Patient-derived breast model repository, a tool for hyperthermia treatment planning and applicator design

Androulakis, Ioannis; Sümser, Kemal; Machielse, Melanie N D; Koppert, L.; Jager, Agnes; Nout, R.A.; Franckena, Martine; Rhoon, Gerard C. van; Curto, Sergio

doi:10.1080/02656736.2022.2121862

Cited by 8 publications

(3 citation statements)

References 40 publications

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“…The clinical hyperthermia apparatus is usually composed by a microwave unit and an applicator [ 50 ]. Clarifying the molecular mechanism can help determine the most appropriate technique of hyperthermia treatment planning.…”

Section: Discussionmentioning

confidence: 99%

Hyperthermia promotes M1 polarization of macrophages via exosome-mediated HSPB8 transfer in triple negative breast cancer

Liu

Liang

et al. 2023

Discov Onc

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Purpose To investigate the mechanism underlying the modulation of M1 macrophage polarization by exosomes released from hyperthermia-treated triple-negative breast cancer (TNBC) cells. Materials and methods In this study, the effects of hyperthermia on TNBC cells were examined using cell counting kit-8, apoptosis, and cell cycle assays. Transmission electron microscopy was used to identify the structure of exosomes, while bicinchoninic acid and nanoparticle tracking analysis were used to detect particle size and amounts of exosomes released after hyperthermia. The polarization of macrophages incubated with exosomes derived by hyperthermia-pretreated TNBC cells were assessed by RT-qPCR and flow cytometry analysis. Next, RNA sequencing was performed to determine the targeting molecules changed in hyperthermia-treated TNBC cells in vitro. Finally, the mechanism underlying the modulation of macrophage polarization by exosomes derived from hyperthermia-treated TNBC cells was examined by using RT-qPCR, immunofluorescence and flow cytometry analysis. Results Hyperthermia markedly reduced cell viability in TNBC cells and promoted the secretion of TNBC cell-derived exosomes. The hub genes of hyperthermia-treated TNBC cells were significantly correlated with macrophage infiltration. Additionally, hyperthermia-treated TNBC cell-derived exosomes promoted M1 macrophage polarization. Furthermore, the expression levels of heat shock proteins, including HSPA1A, HSPA1B, HSPA6, and HSPB8, were significantly upregulated upon hyperthermia treatment, with HSPB8 exhibiting the highest upregulation. Moreover, hyperthermia can induce M1 macrophage polarization by promoting exosome-mediated HSPB8 transfer. Conclusion This study demonstrated a novel mechanism that hyperthermia can induce M1 polarization of macrophages via exosome-mediated HSPB8 transfer. These results will help with future development of an optimized hyperthermia treatment regime for clinical application, especially for combination treatment with immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Hyperthermia promotes M1 polarization of macrophages via exosome-mediated HSPB8 transfer in triple negative breast cancer

Liu

Liang

et al. 2023

Discov Onc

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“…Based on the authors' knowledge, there has not been any previous work studying the effect of antenna polarization on the focusing result of an antenna array used in microwave hyperthermia. The antennas in the arrays of traditional FMBH works are generally configured in the same polarization [28,29]. There are some related works investigating the antenna polarization on the power distribution uniformity in biological samples for microwave-induced thermoacoustic imaging [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Improvement of Phased Antenna Array Applied in Focused Microwave Breast Hyperthermia

Wang,

Xi,

et al. 2024

Sensors

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Focused microwave breast hyperthermia (FMBH) employs a phased antenna array to perform beamforming that can focus microwave energy at targeted breast tumors. Selective heating of the tumor endows the hyperthermia treatment with high accuracy and low side effects. The effect of FMBH is highly dependent on the applied phased antenna array. This work investigates the effect of polarizations of antenna elements on the microwave-focusing results by simulations. We explore two kinds of antenna arrays with the same number of elements using different digital realistic human breast phantoms. The first array has all the elements’ polarization in the vertical plane of the breast, while the second array has half of the elements’ polarization in the vertical plane and the other half in the transverse plane, i.e., cross polarization. In total, 96 sets of different simulations are performed, and the results show that the second array leads to a better focusing effect in dense breasts than the first array. This work is very meaningful for the potential improvement of the antenna array for FMBH, which is of great significance for the future clinical applications of FMBH. The antenna array with cross polarization can also be applied in microwave imaging and sensing for biomedical applications.

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“…The tumor in this scan was segmented and made available as a separate model. Recently, a repository containing models of twenty-two breast cancer patients was made available for the development of breast cancer hyperthermia devices and treatment planning [45]. These models include anatomically accurate representations of skin, bone, fat, fibroglandular, and muscle tissues of both breasts, as well as malignant tumors.…”

Section: Introductionmentioning

confidence: 99%

Repository of MRI-derived models of the breast with single and multiple benign and malignant tumors for microwave imaging research

Pelicano,

Gonçalves,

Castela

et al. 2024

PLoS ONE

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The diagnosis of breast cancer through MicroWave Imaging (MWI) technology has been extensively researched over the past few decades. However, continuous improvements to systems are needed to achieve clinical viability. To this end, the numerical models employed in simulation studies need to be diversified, anatomically accurate, and also representative of the cases in clinical settings. Hence, we have created the first open-access repository of 3D anatomically accurate numerical models of the breast, derived from 3.0T Magnetic Resonance Images (MRI) of benign breast disease and breast cancer patients. The models include normal breast tissues (fat, fibroglandular, skin, and muscle tissues), and benign and cancerous breast tumors. The repository contains easily reconfigurable models which can be tumor-free or contain single or multiple tumors, allowing complex and realistic test scenarios needed for feasibility and performance assessment of MWI devices prior to experimental and clinical testing. It also includes an executable file which enables researchers to generate models incorporating the dielectric properties of breast tissues at a chosen frequency ranging from 3 to 10 GHz, thereby ensuring compatibility with a wide spectrum of research requirements and stages of development for any breast MWI prototype system. Currently, our dataset comprises MRI scans of 55 patients, but new exams will be continuously added.

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Patient-derived breast model repository, a tool for hyperthermia treatment planning and applicator design

Cited by 8 publications

References 40 publications

Hyperthermia promotes M1 polarization of macrophages via exosome-mediated HSPB8 transfer in triple negative breast cancer

Hyperthermia promotes M1 polarization of macrophages via exosome-mediated HSPB8 transfer in triple negative breast cancer

Improvement of Phased Antenna Array Applied in Focused Microwave Breast Hyperthermia

Repository of MRI-derived models of the breast with single and multiple benign and malignant tumors for microwave imaging research

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