Pathways in bacterial and archaeal communities dictated by ammonium stress in a high solid anaerobic digester with dewatered sludge

Li, Ning; He, Jin; Yan, Han; Chen, Sisi; Dai, Xiaohu

doi:10.1016/j.biortech.2017.05.094

Cited by 57 publications

(25 citation statements)

References 32 publications

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“…Recent studies have applied metagenomics to study the microbial community response to inhibitors such as ammonia (Gao et al, 2015;Li et al, 2017a;Werner et al, 2014), LCFA (Beale et al, 2016;Kougias et al, 2016), temperature (Beale et al, 2016;Pap et al, 2015), and VFA (Mosbaek et al, 2016). A study that used [U-13 C] labelled acetate and metagenomics to evaluate VFA inhibition observed that acetate was consumed by Methanosarcina, Methanoculleus, and five subspecies of Clostridia that contained the ftfhs gene (Mosbaek et al, 2016).…”

Section: Metagenomicsmentioning

confidence: 99%

“…However, a gradual increase to thermophilic temperature enriched hydrogenotrophic methanogens alongside an increased abundance of hydrogen producing Fe-hydrogenases associated with syntrophic and fermentative bacteria. Metagenomics has similarly been used to evaluate methanogenic pathways in response to elevated ammonia, with a shift from acetoclastic to hydrogenotrophic methanogenesis and SAO reported (Gao et al, 2015;Li et al, 2017a;Werner et al, 2014). Beyond revealing the shifts in microbial community composition and metabolic potential, metagenomics has also been effective at elucidating communities that would have remained uncharacterized without using non-targeted approaches (Guermazi et al, 2008;Kougias et al, 2017).…”

Section: Metagenomicsmentioning

confidence: 99%

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Inhibition of anaerobic digestion processes: Applications of molecular tools

Amha

Anwar

Brower

et al. 2018

Bioresource Technology

119

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Section: Metagenomicsmentioning

confidence: 99%

Section: Metagenomicsmentioning

confidence: 99%

Inhibition of anaerobic digestion processes: Applications of molecular tools

Amha

Anwar

Brower

et al. 2018

Bioresource Technology

119

View full text Add to dashboard Cite

“…Algal particle scavenging MGIIa also have the potential for motility or adhesion via an archaeal flagellum-based system (Li, He, Yan, Chen, & Dai, 2017;Rinke et al, 2018;Tully, 2019). For MGIIb, several genomic features support the idea of aerobic heterotrophic metabolisms based on different substrates like proteins, carbohydrates, fatty acids, and lipids (Li et al, 2017;Rinke et al, 2018;Tully, 2019).…”

mentioning

confidence: 99%

Genomic ecology of Marine Group II, the most common marine planktonic Archaea across the surface ocean

et al. 2019

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Planktonic Archaea have been detected in all the world's oceans and are found from surface waters to the deep sea. The two most common Archaea phyla are Thaumarchaeota and Euryarchaeota. Euryarchaeota are generally more common in surface waters, but very little is known about their ecology and their potential metabolisms. In this study, we explore the genomic ecology of the Marine Group II (MGII), the main marine planktonic Euryarchaeota, and test if it is composed of different ecologically relevant units. We re‐analyzed Tara Oceans metagenomes from the photic layer and the deep ocean by annotating sequences against a custom MGII database and by mapping gene co‐occurrences. Our data provide a global view of the distribution of Euryarchaeota, and more specifically of MGII subgroups, and reveal their association to a number of gene‐coding sequences. In particular, we show that MGII proteorhodopsins were detected in both the surface and the deep chlorophyll maximum layer and that different clusters of these light harvesting proteins were present. Our approach helped describing the set of genes found together with specific MGII subgroups. We could thus define genomic environments that could theoretically describe ecologically meaningful units and the ecological niche that they occupy.

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“…(a)]. Metagenomics studies of municipal and industrial anaerobic digesters have shown that Archaea usually represent 4–7% of total reads or around 3–9% considering only classified microorganisms . Nevertheless, the increase of the Archaea component in the combined inoculum up to 19.4% (Fig.…”

Section: Resultsmentioning

confidence: 99%

Methane potential and metagenomics of wastewater sludge and a methane‐producing landfill solid sample as microbial inocula for anaerobic digestion of food waste

Sillas‐Moreno

Senés‐Guerrero

Pacheco

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Anaerobic digestion of food waste is influenced by the selection of an adequate microbial inoculum. In this study, wastewater sludge, a methane (CH4)‐producing landfill solid sample and the combination of both [1:1 volatile solid (VS)] were tested as inocula to treat food waste modelled to the Mexican diet. Hence, biochemical methane potential assays at inoculum to substrate (I:S) ratios of 1–3 and each inoculum metagenome were determined. RESULTS Methane production was optimal at I:S = 1 for sludge and the combined inoculum, whereas for landfill solid sample increasing the proportion of inoculum was beneficial up to I:S = 2. The landfill solid sample produced more CH4 than commonly used sludge (311.5 ± 11.1 and 282.5 ± 18.1 mL CH4 g−1 VS, respectively) but presented an adaptation phase (four days). Combining inocula (I:S = 1) produced a synergistic effect in CH4 yield (374.5 ± 10.5 mL CH4 g−1 VS), biogas quality (58.0 ± 0.3% CH4) and COD removal (90.0 ± 0.6%) without start‐up time. Moreover, an enriched microbial community high in Archaea and Bacteria was observed, probably because of the incorporation of predominant members of each inoculum (Bacteroidetes, Firmicutes and Euryarchaeota in sludge, and Proteobacteria and Actinobacteria in landfill solid sample). Archaea was represented mainly by the genus Methanosaeta (order Methanosarcinales), suggesting that methanogenesis occurred by the acetoclastic pathway. CONCLUSION Food waste from Mexican diet was a suitable feedstock for biogas generation. Optimal conditions were observed when combining both inocula. Further studies on population dynamics during digestion would help understand the synergistic effect for effective industrial application. © 2018 Society of Chemical Industry

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Pathways in bacterial and archaeal communities dictated by ammonium stress in a high solid anaerobic digester with dewatered sludge

Cited by 57 publications

References 32 publications

Inhibition of anaerobic digestion processes: Applications of molecular tools

Inhibition of anaerobic digestion processes: Applications of molecular tools

Genomic ecology of Marine Group II, the most common marine planktonic Archaea across the surface ocean

Methane potential and metagenomics of wastewater sludge and a methane‐producing landfill solid sample as microbial inocula for anaerobic digestion of food waste

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