Concentrations of fifteen trace metals including Aluminum (Al), Vanadium (V), Chromium (Cr), Manganese (Mn), Iron (Fe), Strontium (Sr), Molybdenum (Mo), Silver (Ag), Cadmium (Cd), Tin (Sn), Caesium (Cs), Barium (Ba), Lead (Pb), Bismuth (Bi) and Uranium (U) were investigated in water samples collected from sixteen sampling locations in the Lubumbashi river basin and five locations in Kafubu, Kimilolo and Kinkalabwamba rivers during February, March and April 2016. Chemical analyses of the samples were carried out using ICP-MS (Inductively Coupled Plasma-Mass Spectrometer). Water pH was determined using a pH-meter and pH values ranged from 4.2 to 7.8. The highest mean trace metal levels of water were 5,515. Trace metal contamination of water of the studied rivers, channel and springs might be partially attributed to natural processes, unplanned urbanization, poor waste management and mostly to abandoned and ongoing mining and ore processing activities in Lubumbashi city.