Pathophysiology of diabetic nephropathy

Vinod, PB

doi:10.1016/s2211-9477(12)70005-5

Cited by 48 publications

(37 citation statements)

References 90 publications

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“…Blood levels of HbA1c is a marker for disorders of glucose metabolism and/orls adequate control of blood glucose levels in diabetic patients [10,11]. In this study we found a significant increase of HbA1 in patients with diabetic nephropathy as compared to newly diagnosed T1D.…”

Section: Citationsupporting

confidence: 54%

Beyond Proteinuria: Apoptosis and Vitamin D3 State in Children with Diabetic Nephropathy

Burlaka¹

2016

J Clin Exp Pathol

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Diabetic nephropathy (DN) is the leading cause of death in patients with diabetes mellitus type I (T1D). Formation of diabetic nephropathy depends on the quality of blood glucose control.The aim of the study was to investigate the level of metabolic-hypoxic disorders, condition of apoptosis controlling system and Vitamin D3 levels in children with diabetic nephropathy.The study involved 36 children with type I diabetes and diabetic nephropathy (aged 6 to 17 years). The affinity of haemoglobin to oxygen determined by spectrophotometric method. The levels of the marker of cellular hypoxia HIF-1alfa, levels of the apoptotic factor caspase-3 studied by Western Blotting, Vitamin D3 levels were analyzed using ELISA assay.In the group of children with newly diagnosed T1D an increased rate of dissociation of oxygen and haemoglobin compared to the control group was detected. Children with DN the levels of this index were significantly lower than the control group. We show stage-dependent manner in increase of the cellular hypoxia and apoptotic effector caspase-3 levels. Both markers detected at significantly higher rate in DN patients as compared to T1D. Vitamin-D3 deficiency is documented in patients with DN.Thus, the development of DN in children is associated with violation of Hb/oxygen dissociation, a sign of the high degree of the Hb glycosylation resulting to the formation of cellular hypoxia and activation of apoptosis, as one of the basic mechanisms of cell damage kidneys in DN. All mentioned above disorders accompanied by Vitamin D3 deficiency.

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Section: Citationsupporting

confidence: 54%

Beyond Proteinuria: Apoptosis and Vitamin D3 State in Children with Diabetic Nephropathy

Burlaka¹

2016

J Clin Exp Pathol

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“…1 Diabetes is the major cause of CRF which in turn may lead to end-stage renal disease resulting in dialysis. 2 Diabetic nephropathy (DN) is one of the most common microvascular complications of type 1 and type 2 diabetes mellitus, and is the leading cause of end-stage renal disease worldwide. 3 Many factors contribute to the development of DN, including hyperglycemia, hypertension, obesity, a sedentary lifestyle, hereditary, smoking, and advancing age.…”

Section: Introductionmentioning

confidence: 99%

“…5 According to recent studies, activation of electron transport chain induced by hyperglycemia can result in an increase in formation of the reactive oxygen species (ROS), which is thought to be the initiating event in the development of complications in diabetes. 2 Hemodynamic changes, hypertrophy, extracellular matrix accumulation, growth factor/cytokine induction, ROS formation, podocyte damage, proteinuria and interstitial inflammation are the steps in the advancement of DN. 2 ROS are believed to play a key role because of their reactive chemical property to directly oxidize and damage DNA, protein, lipid, and carbohydrate in the pathogenesis of late diabetic complications.…”

Section: Introductionmentioning

confidence: 99%

“…2 Hemodynamic changes, hypertrophy, extracellular matrix accumulation, growth factor/cytokine induction, ROS formation, podocyte damage, proteinuria and interstitial inflammation are the steps in the advancement of DN. 2 ROS are believed to play a key role because of their reactive chemical property to directly oxidize and damage DNA, protein, lipid, and carbohydrate in the pathogenesis of late diabetic complications. 6 Also, 8-OHdG has been used as a marker in determining DNA damage caused by oxidative stress in recent years.…”

