Pathway of Dephosphorylation of <i>myo</i>-Inositol Hexakisphosphate by Phytases of Legume Seeds

Greiner, Ralf; Alminger, Marie; Carlsson, Nils‐Gunnar; Múzquiz, M.; Burbano, Carmen; Cuadrado, Carmen; Pedrosa, Mercedes M.; Goyoaga, Carmen

doi:10.1021/jf025620t

Cited by 34 publications

(18 citation statements)

References 35 publications

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“…Phosphate Inhibition-PhyAsr (1 M) was incubated with 2-50 mM InsP 6 in the presence and absence of 1.0 and 10.0 mM inorganic phosphate for 5 min, and the products were separated using a CarboPax-100 column (Dionex) following standard protocols (27). 6 Complex-To examine the interactions between PhyAsr and its substrate, InsP 6 , we have determined the high resolution structure (1.6 Å) of the inactive C252S mutant (active-site nucleophile) in complex with InsP 6 (PDB code 3MMJ).…”

Section: Methodsmentioning

confidence: 99%

Substrate Binding in Protein-tyrosine Phosphatase-like Inositol Polyphosphatases

Gruninger

Dobing

Smith

et al. 2012

Journal of Biological Chemistry

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Background: PTP-like inositol polyphosphatases hydrolyze myo-inositol hexakisphosphate via an ordered pathway. Results: X-ray structures in complex with substrates and fluorescence binding reveal novel features of the kinetic mechanism. Conclusion: PTP-like inositol polyphosphatases have a two-step binding mechanism that facilitates specificity and catalysis. Significance: Structural and binding studies are essential for understanding complex kinetic mechanisms.

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Section: Methodsmentioning

confidence: 99%

Substrate Binding in Protein-tyrosine Phosphatase-like Inositol Polyphosphatases

Gruninger

Dobing

Smith

et al. 2012

Journal of Biological Chemistry

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“…The Pantoea agglomerans and Saccharomyces cerevisiae phytases were purified as described previously (9,12).…”

Section: Chemicalsmentioning

confidence: 99%

Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9

Greiner¹,

Silva

Couri

2009

Braz. J. Microbiol.

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An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maximal phytate-degrading activity at pH 5.0. Optimum temperature for the degradation of phytate was 55°C. The kinetic parameters for the hydrolysis of sodium phytate were determined to be K M = 54 µmol l -1 and k cat = 190 sec -1 at pH 5.0 and 37°C.The purified enzyme was rather specific for phytate dephosphorylation. It was shown that the phytase preferably dephosphorylates myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-Ins(1,2,4,5,6)P 5 , D-Ins(1,2,5,6)P 4 , D-Ins(1,2,6)P 3 , D-Ins(1,2)P 2 to finally Ins(2)P.

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“…Some inositol phosphates, including IP 6 from soybean, have been suggested to have beneficial health effects, such as amelioration of heart diseases by controlling hypercholesterolemia and atherosclerosis, prevention of kidney stone formation and a reduced risk of colon cancer (Greiner et al, 2002).…”

Section: Inositol Phosphatesmentioning

confidence: 99%

Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)

Múzquiz

Guillamón

Burbano

et al. 1970

Span. j. agric. res.

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A new lupin from Valencia (Spain) has been described recently. The new species Lupinus mariae-josephi H. Pascual (Fabaceae) differs from any other Mediterranean and North American taxon in banner position, flower colour and inflorescence structure. This species was found in a calcareous area, and when it was grown in experimental fields it has always performed better in alkaline soils. This work attempts to establish the content of protein, oil and nutritionally active factors (alkaloids, α-galactosides and inositol phosphates) in this new species of lupin, to investigate differences with other Lupinus ssp. The analysed raw seed samples of this new species showed a total protein content of 329.4 g kg -1 and oil content of 54.0 g kg -1 . The total content of alkaloids, α-galactosides and phytate was similar to other bitter Lupinus species being lupanine, stachyose and myo-inositol hexaphosphate (IP 6 ) the main bioactive compounds. However, the profile of alkaloids and proteins, which is an important chemotaxonomic criterion, was characteristic of this species. It is worth to notice that this Lupinus is able to grow in calcareous soils what makes this species very promising for soil recovery.Additional keywords: alkaloids; fatty acids; lupin; phytate; protein pattern; α-galactosides. ResumenComposición química de una nueva especie de Lupinus encontrada en España: Lupinus mariae-josephi H. Pascual (Fabaceae) Recientemente se ha descrito una nueva especie de Lupinus en Valencia (España). Esta nueva especie, Lupinus mariae-josephi H. Pascual (Fabaceae) difiere de otras especies de Lupinus del área mediterránea o de Norteamérica en la posición del estandarte, el color de la flor y la estructura de la inflorescencia. Fue encontrada en un área calcárea, y en estos últimos años, cuando se ha cultivado, ha crecido en suelos calizos. El objetivo de este trabajo ha sido estudiar el contenido de proteína, grasa y factores nutricionalmente activos (alcaloides, α-galactósidos y fitatos) en esta nueva especie, para ver las diferencias que existen con otras especies de Lupinus. Los resultados han mostrado un contenido en proteína de 329,4 g kg -1 y un contenido en grasa de 54,0 g kg -1 . El contenido total de alcaloides, α-galactósidos y fitatos fue similar a otras especies amargas de Lupinus siendo la lupanina, la estaquiosa y el mioinositol hexafosfato (IP 6 ) los principales componentes bioactivos. Sin embargo, el perfil de alcaloides y el perfil proteico, que son un importante criterio taxonómico, fueron característicos de esta especie. Es importante resaltar que esta especie de Lupinus es capaz de crecer en suelos calizos, lo que la hace muy prometedora para la recuperación de suelos.

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Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases of Legume Seeds

Cited by 34 publications

References 35 publications

Substrate Binding in Protein-tyrosine Phosphatase-like Inositol Polyphosphatases

Substrate Binding in Protein-tyrosine Phosphatase-like Inositol Polyphosphatases

Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9

Chemical composition of a new Lupinus species found in Spain, Lupinus mariae-josephi H. Pascual (Fabaceae)

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