in Wonoboyo Sub-District, Wonogiri District, Wonogiri District

Factors Related to the Chikungunya Outbreak (Extraordinary Events) in Wonoboyo Sub-District, Wonogiri District, Wonogiri District

Differential restriction of chikungunya virus in primary human cardiac endothelial cells occurs at multiple steps in the viral life cycle

Obesity-Associated Changes in Immune Cell Dynamics During Alphavirus Infection Revealed by Single Cell Transcriptomic Analysis

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