Section: Introductionmentioning

confidence: 99%

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Determination of oxidative stress and cellular inflammation in patients with diabetic nephropathy and non-diabetic nephropathy being administered hemodialysis treatment due to chronic renal failure

et al. 2014

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Objectives: We aimed to evaluate oxidative stress , malondialdehyde (MDA)] endothelial damage [asymmetric dimethylarginine (ADMA)] and markers of cellular inflammation [interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-a), neopterin (NP) and high-sensitivity C-reactive protein (hsCRP)] in patients with diabetic nephropathy (DN) and non-diabetic nephropathy who were being administered hemodialysis treatment because of chronic renal failure. Methods: In determining 8-OHdG, IL-6 and TNF-a levels, Enzyme-Linked Immuno-Sorbent Assay method was used. Serum MDA, ADMA and NP levels were determined by using high performance liquid chromatography (HPLC). And hs-CRP values were measured with nephelometric method. Results: Serum 8-OHdG and MDA levels were found statistically to have increased when compared with those of the control group in patients groups after dialysis. However, serum ADMA and neopterin levels were observed statistically to have decreased when compared with those of the control group in patients groups after dialysis. But, decreases on ADMA and neopterin levels are still much higher than those of control. IL-6 and TNF-a levels were found to have increased when compared with those of control group in patients groups before dialysis. Conclusion: The oxidative stress in patients with DN, who were being treated with hemodialysis due to chronic renal failure, was higher than that of non-DN patients who were being treated with hemodialysis. In contrast with this, inflammation occurring in non-DN patients was found to have been higher than that of in patients with DN.

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“…The exonic (g.869T > C) and promoter (g.-509C > T) polymorphisms have been associated with DN traits (Coll et al, 2004;Khalil et al, 2005;Prasad et al, 2007). As the public health impact of DN is expected to grow in the years to come due to the increasing prevalence of diabetes (Vinod, 2012) and moreover keeping in view the role of genetic predisposition for the development of these diseases, the present study aimed to determine the association of TGF-b1 g.869T > C and g.-509C > T polymorphisms in T2D and ESRD cases derived from T2D in the two geographically and ethnically different populations of North India.…”

Section: Introductionmentioning

confidence: 99%

Association of Transforming Growth Factor Beta-1 (TGF-β1) Genetic Variation with Type 2 Diabetes and End Stage Renal Disease in Two Large Population Samples from North India

Raina

Sikka

Kaur

et al. 2015

OMICS: A Journal of Integrative Biology

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Geographic and ethnic differences impart an immense influence on the genetic susceptibility to Type 2 diabetes (T2D) and diabetic nephropathy (DN). Transforming growth factor-beta1 (TGF-b1), a ubiquitously expressed pro-fibrotic cytokine plays a pivotal role in mediating the hypertrophic and fibrotic manifestations of DN. The present study is aimed to study the association of TGF-b1 g.869T > C (rs1800470) and g.-509C > T (rs1800469) polymorphism in T2D and end stage renal disease (ESRD) cases from the two geographically and ethnically different populations from North India. A total of 1313 samples comprising 776 samples from Punjab (204 with ESRD, 257 without ESRD, and 315 healthy controls) and 537 samples from Jammu and Kashmir (150 with ESRD, 187 without ESRD, and 200 controls) were genotyped for TGF-b1 (rs1800470 and rs1800469) using ARMS-PCR. The CC genotype of rs1800470 increased ESRD risk by 3.1-4.5-fold in both populations. However, for rs1800469, the TT genotype provided 5.5-fold risk towards ESRD cases from Jammu and Kashmir and no risk for the cases from Punjab. The haplotype C-T conferred nearly a 2-3-fold risk towards T2D and ESRD and diplotype CC-CT conferred a 4-fold risk towards ESRD. Our results conclude that TGF-b1 (rs1800470) may increase the risk of both ESRD and T2D in both populations, but TGF-b1 (rs1800469) provided risk for only ESRD in the population of Jammu and Kashmir. The present study is one of the large sample sized genetic association studies of T2D and ESRD from Indian population and adds to the scholarship on global health omics.

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Pathophysiology of diabetic nephropathy

Cited by 48 publications

References 90 publications

Beyond Proteinuria: Apoptosis and Vitamin D3 State in Children with Diabetic Nephropathy

Beyond Proteinuria: Apoptosis and Vitamin D3 State in Children with Diabetic Nephropathy

Determination of oxidative stress and cellular inflammation in patients with diabetic nephropathy and non-diabetic nephropathy being administered hemodialysis treatment due to chronic renal failure

Association of Transforming Growth Factor Beta-1 (TGF-β1) Genetic Variation with Type 2 Diabetes and End Stage Renal Disease in Two Large Population Samples from North India

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