Background To explore effectiveness of clinical pathway teaching combine OSCE in the training of new ICU nurses.Methods To selected 52 new nurses who joined ICU of Peking University Shenzhen hospital from August 2020 to January 2022, and divided the subjectsc ronological into two groups. The experimental group used the clinical pathway teaching and combined with the OSCE evaluation, while the control group took the routine training method and assessment method. Used the 6-D scale and the CNCQ to collect new ICU nurses’data before and after a two-month cycle training.Results In this study, the average score of the theoretical exam in experimental group was 85.72 (76.00,94.00), which is higher than control group( 78.98 (73.00,84.00)), The difference was significant (P < 0.05). New nurses of experimental group had higher scores on the CNCQ’s items after training(93.46 ± 11.20)than before(75.46 ± 11.19), differences were statistically significant(P < 0.001). There was astatistical difference(P = 0.024) in the scores of control group before(85.92 ± 12.19) and after(93.46 ± 11.20) training. New nurses of experimental grouphad higher scores on the 6-D scale’s items after training(185.00 ± 39.98)than before(154.19 ± 38.32), differences were statistically significant(P < 0.05). However, there was no statistical difference in the scores of control group before and after training.Conclusion Clinical pathway teaching combined with OSCE can improve the theoretical knowledge level and clinical comprehensive ability of new ICU nurses, and promote good nursing behaviour for them. Recognition of ability toplayin the nursing pos can affect their nursing behavior. Clinical pathway teaching combined with OSCE can fill the gap in nursing behaviours before and after training, such as nurse teaching collaboration, planning/evaluation, interpersonal communication, professional development. But the improvement of leadership and critical care ability still need enough clinical practice.