Pathotyping of a Newcastle disease virus isolated from peacock (Pavo cristatus)

Abstract

This report describes Newcastle disease in peacock and the isolation and characterization of the virus. The virus had an intracerbral pathogenicity index of 1.71 and mean death time of 47 h. The isolate had multiple basic amino acids at the fusion protein cleavage site sequence ((110)GGRRQRRFIG(119)) with a phenylalanine at residue 117. Biological and molecular characterization revealed that the virus is velogenic. Phylogenetic analysis placed the isolate in genotype II.

Detection and molecular characterization of Newcastle disease virus in peafowl (Pavo cristatus) in Haryana State, India

“…Mortality in peacock was observed with congestion of lungs and intestinal mucosa. NDV outbreaks in peafowls have been reported earlier from India and other part of the world [14,28]. Contrary to our findings Vijayarani et al [28] reported the absence of characteristics NDV signs in birds.…”

“…NDV outbreaks in peafowls have been reported earlier from India and other part of the world [14,28]. Contrary to our findings Vijayarani et al [28] reported the absence of characteristics NDV signs in birds. Postmortem lesions that included congestion in lungs and intestinal mucosa were similar to those observed previously by Vijayarani et al [28] and were also indicative of NDV infection.…”

Analysis of the Fusion Protein Cleavage Site of Newcastle disease virus Isolates from India Reveals Preliminary Evidence for the Existence of II, VI and VII Genotypes

“…There is only one report with respect to the genotyping of a NDV isolate from peacock in India employing the well characterized molecular determinant the FPCS [42]. This report points to the prevalence and circulation of genotype II virus in India.…”

“…Most of the reports had not only looked into the biological characteristics of the virus but also sequenced the FPCS to determine the virulence of the virus [21,22,24,42]. There are also reports on isolation, biological characterization and FPCS sequence based pathotyping of NDV from backyard chicken [6] and other species of birds such as pigeons [18,24,40,42,46] Japanese quail [21], Turkey [37], Duck [47], crow [31], Emu [23] and Ostrich [45].…”

“…Most of the reports had not only looked into the biological characteristics of the virus but also sequenced the FPCS to determine the virulence of the virus [21,22,24,42]. There are also reports on isolation, biological characterization and FPCS sequence based pathotyping of NDV from backyard chicken [6] and other species of birds such as pigeons [18,24,40,42,46] Japanese quail [21], Turkey [37], Duck [47], crow [31], Emu [23] and Ostrich [45]. In fact there is also an isolation of a lentogenic strain of NDV from backyard chicken that has been completely characterised with respect to its biological and sequence (both the fusion (F) and Haemagglutinin neuraminidase (HN) genes) characteristics [6,32,34,43] and the same has been used in preparing an oral pellet vaccine.…”