Pathophysiology of Escherichia coli ventilator-associated pneumonia: implication of highly virulent extraintestinal pathogenic strains

Messika, Jonathan; Magdoud, Fatma; Clermont, Olivier; Margetis, Dimitri; Gaudry, Stéphane; Roux, Damien; Branger, Catherine; Dreyfuss, Didier; Denamur, Erick; Ricard, Jean‐Damien

doi:10.1007/s00134-012-2699-5

Cited by 29 publications

(31 citation statements)

References 50 publications

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“…In this work, we focused on E. coli for the following reasons: i) oropharyngeal colonization with (9,10). To complete this proof of concept study, we reasoned it was necessary to also assess the efficacy of bacteriophage on strain PDP302, a clinical strain responsible for a VAP in an ICU patient.…”

Section: Discussionmentioning

confidence: 99%

“…Strain PDP302 was isolated from an ICU patient under mechanical ventilation who developed a ventilator-associated pneumonia and belongs to a well characterized collection (9).…”

Section: Escherichia Coli Strainsmentioning

confidence: 99%

“…Next, we searched for an E. coli VAP strain displaying equivalent characteristic to the archetypal strain 536 and identified strain PDP302 from our collection (9). In vitro, this clinical strain was as efficiently infected by bacteriophage 536_P7 as strain 536 (EOP of 1, see methods and SDC).…”

Section: In Vitro Adaptation Of Bacteriophages 536_p7 Is Required To mentioning

confidence: 99%

“…In the ICU setting, E. coli strains involved in VAP belong predominantly to the B2 phylogenetic group and possess a high number of virulence factors (9). Interestingly, a number of these strains belonged to subgroup III (Sequence Type 127) including the highly virulent archetypal strain 536 (10).…”

Section: Introductionmentioning

confidence: 99%

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Treatment of Highly Virulent Extraintestinal Pathogenic Escherichia coli Pneumonia With Bacteriophages*

Dufour

Debarbieux

Fromentin³

et al. 2015

Critical Care Medicine

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Section: Discussionmentioning

confidence: 99%

“…Strain PDP302 was isolated from an ICU patient under mechanical ventilation who developed a ventilator-associated pneumonia and belongs to a well characterized collection (9).…”

Section: Escherichia Coli Strainsmentioning

confidence: 99%

Section: In Vitro Adaptation Of Bacteriophages 536_p7 Is Required To mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Treatment of Highly Virulent Extraintestinal Pathogenic Escherichia coli Pneumonia With Bacteriophages*

Dufour

Debarbieux

Fromentin³

et al. 2015

Critical Care Medicine

Self Cite

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“…Membership of these phylogenetic entities is of particular interest, as there is a relationship between the genetic background of a strain and its virulence factors (VFs) (Escobar-Páramo et al, 2004a). For example, some sequence types (STs) within the B2 phylogroup, such as ST137, include strains with numerous extra-intestinal genes and high virulence potential (Messika et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic, virulence and antibiotic resistance characteristics of commensal strain populations of Escherichia coli from community subjects in the Paris area in 2010 and evolution over 30 years

et al. 2016

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Editor's Choice Phylogenetic, virulence and antibiotic resistance characteristics of commensal strain populations of Escherichia coli from community subjects in the Paris area in 2010 and evolution over 30 years It is important to study commensal populations of Escherichia coli because they appear to be the reservoir of both extra-intestinal pathogenic E. coli and antibiotic resistant strains of E. coli. We studied 279 dominant faecal strains of E. coli from 243 adults living in the community in the Paris area in 2010. The phylogenetic group and subgroup [sequence type complex (STc)] of the isolates and the presence of 20 virulence genes were determined by PCR assays. The O-types and resistance to 18 antibiotics were assessed phenotypically. The B2 group was the most frequently recovered (34.0 %), followed by the A group (28.7 %), and other groups were more rare. The most prevalent B2 subgroups were II (STc73), IV (STc141), IX (STc95) and I (STc131), with 22.1, 21.1, 16.8 and 13.7 %, respectively, of the B2 group strains. Virulence factors (VFs) were more common in B2 group than other strains. One or more resistances were found in 125 strains (44.8 % of the collection) but only six (2.2 % of the collection) were multiresistant; no extended-spectrum beta-lactamase-producing strain was isolated. The C phylogroup and clonal group A strains were the most resistant. No trade-off between virulence and resistance was evidenced. We compared these strains with collections of strains gathered under the same conditions 30 and 10 years ago. There has been a parallel and linked increase in the frequency of B2 group strains (from 9.4 % in 1980, to 22.7 % in 2000 and 34.0 % in 2010) and of VFs. Antibiotic resistance also increased, from 22.6 % of strains resistant to at least one antibiotic in 1980, to 31.8 % in 2000 and 44.8 % in 2010; resistance to streptomycin, however, remained stable. Commensal human E. coli populations have clearly evolved substantially over time, presumably reflecting changes in human practices, and particularly increasing antibiotic use.

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Year in review in Intensive Care Medicine 2012. II: Pneumonia and infection, sepsis, coagulation, hemodynamics, cardiovascular and microcirculation, critical care organization, imaging, ethics and legal issues

et al. 2013

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Pathophysiology of Escherichia coli ventilator-associated pneumonia: implication of highly virulent extraintestinal pathogenic strains

Cited by 29 publications

References 50 publications

Treatment of Highly Virulent Extraintestinal Pathogenic Escherichia coli Pneumonia With Bacteriophages*

Treatment of Highly Virulent Extraintestinal Pathogenic Escherichia coli Pneumonia With Bacteriophages*

Phylogenetic, virulence and antibiotic resistance characteristics of commensal strain populations of Escherichia coli from community subjects in the Paris area in 2010 and evolution over 30 years

Year in review in Intensive Care Medicine 2012. II: Pneumonia and infection, sepsis, coagulation, hemodynamics, cardiovascular and microcirculation, critical care organization, imaging, ethics and legal issues

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