Pathway of Glucose Fermentation in Relation to the Taxonomy of Bifidobacteria

Vries, Wytske de; Stouthamer, A. H.

doi:10.1128/jb.93.2.574-576.1967

Cited by 118 publications

(51 citation statements)

References 3 publications

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“…Thus, in cases where all of the pyruvate was cleaved rather than reduced, no lactate was formed. Because this pathway does not occur in any other species of the genus Lactobacillus, de Vries and Stouthamer (179), in agreement with some previous workers, suggested that classification of the bifidobacteria in this genus is not justified. The same alternative route of glucose dissimulation has been established in L. bifidus var.…”

Section: Biochemical Characteristicssupporting

confidence: 79%

Biology of the bifidobacteria.

Poupard¹,

Husain²,

Norris³

1973

Bacteriological Reviews

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Section: Biochemical Characteristicssupporting

confidence: 79%

Biology of the bifidobacteria.

Poupard¹,

Husain²,

Norris³

1973

Bacteriological Reviews

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“…Thus, in addition to members of the Enterobacteriaceae and a number of other genera that are facultative or obligate anaerobes (for a review, see reference 21), this system is present in homofermentative lactic acid bacteria. It is absent, however, in heterofermentative lactic acid bacteria which ferment glucose via the pentose phosphoketolase pathway (4,31), or via the hexose phosphoketolase pathway (5,31). It is also absent in Z. mobilis, an anerobic pseudomonad that ferments glucose via the Entner-Doudoroff pathway (7,31).…”

Section: Uptake Of 2-deoxy-d-glucose Resting Cells Of L Casei Accummentioning

confidence: 99%

“…However, Komberg and Smith (13) were the first to emphasize the importance of the PEP yield from glycolysis, when they suggested that an Escherichia coli mutant that was defective in the EMP pathway, and was thus forced to metabolize glucose via the hexose monophosphate shunt, grew sparsely on glucose because of a deficiency in PEP required both for transport and for biosynthesis. If this line of reasoning is pursued further, one would predict that the PEP:glucose phosphotransferase system would be operative in those organisms fermenting glucose via the EMP pathway, where there is a yield of two molecules of PEP per molecule of glucose fermented, but would be absent in those organisms fermenting glucose by other pathways, such as the phosphoketolase pathway (4,5,31) or the anaerobic Entner-Doudoroff pathway (7,31), where the PEP yield is only one molecule of PEP per molecule of glucose fermented. This report shows that this prediction is realized.…”

mentioning

confidence: 99%

Distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria

Romano¹,

Trifone²,

Brustolon³

1979

J Bacteriol

122

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A number of selected fermentative bacteria were surveyed for the presence of the phosphoenolpyruvate:glucose phosphotransferase system, with particular attention to those organisms which ferment glucose by pathways other than the Embden-Meyerhof-Parnas pathway. The phosphoenolpyruvate:glucose phosphotransferase system was found in all homofermentative lactic acid bacteria tested that ferment glucose via the Embden-Meyerhof-Parnas pathway, but in none of a group of heterofermentative species of Lactobacillius or Leuconostoc, which ferment glucose via the phosphoketolase pathway. A phosphoenolpyruvate:glucose phosphotransferase system was also absent in Zymomonas mobilis, which ferments glucose via an anaerobic Entner-Doudoroff pathway. It thus appears that the phosphotransferase mode of glucose transport is limited to bacteria with the Embden-Meyerhof-Parnas mode of glucose fermentation.

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“…The phosphoketolase pathway ( Fig. 5A ), termed the “bifid shunt” (21) in a Bifidobacterium (22), provides an alternative to glycolysis and the pentose phosphate pathway for sugar utilization. In this pathway, the phosphoketolase enzyme splits xylulose-5-phosphate into glyceraldehyde-3-phosphate and acetyl-phosphate (23).…”

Section: Resultsmentioning

confidence: 99%

Multi-omic analysis of medium-chain fatty acid synthesis byCandidatusWeimerbacter bifidus, gen. nov., sp. nov., andCandidatusPseudoramibacter fermentans, sp. nov.

Scarborough

Myers

Donohue

et al. 2019

Preprint

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Chain elongation is emerging as a bioprocess to produce valuable medium-chain fatty acids (MCFA; 6 to 8 carbons in length) from organic waste streams by harnessing the metabolism of anaerobic microbiomes. Although our understanding of chain elongation physiology is still evolving, the reverse β-oxidation pathway has been identified as a key metabolic function to elongate the intermediate products of fermentation to MCFA. Here, we describe two chain-elongating microorganisms that were enriched in an anaerobic microbiome transforming the residues from a lignocellulosic biorefining process to short- and medium-chain fatty acids. Based on a multi-omic analysis of this microbiome, we predict that Candidatus Weimerbacter bifidus, gen. nov., sp. nov. used xylose to produce MCFA, whereas Candidatus Pseudoramibacter fermentans, sp. nov., used glycerol and lactate as substrates for chain elongation. Both organisms are predicted to use an energy conserving hydrogenase to improve the overall bioenergetics of MCFA production.IMPORTANCEMicrobiomes are vital to human health, agriculture, environmental processes, and are receiving attention as biological catalysts for production of renewable industrial chemicals. Chain elongation by MCFA-producing microbiomes offer an opportunity to produce valuable chemicals from organic streams that otherwise would be considered waste. However, the physiology and energetics of chain elongation is only beginning to be studied, and we are analyzing MCFA production by self-assembled communities to complement the knowledge that has been acquired from pure culture studies. Through a multi-omic analysis of an MCFA-producing microbiome, we characterized metabolic functions of two chain elongating bacteria and predict previously unreported features of this process.

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Pathway of Glucose Fermentation in Relation to the Taxonomy of Bifidobacteria

Cited by 118 publications

References 3 publications

Biology of the bifidobacteria.

Biology of the bifidobacteria.

Distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria

Multi-omic analysis of medium-chain fatty acid synthesis byCandidatusWeimerbacter bifidus, gen. nov., sp. nov., andCandidatusPseudoramibacter fermentans, sp. nov.

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